IR@PKUHSC  > 北京大学医药卫生分析中心
学科主题公共卫生
Optical Imaging of Integrin alpha(v)beta(3) Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers
Liu, Zhaofei; Liu, Shuanglong; Niu, Gang; Wang, Fan; Liu, Shuang; Chen, Xiaoyuan1
刊名MOLECULAR IMAGING
2010
DOI10.2310/7290.2009.00032
9期:1页:21-29
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]Biochemistry & Molecular Biology ; Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]TUMOR ANGIOGENESIS ; CONTRAST AGENTS ; CANCER-THERAPY ; PEPTIDES ; PET ; TOMOGRAPHY
英文摘要

Integrin alpha(v)beta(3) plays great roles in tumor angiogenesis, invasion, and metastasis. We report here the noninvasive visualization of tumor integrin alpha(v)beta(3) expression by using near-infrared fluorescence (NIRF) imaging of an IRDye800-labeled new cyclic RGD (arginine-glycine-aspartic acid) dimer with tetra(ethylene glycol) (PEG(4)) linkers (ie, E[PEG(4)-c(RGDfK)](2), PEG(4) = 15-amino-4,7,10,13-tetraoxapentadecanoic acid) in a U87MG tumor model. Fluorescent dye-labeled E[PEG(4)-c(RGDfK)](2) were subjected to in vitro cell staining, in vivo NIRF imaging, ex vivo NIRF imaging, and histologic studies. The in vitro and in vivo characterization of dye-labeled E[PEG(4)-c(RGDfK)](2) were compared with dye-labeled RGD dimer without PEG(4) linkers (namely, E[c(RGDfK)](2)). Both Cy5.5-E[PEG(4)-c(RGDfK)](2) and Cy5.5-E[c(RGDfK)](2) exhibited integrin alpha(v)beta(3) binding specificity in a cell-staining experiment. In vivo NIRF imaging showed higher tumor accumulation and tumor to background contrast of IRDye800-E[PEG(4)-c(RGDfK)](2) over IRDye800-E[c(RGDfK)](2). The tumor integrin alpha(v)beta(3) specificity of IRDye800-E[PEG(4)-c(RGDfK)](2) was confirmed by successful inhibition of tumor uptake in the presence of an excess dose of c(RGDfK). Histologic examination revealed both tumor vasculature and tumor cell integrin alpha(v)beta(3) binding of IRDye800-E[PEG(4)-c(RGDfK)](2) in vivo. In summary, NIRF imaging with IRDye800-E[PEG(4)-c(RGDfK)](2) offers an easy, fast, and low-cost way to detect and semiquantify tumor integrin alpha(v)beta(3) expression in living subjects.

语种英语
WOS记录号WOS:000274228900002
项目编号R01 120188 ; R01 CA119053 ; R21 CA121842 ; R21 CA102123 ; P50 CA114747 ; U54 CA119367 ; R24 CA93862 ; R01 CA115883 ; DE-FG02-08ER64684
资助机构National Cancer Institute ; Department of Energy ; China Scholarship Council
引用统计
被引频次:32[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55149
专题北京大学医药卫生分析中心
作者单位1.Stanford Univ, Sch Med, Bio X Program, Stanford, CA 94305 USA
2.NIBIB, Lab Mol Imaging & Nanomed, CC, NIH, Bethesda, MD 20892 USA
3.Stanford Univ, Sch Med, Mol Imaging Program Stanford, Dept Radiol, Stanford, CA 94305 USA
4.Stanford Univ, Sch Med, Mol Imaging Program Stanford, Dept Biophys, Stanford, CA 94305 USA
5.Peking Univ, Med Isotopes Res Ctr, Beijing 100871, Peoples R China
6.Purdue Univ, Sch Hlth Sci, W Lafayette, IN 47907 USA
推荐引用方式
GB/T 7714
Liu, Zhaofei,Liu, Shuanglong,Niu, Gang,et al. Optical Imaging of Integrin alpha(v)beta(3) Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers[J]. MOLECULAR IMAGING,2010,9(1):21-29.
APA Liu, Zhaofei,Liu, Shuanglong,Niu, Gang,Wang, Fan,Liu, Shuang,&Chen, Xiaoyuan.(2010).Optical Imaging of Integrin alpha(v)beta(3) Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers.MOLECULAR IMAGING,9(1),21-29.
MLA Liu, Zhaofei,et al."Optical Imaging of Integrin alpha(v)beta(3) Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers".MOLECULAR IMAGING 9.1(2010):21-29.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Zhaofei]的文章
[Liu, Shuanglong]的文章
[Niu, Gang]的文章
百度学术
百度学术中相似的文章
[Liu, Zhaofei]的文章
[Liu, Shuanglong]的文章
[Niu, Gang]的文章
必应学术
必应学术中相似的文章
[Liu, Zhaofei]的文章
[Liu, Shuanglong]的文章
[Niu, Gang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。