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学科主题: 基础医学
题名:
A tetracycline-regulatable adeno-associated virus vector for double-gene transfer
作者: Wang, JJ; Niu, DB; Zhang, T; Wang, K; Xue, B; Wang, XM
关键词: tetracycline-regulatable ; adeno-associated virus ; PC12 cells ; green fluorescent protein
刊名: NEUROSCIENCE LETTERS
发表日期: 2005-04-18
DOI: 10.1016/j.neulet.2004.12.039
卷: 378, 期:2, 页:106-110
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: NEUROTROPHIC FACTOR ; PARKINSONS-DISEASE ; EXPRESSION ; BRAIN ; DELIVERY ; CELLS
英文摘要:

An increasing demand for polycistronic vectors that express multiple genes simultaneously has arisen in recent years to obtain an efficient gene therapy. Armed with the knowledge that the expression of transgene in mammalian cells often requires tight control, we constructed in this study a tetracycline-regulated double-gene adeno-associated virus (AAV) vector carrying green and red fluorescent protein genes and expressed it in PC 12 cells. When detected by fluorescence microscope and fluorescence-activated cell sorting, gene expression was induced by 44-66-fold and could be reversibly controlled by doxycycline. This double-gene AAV vector may be useful for regulated expression of two genes or a marker to monitor transgene expression. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000228083300009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55137
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Capital Univ Med Sci, Dept Physiol, Beijing 100054, Peoples R China

Recommended Citation:
Wang, JJ,Niu, DB,Zhang, T,et al. A tetracycline-regulatable adeno-associated virus vector for double-gene transfer[J]. NEUROSCIENCE LETTERS,2005,378(2):106-110.
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