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学科主题: 口腔医学
题名:
The role of simvastatin in the osteogenesis of injectable tissue-engineered bone based on human adipose-derived stromal cells and platelet-rich plasma
作者: Zhou, Yongsheng1,2,3,4; Ni, Yongwei1,2; Liu, Yunsong1,2; Zeng, Baijin1,2; Xu, Yongwei1,2; Ge, Wenshu1,2
关键词: Simvastatin ; Adipose-derived stromal cells ; Platelet-rich plasma ; Bone tissue engineering ; Critical-sized calvarial defects ; Injectable bone
刊名: BIOMATERIALS
发表日期: 2010-07-01
DOI: 10.1016/j.biomaterials.2010.03.037
卷: 31, 期:20, 页:5325-5335
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: MESENCHYMAL STEM-CELLS ; OSTEOBLAST DIFFERENTIATION ; DISTRACTION OSTEOGENESIS ; MC3T3-E1 CELLS ; IN-VITRO ; STATINS ; REGENERATION ; EXPRESSION ; FRACTURE ; ANGIOGENESIS
英文摘要:

An injectable tissue-engineered bone (ITB) composed of human adipose-derived stromal cells (hADSCs) and platelet-rich plasma (hPRP) was preliminarily constructed, but its osteogenic capability needs improving. This study aimed to evaluate if simvastatin can be applied as a bone anabolic agent for this ITB. We found 0.01 mu m, 0.1 mu m, and 1 mu m simvastatin could induce hADSCs′ osteoblastic differentiation in vitro that accompanied with non-inhibition on cell proliferation, high alkaline phosphatase activity, more mineralization deposition and more expression of osteoblast-related genes such as osteocalcin, core binding factor alpha 1, bone morphogenetic protein-2, vascular endothelial growth factor, and basic fibroblast growth factor. Simvastatin at 1 mu m seemed the most optimal concentration due to its high osteocalcin secretion in media (P < 0.01). Quantitative mineralization assay also showed 1 mu m SIM had the most obvious synergistic effect on hPRP′s induction for matrix mineralization of hADSCs (P < 0.01). When 1 pm Simvastatin was applied to this ITB to restore the critical-sized calvarial defects in mice, more bone formation was observed in defected regions, and the peripheries just outside the defect margins by X-ray analysis, and H&E staining. These findings indicate that simvastatin at optimal concentrations can be used to promote this ITB′s osteogenesis. However, simvastatin′s effects on this ITB await long-term investigation. (C) 2010 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 30200319 ; 30901693
项目资助者: National Natural Science Foundation of China ; PKU school of stomatology for young scientists
WOS记录号: WOS:000278571800005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55150
Appears in Collections:北京大学口腔医学院_口腔修复科_期刊论文

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作者单位: 1.Peking Univ, Dept Prosthodont, Sch Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Dept Prosthodont, Hosp Stomatol, Beijing 100081, Peoples R China
3.Peking Univ, Core Lab, Sch Stomatol, Beijing 100081, Peoples R China
4.Peking Univ, Core Lab, Hosp Stomatol, Beijing 100081, Peoples R China

Recommended Citation:
Zhou, Yongsheng,Ni, Yongwei,Liu, Yunsong,et al. The role of simvastatin in the osteogenesis of injectable tissue-engineered bone based on human adipose-derived stromal cells and platelet-rich plasma[J]. BIOMATERIALS,2010,31(20):5325-5335.
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