IR@PKUHSC  > 北京大学基础医学院  > 生物物理学系
学科主题基础医学
Tumorigenesis of murine erythroleukemia cell line transfected with exogenous p53 gene
Sun, DG; Wang, JH; Yao, WJ; Gu, L; Wen, ZY; Shu, C
关键词P53 Gene Murine Erythroleukemia Cells Tumorigenesis Gene Transfection
刊名CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
2004
30期:2页:117-126
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Hematology ; Peripheral Vascular Disease
研究领域[WOS]Hematology ; Cardiovascular System & Cardiology
关键词[WOS]ERYTHROCYTE DEFORMABILITY ; LOW-VISCOSITY ; RBC MEMBRANE ; EKTACYTOMETRY ; APOPTOSIS ; SEPSIS
英文摘要

Mutant p53 gene and wild-type p53 gene were introduced into murine erythroleukemia cell line (MEL). The MEL cells transfected with mutant p53 gene (MEL-M) and with wild-type p53 gene (MEL-W) were obtained by G418 selection. MEL, MEL-W and MEL-M were injected intraperitoneally into BALB/C mice. In the first week after injection, the signs of erythroleukemia were induced in all three groups. Abnormalities were mainly found in the spleen, bone marrow, liver and peripheral blood. There was an increase of proerythroblasts in the bone marrow. A large amount of normoblasts (early and intermediate erythroblasts) appeared in the spleen. In the peripheral blood, the white blood cells, reticulocyte and platelet counts increased and RBC count and hematocrit decreased. The degree of abnormalities in the MEL-W group was significantly lower than that in other two groups. Hemorheological measurements indicated that the deformability and orientation of RBCs in MEL and MEL-M groups were impaired, whereas those in MEL-W group did not change significantly. Micropipette aspiration measurement revealed that MEL-W had higher elastic modulus than MEL and MEL-M, indicating that it was more difficult for MEL-W to deform and migrate in vivo. The results of animal test and micropipette suggest that exogenous wild-type p53 gene could reduce the tumorigenesis of murine erythroleukemia cells.

语种英语
WOS记录号WOS:000220566900005
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55148
专题北京大学基础医学院_生物物理学系
作者单位1.Peking Univ, Dept Med Phys, Hemorheol Ctr, Hlth Sci Ctr,Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
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GB/T 7714
Sun, DG,Wang, JH,Yao, WJ,et al. Tumorigenesis of murine erythroleukemia cell line transfected with exogenous p53 gene[J]. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,2004,30(2):117-126.
APA Sun, DG,Wang, JH,Yao, WJ,Gu, L,Wen, ZY,&Shu, C.(2004).Tumorigenesis of murine erythroleukemia cell line transfected with exogenous p53 gene.CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,30(2),117-126.
MLA Sun, DG,et al."Tumorigenesis of murine erythroleukemia cell line transfected with exogenous p53 gene".CLINICAL HEMORHEOLOGY AND MICROCIRCULATION 30.2(2004):117-126.
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