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学科主题: 临床医学
题名:
EPLIN-alpha Expression in Human Oesophageal Cancer and its Impact on Cellular Aggressiveness and Clinical Outcome
作者: Liu, Yinan1,2; Sanders, Andrew J.1; Zhang, Lijian2; Jiang, Wen G.1
关键词: EPLIN alpha ; oesophageal cancer ; ECIS ; cell migration ; KYSE 150 cells ; forced expression
刊名: ANTICANCER RESEARCH
发表日期: 2012-04-01
卷: 32, 期:4, 页:1283-1289
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: EPITHELIAL PROTEIN LOST ; CROSS-LINKING ; ACTIN ; NEOPLASM ; CELLS
英文摘要:

Background: Epithelial protein lost in neoplasm-alpha (EPLIN-alpha) is a cytoskeletal protein whose expression is often lost or is aberrant in cancerous cells and tissues and whose loss is believed to be involved in aggressive phenotype. Our current study examined this molecule in human oesophageal tissues and investigated the cellular impact of EPLIN-alpha on oesophageal cancer cells. Materials and Methods: Expression of the EPLIN-alpha transcript in human oesophageal tissues (tumour, paratumour and normal) was determined using the Quantitative Polymerase Chain Reaction (Q-PCR) method. In vitro models, including invasion, cellular migration (Electrical Cell substrate Impedance Sensing based method), cell growth and matrix adhesion assays were employed in order to assess the biological influence of EPLIN-alpha expression on KYSE150 oesophageal cancer cells. Results: EPLIN-a expression was lower in tumour tissues compared to normal tissue. Grade 3-5 tumours had slightly lower levels of EPLIN-alpha compared with those of grade 2. Patients who died of oesophageal cancer had significantly lower levels of EPLIN-a compared to those who remained disease-free (p=0.022). Lower levels of EPLIN-alpha transcript were seen in advanced oesophageal cancer, including TNM stages 2 to 4. Reduced EPLIN-alpha expression was associated with lymphatic metastasis and local advanced T-stage cancel; including T2-T4. Forced expression of EPLIN-alpha in oesophageal cancer cells rendered cells less invasive and reduced their cell growth rate in vitro. Conclusion: Our study suggests that EPLIN-alpha is expressed at lower levels in oesophageal cancer tissues. This down-regulation has a prognostic value. Together with the findings that EPLIN-alpha inhibits cellular growth and invasion, we conclude that EPLIN-alpha is a tumour suppressor of oesophageal cancer.

语种: 英语
项目资助者: Cardiff University ; Cancer Research Wales
WOS记录号: WOS:000302492600020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55133
Appears in Collections:北京大学临床肿瘤学院_胸外科_期刊论文

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作者单位: 1.Cardiff Univ, Sch Med, Metastasis & Angiogenesis Res Grp, Cardiff CF14 4XN, S Glam, Wales
2.Peking Univ, Canc Hosp & Inst, Dept Thorac Surg 2, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China

Recommended Citation:
Liu, Yinan,Sanders, Andrew J.,Zhang, Lijian,et al. EPLIN-alpha Expression in Human Oesophageal Cancer and its Impact on Cellular Aggressiveness and Clinical Outcome[J]. ANTICANCER RESEARCH,2012,32(4):1283-1289.
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