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学科主题: 药学
题名:
Simultaneous separation, identification and activity evaluation of three butyrylcholinesterase inhibitors from Plumula nelumbinis using on-line HPLC-UV coupled with ESI-IT-TOF-MS and BChE biochemical detection
作者: Lin, Zongtao1; Wang, Hong1; Fu, Qingrong1; An, Haijuan1; Liang, Yi1; Zhang, Baobao1; Hashi, Yuki2; Chen, Shizhong1
关键词: Butyrylcholinesterase inhibitor ; Biochemical detection ; Plumula nelumbinis ; Alkaloid ; HPLC-UV-ESI-MS-BChEBCD ; IT-TOF
刊名: TALANTA
发表日期: 2013-06-15
DOI: 10.1016/j.talanta.2013.02.033
卷: 110, 页:180-189
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; ACETYLCHOLINESTERASE INHIBITORS ; THEORETICAL CONCEPTS ; DETECTION SYSTEMS ; AGGREGATION ; DISEASE
英文摘要:

We have firstly established a method of high-performance liquid chromatography ultraviolet analysis coupled with electrospray ionization-ion trap-time-of-flight mass spectrometry and butyrylcholinesterase biochemical detection (HPLC-UV-ESI-IT-TOF-MS-BChEBCD). Applying this on-line method to the identification of BChE inhibitors in a Plumula nelumbinis sample, three alkaloids, namely liensinine, isoliensinine, and neferine, have been detected as having a strong BChE inhibition activity for the first time; in addition, norisoliensinine and 6-hydroxynorisoliensinine were proposed as two new compounds identified by their UV and MS data. The HPLC fingerprint, the MS fragments of the components, and the BChE activity profile could be simultaneously recorded during real-time analysis of complex samples using this on-line approach. Tacrine, a BChE inhibitor, was used as a positive reference compound, and its detection limit in the biochemical detection system was 1 nmol. The BChE activity of 1 g of P. nelumbinis sample was equal to that of 127.88 mu mol tacrine. The proposed on-line method has been validated as having good precision and reproducibility, and could be used to rapidly identify BChE inhibitors and to screen potential drugs for the treatment of Alzheimer′s disease in complicated samples. (C) 2013 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2009ZX09102-146
项目资助者: National Science and Technology on New Drug Creation and Development ; Shimadzu International Trading (Shanghai) Co. Limited
WOS记录号: WOS:000319089500026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55097
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Shimadzu Int Trading Shanghai Co Ltd, Beijing 100020, Peoples R China

Recommended Citation:
Lin, Zongtao,Wang, Hong,Fu, Qingrong,et al. Simultaneous separation, identification and activity evaluation of three butyrylcholinesterase inhibitors from Plumula nelumbinis using on-line HPLC-UV coupled with ESI-IT-TOF-MS and BChE biochemical detection[J]. TALANTA,2013,110:180-189.
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