|Changes of CDS+CD28- T regulatory cells in rat model of colitis induced by 2,4-dinitrofluorobenzene|
|Xiao, WB; Liu, YL|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||INFLAMMATORY-BOWEL-DISEASE ; ULCERATIVE-COLITIS ; LAMINA PROPRIA ; CROHNS-DISEASE ; INTRAEPITHELIAL LYMPHOCYTES ; INTESTINAL INFLAMMATION ; PERIPHERAL-BLOOD ; MURINE MODEL ; SUBSET ; SUBPOPULATIONS|
AIM: To determine the changes of CD8+ T subsets especially CD8+CD28- T regulatory cells in rat model of experimental colitis induced by 2,4-dinitrofluorobenzene (DNFB).
METHODS: The rat model of experimental colitis was induced by enema with DNFB. Ten days later, colonic intraepithelial and splenic lymphocytes were isolated from colitis animals (n=16) and controls (n=8). The proportion of CD8+ T cells, CD8+CD28+ T cells and CD8+CD28- T regulatory cells were determined by flow cytometry.
RESULTS: The model of experimental colitis was successfully established by DNFB that was demonstrated by bloody diarrhea, weight loss and colonic histopathology. The proportion of CD8+ T cells in either splenic or colonic intraepithelial lymphocytes was not significantly different between colitis animals and controls (spleen: 34.6+/-7.24 % vs 33.5+/-9.41 %, colon: 14.0+/-8.93 % vs 18.0+/-4.06 %, P>0.05). But CD8+CD28- regulatory cells from colitis animals were significantly more than those from controls (spleen: 11.3+/-2.26 % vs 5.64+/-1.01 %, colon: 6.50+/-5.37 % vs 1.07+/-0.65 %, P<0.05). In contrast, CD8+CD28+ T cells from colitis animals were less than those from controls (spleen: 23.3+/-6.14 % vs 27.8+/-9.70 %, P=0.06; colon: 7.52+/-4.18 % vs 16.9+/-4.07 %, P<0.05). The proportion of CD8+CD28- T regulatory cells in splenic and colon intraepithelial CD8+ T cells from colitis animals was higher than that from controls (spleen: 33.3+/-5.49 % vs 18.4+/-7.26 %, colon: 46.0+/-14.3 % vs 6.10+/-3.72 %, P<0.005).
CONCLUSION: Experimental colitis of rats can be induced by DNFB with simplicity and good reproducibility. The proportion of CD8+CD28- T regulatory cells in rats with experimental colitis is increased, which may be associated with the pathogenesis of colitis.
|作者单位||Peking Univ, Peoples Hosp, Dept Gastroenterol, Beijing 100044, Peoples R China|
|Xiao, WB,Liu, YL. Changes of CDS+CD28- T regulatory cells in rat model of colitis induced by 2,4-dinitrofluorobenzene[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2003,9(11):2528-2532.|
|APA||Xiao, WB,&Liu, YL.(2003).Changes of CDS+CD28- T regulatory cells in rat model of colitis induced by 2,4-dinitrofluorobenzene.WORLD JOURNAL OF GASTROENTEROLOGY,9(11),2528-2532.|
|MLA||Xiao, WB,et al."Changes of CDS+CD28- T regulatory cells in rat model of colitis induced by 2,4-dinitrofluorobenzene".WORLD JOURNAL OF GASTROENTEROLOGY 9.11(2003):2528-2532.|