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Involvement of NOX in the Regulation of Renal Tubular Expression of Na/K-ATPase in Acute Unilateral Ureteral Obstruction Rats
Liu, Changxuan1,2; Song, Yi3; Qu, Lei1,2; Tang, Jiawei1,2; Meng, Liqiang1,2; Wang, Yu1,2
关键词Na/k-atpase Nadph Oxidase Oxidative Stress Unilateral Ureteral Obstruction
刊名NEPHRON
2015
DOI10.1159/000381858
130期:1页:66-76
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]OXIDATIVE STRESS ; NADPH OXIDASE ; RENOVASCULAR HYPERTENSION ; DIABETIC-NEPHROPATHY ; DOWN-REGULATION ; NA+/K+-ATPASE ; KIDNEY ; APOCYNIN ; INJURY ; CELLS
英文摘要

Background/Aims:Renal tubular expression of Na/K-ATPase has been reported to be rapidly reduced in kidneys after unilateral ureteral obstruction (UUO), contributing to compromised sodium regulation. In this study, apocynin was utilized to test the hypothesis that the renal nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) system is involved in the regulation of Na/K-ATPase expression in early UUO. Methods: Eighty Wistar rats underwent UUO or sham surgery, and half of each group was administered 100 mg/kg apocynin by oral gavage after surgery. Renal tissue samples were collected on day 1 and day 3 after surgery and the distribution of NOX2, NOX4 and Na/K-ATPase was assessed by immunofluorescence and immunohistochemical staining. The Heme oxygenase-1 (HO-1), NOX2, NOX4, osteopontin, Na/K-ATPase and aquaporin-1 (AQP-1) content of renal homogenates was assessed by immunoblotting, and malondialdehyde (MDA), nitric oxide (NO) content was assessed by biochemical analyses. Activity of inducible NO synthase (iNOS) and antioxidant enzymes was assessed by enzymatic assay. Results: Kidney Na/K-ATPase and AQP-1 content was decreased, and MDA and NOX4, NOX2 and HO-1 content were increased in the obstructed kidneys of UUO rats. Apocynin attenuated these changes and partially, but significantly, reversed the downregulation of Na/K-ATPase and AQP-1 in UUO rats. However, apocynin did not alter iNOS activity and NO production, osteopontin expression or the activity of antioxidant enzymes. Conclusion: Activation of the NOX system and subsequent production of ROS are likely responsible for the downregulation of renal tubular Na/K-ATPase expression in early UUO. (C) 2015 S. Karger AG, Basel

语种英语
WOS记录号WOS:000355772500011
项目编号81041001 ; 81270796
资助机构Chinese National Science Foundation ; Research Foundation of Peking University First Hospital
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55158
专题北京大学第一临床医学院_肾脏内科
作者单位1.Peking Univ, Hosp 1, Dept Med, Div Renal, Beijing 100034, Peoples R China
2.Peking Univ, Key Lab Renal Dis, Key Lab Chron Kidney Dis Prevent & Treatment, Inst Nephrol,Minist Hlth China, Beijing 100034, Peoples R China
3.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
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GB/T 7714
Liu, Changxuan,Song, Yi,Qu, Lei,et al. Involvement of NOX in the Regulation of Renal Tubular Expression of Na/K-ATPase in Acute Unilateral Ureteral Obstruction Rats[J]. NEPHRON,2015,130(1):66-76.
APA Liu, Changxuan,Song, Yi,Qu, Lei,Tang, Jiawei,Meng, Liqiang,&Wang, Yu.(2015).Involvement of NOX in the Regulation of Renal Tubular Expression of Na/K-ATPase in Acute Unilateral Ureteral Obstruction Rats.NEPHRON,130(1),66-76.
MLA Liu, Changxuan,et al."Involvement of NOX in the Regulation of Renal Tubular Expression of Na/K-ATPase in Acute Unilateral Ureteral Obstruction Rats".NEPHRON 130.1(2015):66-76.
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