IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Cytoskeletal and nuclear organization in mouse embryos derived from nuclear transfer and ICSI: A comparison of agamogony and syngamy before and during the first cell cycle
Zhu, Zi-Yu; Jiang, Man-Xi; Yan, Li-Ying; Huang, Jun-Cheng; Lei, Zi-Li; Jiang, Yan; Ouyang, Ying-Chun; Zhang, Huan-Xiang; Sun, Qing-Yuan; Chen, Da-Yuan
关键词Nuclear Transfer Icsi Microtubule Microfilament Oocyte Mouse
刊名MOLECULAR REPRODUCTION AND DEVELOPMENT
2007-05-01
DOI10.1002/mrd.20646
74期:5页:655-663
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Developmental Biology ; Reproductive Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Developmental Biology ; Reproductive Biology
关键词[WOS]GERMINAL VESICLE ; MEIOTIC SPINDLE ; CLONED EMBRYOS ; SOMATIC-CELLS ; OOCYTES ; CLONING ; MICE ; CENTROSOME ; SEQUENCES ; PROTEIN
英文摘要

In this study, somatic cell nuclear transfer (SCNT) and intracytoplasmic sperm injection (ICSI) are used as models of agamogony and syngamy, respectively. In order to elucidate the reasons of low efficiency of somatic cell cloning, cytoskeletal and nuclear organization in cloned mouse embryos was monitored before and during the first cell cycle, and compared with the pattern of ICSI zygote. A metaphase-like spindle with alignment of condensed donor chromosomes was assembled within 3 hr after NT, followed by formation of pronuclear-like structures at 3-6 hr after activation, indicating that somatic nuclear remodeling depends on microtubular network organization. The percentage of two (pseudo-) pronuclei in cloned embryos derived from delayed activation was greater than that in immediate activation group (68.5% vs. 30.8%, P < 0.01), but similar to that of ICSI group (68.5% vs. 65.5%, P > 0.05). The 2-cell rate in NT embryos was significantly lower than that in zygotes produced by ICSI (64.8% vs. 82.5%, P < 0.01). Further studies testified that the cloned embryos reached the metaphase of the first mitosis 10 hr after activation, whereas this occurred at 18 hr in the ICSI zygotes. Comparision of the pattern of microfilament assembly in early NT embryos with that in syngamic zygotes suggested that abnormal microfilamental pattern in cloned embryos may threaten subsequent embryonic development. In conclusion, agamogony, in contrast to syngamy, displays some unique features in respect of cytoskeletal organization, the most remarkable of which is that the first cell cycle is initiated ahead distinctly, which probably leads to incomplete organization of the first mitotic spindle, and contributes to low efficiency of cloning.

语种英语
WOS记录号WOS:000245044300015
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55136
Collection北京大学第三临床医学院
作者单位1.Soochow Univ, Coll Life Sci, Suzhou, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peoples R China
3.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Zi-Yu,Jiang, Man-Xi,Yan, Li-Ying,et al. Cytoskeletal and nuclear organization in mouse embryos derived from nuclear transfer and ICSI: A comparison of agamogony and syngamy before and during the first cell cycle[J]. MOLECULAR REPRODUCTION AND DEVELOPMENT,2007,74(5):655-663.
APA Zhu, Zi-Yu.,Jiang, Man-Xi.,Yan, Li-Ying.,Huang, Jun-Cheng.,Lei, Zi-Li.,...&Chen, Da-Yuan.(2007).Cytoskeletal and nuclear organization in mouse embryos derived from nuclear transfer and ICSI: A comparison of agamogony and syngamy before and during the first cell cycle.MOLECULAR REPRODUCTION AND DEVELOPMENT,74(5),655-663.
MLA Zhu, Zi-Yu,et al."Cytoskeletal and nuclear organization in mouse embryos derived from nuclear transfer and ICSI: A comparison of agamogony and syngamy before and during the first cell cycle".MOLECULAR REPRODUCTION AND DEVELOPMENT 74.5(2007):655-663.
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