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The role of simvastatin in the osteogenesis of injectable tissue-engineered bone based on human adipose-derived stromal cells and platelet-rich plasma
Zhou, Yongsheng1,2,3,4; Ni, Yongwei1,2; Liu, Yunsong1,2; Zeng, Baijin1,2; Xu, Yongwei1,2; Ge, Wenshu1,2
关键词Simvastatin Adipose-derived Stromal Cells Platelet-rich Plasma Bone Tissue Engineering Critical-sized Calvarial Defects Injectable Bone
刊名BIOMATERIALS
2010-07-01
DOI10.1016/j.biomaterials.2010.03.037
31期:20页:5325-5335
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]MESENCHYMAL STEM-CELLS ; OSTEOBLAST DIFFERENTIATION ; DISTRACTION OSTEOGENESIS ; MC3T3-E1 CELLS ; IN-VITRO ; STATINS ; REGENERATION ; EXPRESSION ; FRACTURE ; ANGIOGENESIS
英文摘要

An injectable tissue-engineered bone (ITB) composed of human adipose-derived stromal cells (hADSCs) and platelet-rich plasma (hPRP) was preliminarily constructed, but its osteogenic capability needs improving. This study aimed to evaluate if simvastatin can be applied as a bone anabolic agent for this ITB. We found 0.01 mu m, 0.1 mu m, and 1 mu m simvastatin could induce hADSCs′ osteoblastic differentiation in vitro that accompanied with non-inhibition on cell proliferation, high alkaline phosphatase activity, more mineralization deposition and more expression of osteoblast-related genes such as osteocalcin, core binding factor alpha 1, bone morphogenetic protein-2, vascular endothelial growth factor, and basic fibroblast growth factor. Simvastatin at 1 mu m seemed the most optimal concentration due to its high osteocalcin secretion in media (P < 0.01). Quantitative mineralization assay also showed 1 mu m SIM had the most obvious synergistic effect on hPRP′s induction for matrix mineralization of hADSCs (P < 0.01). When 1 pm Simvastatin was applied to this ITB to restore the critical-sized calvarial defects in mice, more bone formation was observed in defected regions, and the peripheries just outside the defect margins by X-ray analysis, and H&E staining. These findings indicate that simvastatin at optimal concentrations can be used to promote this ITB′s osteogenesis. However, simvastatin′s effects on this ITB await long-term investigation. (C) 2010 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000278571800005
项目编号30200319 ; 30901693
资助机构National Natural Science Foundation of China ; PKU school of stomatology for young scientists
引用统计
被引频次:44[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55150
专题北京大学口腔医学院_口腔修复科
北京大学口腔医学院_综合治疗二科
作者单位1.Peking Univ, Dept Prosthodont, Sch Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Dept Prosthodont, Hosp Stomatol, Beijing 100081, Peoples R China
3.Peking Univ, Core Lab, Sch Stomatol, Beijing 100081, Peoples R China
4.Peking Univ, Core Lab, Hosp Stomatol, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Zhou, Yongsheng,Ni, Yongwei,Liu, Yunsong,et al. The role of simvastatin in the osteogenesis of injectable tissue-engineered bone based on human adipose-derived stromal cells and platelet-rich plasma[J]. BIOMATERIALS,2010,31(20):5325-5335.
APA Zhou, Yongsheng,Ni, Yongwei,Liu, Yunsong,Zeng, Baijin,Xu, Yongwei,&Ge, Wenshu.(2010).The role of simvastatin in the osteogenesis of injectable tissue-engineered bone based on human adipose-derived stromal cells and platelet-rich plasma.BIOMATERIALS,31(20),5325-5335.
MLA Zhou, Yongsheng,et al."The role of simvastatin in the osteogenesis of injectable tissue-engineered bone based on human adipose-derived stromal cells and platelet-rich plasma".BIOMATERIALS 31.20(2010):5325-5335.
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