北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 北京大学干细胞研究中心  > 期刊论文
学科主题: 基础医学
题名:
The Egf Receptor-Sox2-Egf Receptor Feedback Loop Positively Regulates the Self-Renewal of Neural Precursor Cells
作者: Hu, Qikuan2,3; Zhang, Lirong2; Wen, Jinhua1,2; Wang, Shuling2; Li, Meiyu2; Feng, Ruopeng2; Yang, Xiaolong2; Li, Lingsong2
关键词: Neural procursor cells ; Egfr ; Sox2 ; Self-renewal
刊名: STEM CELLS
发表日期: 2010-02-01
DOI: 10.1002/stem.246
卷: 28, 期:2, 页:279-286
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
关键词[WOS]: EPIDERMAL-GROWTH-FACTOR ; SEX-DETERMINING REGION ; CENTRAL-NERVOUS-SYSTEM ; STEM-CELLS ; SUBVENTRICULAR ZONE ; PROGENITOR CELLS ; VASCULAR NICHE ; ASTROCYTES ; FOREBRAIN ; BRAIN
英文摘要:

The transcriptional factor Sox2 and epidermal growth factor receptor (Egfr)-mediated signaling are both required for self-renewal of neural precursor cells (NPCs). However, the mechanism by which these factors coordinately regulate this process is largely unknown. Here we show that Egfr-mediated signaling promotes Sox2 expression, which in turn binds to the Egfr promoter and directly upregulates Egfr expression. Knockdown of Sox2 by RNA interference downregulates Egfr expression and attenuates colony formation of NPCs, whereas overexpression of Sox2 elevates Egfr expression and promotes NPC self-renewal. Moreover, the effect of Sox2 on NPC self-renewal is completely inhibited by AG1478, a specific inhibitor for Egfr; it is also inhibited by LY294002 and U0126, selective antagonists for phosphatidylinositol 3-kinase (PI3K) and extracellular signal-regulated kinase (Erk1/2), respectively. Collectively, we conclude that NPC self-renewal is enhanced through a novel cellular feedback loop with mutual regulation of Egfr and Sox2. STEM CELLS 2010;28:279-286

语种: 英语
所属项目编号: 2006AA02A114 ; D07050701350704 ; 2006CB943603 ; 2006CB503905 ; 2007DFC31650863 ; 30871251 ; 30960150
项目资助者: Chinese National 863 Projects ; Beijing Ministry of Science and Technology ; Chinese National 973 Projects ; Ministry of Science and Technology for International Collaboration Project ; National Natural Science Foundation of China
WOS记录号: WOS:000275058400012
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55081
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
The Egf Receptor-Sox2-Egf Receptor Feedback Loop Positively Regulates the Self-Renewal of Neural Precursor Cells.pdf(698KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Stem Cell Res Ctr, Dept Cell Biol, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Natl Ctr Int Res, Beijing 100191, Peoples R China
3.Ningxia Med Sch, Ningxia, Peoples R China

Recommended Citation:
Hu, Qikuan,Zhang, Lirong,Wen, Jinhua,et al. The Egf Receptor-Sox2-Egf Receptor Feedback Loop Positively Regulates the Self-Renewal of Neural Precursor Cells[J]. STEM CELLS,2010,28(2):279-286.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Hu, Qikuan]'s Articles
[Zhang, Lirong]'s Articles
[Wen, Jinhua]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Hu, Qikuan]‘s Articles
[Zhang, Lirong]‘s Articles
[Wen, Jinhua]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace