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学科主题: 公共卫生
题名:
Optical Imaging of Integrin alpha(v)beta(3) Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers
作者: Liu, Zhaofei; Liu, Shuanglong; Niu, Gang; Wang, Fan; Liu, Shuang; Chen, Xiaoyuan1
刊名: MOLECULAR IMAGING
发表日期: 2010
DOI: 10.2310/7290.2009.00032
卷: 9, 期:1, 页:21-29
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]: Biochemistry & Molecular Biology ; Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]: TUMOR ANGIOGENESIS ; CONTRAST AGENTS ; CANCER-THERAPY ; PEPTIDES ; PET ; TOMOGRAPHY
英文摘要:

Integrin alpha(v)beta(3) plays great roles in tumor angiogenesis, invasion, and metastasis. We report here the noninvasive visualization of tumor integrin alpha(v)beta(3) expression by using near-infrared fluorescence (NIRF) imaging of an IRDye800-labeled new cyclic RGD (arginine-glycine-aspartic acid) dimer with tetra(ethylene glycol) (PEG(4)) linkers (ie, E[PEG(4)-c(RGDfK)](2), PEG(4) = 15-amino-4,7,10,13-tetraoxapentadecanoic acid) in a U87MG tumor model. Fluorescent dye-labeled E[PEG(4)-c(RGDfK)](2) were subjected to in vitro cell staining, in vivo NIRF imaging, ex vivo NIRF imaging, and histologic studies. The in vitro and in vivo characterization of dye-labeled E[PEG(4)-c(RGDfK)](2) were compared with dye-labeled RGD dimer without PEG(4) linkers (namely, E[c(RGDfK)](2)). Both Cy5.5-E[PEG(4)-c(RGDfK)](2) and Cy5.5-E[c(RGDfK)](2) exhibited integrin alpha(v)beta(3) binding specificity in a cell-staining experiment. In vivo NIRF imaging showed higher tumor accumulation and tumor to background contrast of IRDye800-E[PEG(4)-c(RGDfK)](2) over IRDye800-E[c(RGDfK)](2). The tumor integrin alpha(v)beta(3) specificity of IRDye800-E[PEG(4)-c(RGDfK)](2) was confirmed by successful inhibition of tumor uptake in the presence of an excess dose of c(RGDfK). Histologic examination revealed both tumor vasculature and tumor cell integrin alpha(v)beta(3) binding of IRDye800-E[PEG(4)-c(RGDfK)](2) in vivo. In summary, NIRF imaging with IRDye800-E[PEG(4)-c(RGDfK)](2) offers an easy, fast, and low-cost way to detect and semiquantify tumor integrin alpha(v)beta(3) expression in living subjects.

语种: 英语
所属项目编号: R01 120188 ; R01 CA119053 ; R21 CA121842 ; R21 CA102123 ; P50 CA114747 ; U54 CA119367 ; R24 CA93862 ; R01 CA115883 ; DE-FG02-08ER64684
项目资助者: National Cancer Institute ; Department of Energy ; China Scholarship Council
WOS记录号: WOS:000274228900002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55149
Appears in Collections:北京大学医药卫生分析中心_期刊论文

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作者单位: 1.Stanford Univ, Sch Med, Bio X Program, Stanford, CA 94305 USA
2.NIBIB, Lab Mol Imaging & Nanomed, CC, NIH, Bethesda, MD 20892 USA
3.Stanford Univ, Sch Med, Mol Imaging Program Stanford, Dept Radiol, Stanford, CA 94305 USA
4.Stanford Univ, Sch Med, Mol Imaging Program Stanford, Dept Biophys, Stanford, CA 94305 USA
5.Peking Univ, Med Isotopes Res Ctr, Beijing 100871, Peoples R China
6.Purdue Univ, Sch Hlth Sci, W Lafayette, IN 47907 USA

Recommended Citation:
Liu, Zhaofei,Liu, Shuanglong,Niu, Gang,et al. Optical Imaging of Integrin alpha(v)beta(3) Expression with Near-Infrared Fluorescent RGD Dimer with Tetra(ethylene glycol) Linkers[J]. MOLECULAR IMAGING,2010,9(1):21-29.
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