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学科主题: 临床医学
题名:
Interferon regulatory factor 8 functions as a tumor suppressor in renal cell carcinoma and its promoter methylation is associated with patient poor prognosis
作者: Zhang, Qian1,2; Zhang, Lian1,2; Li, Lili3,4; Wang, Zhaohui3; Ying, Jianming5,6; Fan, Yu1,2; Xu, Ben1,2; Wang, Lu1,2; Liu, Qianling1,2; Chen, Guangfu7; Tao, Qian3,4; Jin, Jie1,2
关键词: IRF8 ; Methylation ; Epigenetic ; Tumor suppressor ; Renal carcinoma
刊名: CANCER LETTERS
发表日期: 2014-11-28
DOI: 10.1016/j.canlet.2014.07.040
卷: 354, 期:2, 页:227-234
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: DNA METHYLATION ; TRANSCRIPTION FACTORS ; MYELOID CELLS ; PROTEIN ABUNDANCE ; CLEAR-CELL ; EXPRESSION ; GENE ; APOPTOSIS ; ACTIVATION ; MULTIPLE
英文摘要:

Interferon regulatory factor 8 (IRF8), as a central element of IFN-gamma-signaling, plays a critical role in tumor suppression. However, its expression and underlying molecular mechanism remain elusive in renal cell carcinoma (RCC). Here, we examined IRF8 expression and methylation in RCC cell lines and primary tumors, and further assessed its tumor suppressive functions. We found that IRF8 was widely expressed in human normal tissues including kidney, but frequently downregulated by promoter methylation in RCC cell lines. IRF8 methylation was detected in 25% of primary tumors, but not in adjacent non-malignant renal tissues, and associated with higher tumor nuclear grade of RCC. Ectopic expression of IRF8 inhibited colony formation and migration abilities of RCC cells, through inducing cell cycle G2/M arrest and apoptosis. IFN-gamma could induce IRF8 expression in RCC cells, together with increased cleaved-PARR We further found that IRF8 inhibited expression of oncogenes YAP1 and Survivin, as well as upregulated expression of tumor suppressor genes CASP1, p21 and PTEN. Collectively, our data demonstrate that IRF8 as a functional tumor suppressor is frequently methylated in RCC, and IRF8-mediated interferon signaling is involved in RCC pathogenesis. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 81272290 ; 81171971 ; 81372898 ; 81101492 ; JC201005270328A
项目资助者: National Natural Science Foundation ; Scientific Fund for Distinguished Young Scholars of Shenzhen
WOS记录号: WOS:000344127600003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55055
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
2.CUHK Shenzhen Res Inst, Shatin, Hong Kong, Peoples R China
3.Natl Res Ctr Genitourinary Oncol, Inst Urol, Beijing 100034, Peoples R China
4.Chinese Univ Hong Kong, Sir YK Pao Ctr Canc, Dept Clin Oncol, Canc Epigenet Lab,State Key Lab Oncol South China, Shatin, Hong Kong, Peoples R China
5.Chinese Acad Med Sci, Peking Union Med Coll, Inst Canc, Dept Pathol, Beijing 100021, Peoples R China
6.Chinese Acad Med Sci, Peking Union Med Coll, Canc Hosp, Beijing 100021, Peoples R China
7.PIA Gen Hosp, Med Sch Chinese PLA, Dept Urol, Beijing 100853, Peoples R China

Recommended Citation:
Zhang, Qian,Zhang, Lian,Li, Lili,et al. Interferon regulatory factor 8 functions as a tumor suppressor in renal cell carcinoma and its promoter methylation is associated with patient poor prognosis[J]. CANCER LETTERS,2014,354(2):227-234.
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