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学科主题基础医学
A tetracycline-regulatable adeno-associated virus vector for double-gene transfer
Wang, JJ; Niu, DB; Zhang, T; Wang, K; Xue, B; Wang, XM
关键词tetracycline-regulatable adeno-associated virus PC12 cells green fluorescent protein
刊名NEUROSCIENCE LETTERS
2005-04-18
DOI10.1016/j.neulet.2004.12.039
378期:2页:106-110
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]NEUROTROPHIC FACTOR ; PARKINSONS-DISEASE ; EXPRESSION ; BRAIN ; DELIVERY ; CELLS
英文摘要

An increasing demand for polycistronic vectors that express multiple genes simultaneously has arisen in recent years to obtain an efficient gene therapy. Armed with the knowledge that the expression of transgene in mammalian cells often requires tight control, we constructed in this study a tetracycline-regulated double-gene adeno-associated virus (AAV) vector carrying green and red fluorescent protein genes and expressed it in PC 12 cells. When detected by fluorescence microscope and fluorescence-activated cell sorting, gene expression was induced by 44-66-fold and could be reversibly controlled by doxycycline. This double-gene AAV vector may be useful for regulated expression of two genes or a marker to monitor transgene expression. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000228083300009
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55137
专题北京大学基础医学院_神经生物学系
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Capital Univ Med Sci, Dept Physiol, Beijing 100054, Peoples R China
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GB/T 7714
Wang, JJ,Niu, DB,Zhang, T,et al. A tetracycline-regulatable adeno-associated virus vector for double-gene transfer[J]. NEUROSCIENCE LETTERS,2005,378(2):106-110.
APA Wang, JJ,Niu, DB,Zhang, T,Wang, K,Xue, B,&Wang, XM.(2005).A tetracycline-regulatable adeno-associated virus vector for double-gene transfer.NEUROSCIENCE LETTERS,378(2),106-110.
MLA Wang, JJ,et al."A tetracycline-regulatable adeno-associated virus vector for double-gene transfer".NEUROSCIENCE LETTERS 378.2(2005):106-110.
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