北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 消化内科  > 期刊论文
学科主题: 临床医学
题名:
Changes of CDS+CD28- T regulatory cells in rat model of colitis induced by 2,4-dinitrofluorobenzene
作者: Xiao, WB; Liu, YL
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2003-11-01
卷: 9, 期:11, 页:2528-2532
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: INFLAMMATORY-BOWEL-DISEASE ; ULCERATIVE-COLITIS ; LAMINA PROPRIA ; CROHNS-DISEASE ; INTRAEPITHELIAL LYMPHOCYTES ; INTESTINAL INFLAMMATION ; PERIPHERAL-BLOOD ; MURINE MODEL ; SUBSET ; SUBPOPULATIONS
英文摘要:

AIM: To determine the changes of CD8+ T subsets especially CD8+CD28- T regulatory cells in rat model of experimental colitis induced by 2,4-dinitrofluorobenzene (DNFB).

METHODS: The rat model of experimental colitis was induced by enema with DNFB. Ten days later, colonic intraepithelial and splenic lymphocytes were isolated from colitis animals (n=16) and controls (n=8). The proportion of CD8+ T cells, CD8+CD28+ T cells and CD8+CD28- T regulatory cells were determined by flow cytometry.

RESULTS: The model of experimental colitis was successfully established by DNFB that was demonstrated by bloody diarrhea, weight loss and colonic histopathology. The proportion of CD8+ T cells in either splenic or colonic intraepithelial lymphocytes was not significantly different between colitis animals and controls (spleen: 34.6+/-7.24 % vs 33.5+/-9.41 %, colon: 14.0+/-8.93 % vs 18.0+/-4.06 %, P>0.05). But CD8+CD28- regulatory cells from colitis animals were significantly more than those from controls (spleen: 11.3+/-2.26 % vs 5.64+/-1.01 %, colon: 6.50+/-5.37 % vs 1.07+/-0.65 %, P<0.05). In contrast, CD8+CD28+ T cells from colitis animals were less than those from controls (spleen: 23.3+/-6.14 % vs 27.8+/-9.70 %, P=0.06; colon: 7.52+/-4.18 % vs 16.9+/-4.07 %, P<0.05). The proportion of CD8+CD28- T regulatory cells in splenic and colon intraepithelial CD8+ T cells from colitis animals was higher than that from controls (spleen: 33.3+/-5.49 % vs 18.4+/-7.26 %, colon: 46.0+/-14.3 % vs 6.10+/-3.72 %, P<0.005).

CONCLUSION: Experimental colitis of rats can be induced by DNFB with simplicity and good reproducibility. The proportion of CD8+CD28- T regulatory cells in rats with experimental colitis is increased, which may be associated with the pathogenesis of colitis.

语种: 英语
WOS记录号: WOS:000186761400030
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55128
Appears in Collections:北京大学第二临床医学院_消化内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Peoples Hosp, Dept Gastroenterol, Beijing 100044, Peoples R China

Recommended Citation:
Xiao, WB,Liu, YL. Changes of CDS+CD28- T regulatory cells in rat model of colitis induced by 2,4-dinitrofluorobenzene[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2003,9(11):2528-2532.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xiao, WB]'s Articles
[Liu, YL]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xiao, WB]‘s Articles
[Liu, YL]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace