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学科主题: 基础医学
题名:
Tumorigenesis of murine erythroleukemia cell line transfected with exogenous p53 gene
作者: Sun, DG; Wang, JH; Yao, WJ; Gu, L; Wen, ZY; Shu, C
关键词: p53 gene ; murine erythroleukemia cells ; tumorigenesis ; gene transfection
刊名: CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
发表日期: 2004
卷: 30, 期:2, 页:117-126
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology ; Peripheral Vascular Disease
研究领域[WOS]: Hematology ; Cardiovascular System & Cardiology
关键词[WOS]: ERYTHROCYTE DEFORMABILITY ; LOW-VISCOSITY ; RBC MEMBRANE ; EKTACYTOMETRY ; APOPTOSIS ; SEPSIS
英文摘要:

Mutant p53 gene and wild-type p53 gene were introduced into murine erythroleukemia cell line (MEL). The MEL cells transfected with mutant p53 gene (MEL-M) and with wild-type p53 gene (MEL-W) were obtained by G418 selection. MEL, MEL-W and MEL-M were injected intraperitoneally into BALB/C mice. In the first week after injection, the signs of erythroleukemia were induced in all three groups. Abnormalities were mainly found in the spleen, bone marrow, liver and peripheral blood. There was an increase of proerythroblasts in the bone marrow. A large amount of normoblasts (early and intermediate erythroblasts) appeared in the spleen. In the peripheral blood, the white blood cells, reticulocyte and platelet counts increased and RBC count and hematocrit decreased. The degree of abnormalities in the MEL-W group was significantly lower than that in other two groups. Hemorheological measurements indicated that the deformability and orientation of RBCs in MEL and MEL-M groups were impaired, whereas those in MEL-W group did not change significantly. Micropipette aspiration measurement revealed that MEL-W had higher elastic modulus than MEL and MEL-M, indicating that it was more difficult for MEL-W to deform and migrate in vivo. The results of animal test and micropipette suggest that exogenous wild-type p53 gene could reduce the tumorigenesis of murine erythroleukemia cells.

语种: 英语
WOS记录号: WOS:000220566900005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55148
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Dept Med Phys, Hemorheol Ctr, Hlth Sci Ctr,Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA

Recommended Citation:
Sun, DG,Wang, JH,Yao, WJ,et al. Tumorigenesis of murine erythroleukemia cell line transfected with exogenous p53 gene[J]. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,2004,30(2):117-126.
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