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学科主题基础医学
Novel multifunctional chitosan-GMA-IDA-Cu(II) nanospheres for high dynamic range characterization of the human plasma proteome
Zou, Xiajuan1; Zhong, Lijun1; Liu, Dan1; Yang, Bin1; Lou, Yaxin1; Peng, Jiarou1; Rainer, Matthias3; Feuerstein, Isabel3; Muhammad, Najam-ul-Haq3; Huck, Christian W.3; Bonn, Guenther K.3; Yin, Yuxin1,2
关键词Plasma Nanosphere Biomarkers Chitosan-gma-ida-cu(Ii) Maldi-tof-ms
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2011-05-01
DOI10.1007/s00216-011-4812-y
400期:3页:747-756
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]HUMAN SERUM PROTEOME ; MASS-SPECTROMETRY ; 2-DIMENSIONAL ELECTROPHORESIS ; CANCER BIOMARKERS ; OVARIAN-CANCER ; TOF MS ; PROTEINS ; IDENTIFICATION ; PEPTIDES ; OPPORTUNITIES
英文摘要

In this study, we describe characterization of the human plasma proteome based on analysis with multifunctional chitosan-GMA-IDA-Cu(II) nanospheres. Chitosan-GMA-IDA-Cu(II) nanospheres with diameters of 20 to 100 nm have unique properties due to multifunctional chemical moieties, high surface area, high capacity, good dispersibility in buffer solution as well as good biocompatibility and chemical stability which improves their specific interaction with peptides and proteins of the human plasma using different binding buffers. Combining these chitosan-GMA-IDA-Cu(II) nanospheres with MS spectrometry results in a novel strategy which should make it possible to characterize the plasma proteome in a single test. Peptides and proteins adsorbed on the nanosphere can be directly detected by MALDI-TOF-MS. The eluted lower molecular weight peptides and proteins are identified by nano-LC-ESI-MS/MS. A total of 842 unique LMW peptides and 1,682 human unredundant proteins IDs were identified in two different binding buffers, which included relatively low-level proteins (e.g., pg/mL of IL3 Interleukin-3) co-distributed with high-abundance proteins (e.g., 35-55 mg/mL level serum albumin). As such, this nanosphere technique selectively enabled the identification of proteins over a dynamic range of greater than 8 orders of magnitude. Considering this capacity for selective enrichment of peptides and proteins in human plasma, and the large number of LMW peptides and proteins which can be identified, this method promises to accelerate discovery of biomarkers for clinical application.

语种英语
WOS记录号WOS:000289297000018
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55166
专题北京大学医药卫生分析中心_研发办公室
北京大学医学信息学中心
北京大学医学部管理机构_医学部
北京大学基础医学院
作者单位1.Peking Univ, Prote Lab, Med & Healthy Analyt Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Leopold Franzens Univ, Inst Analyt Chem & Radiochem, A-6020 Innsbruck, Austria
推荐引用方式
GB/T 7714
Zou, Xiajuan,Zhong, Lijun,Liu, Dan,et al. Novel multifunctional chitosan-GMA-IDA-Cu(II) nanospheres for high dynamic range characterization of the human plasma proteome[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,400(3):747-756.
APA Zou, Xiajuan.,Zhong, Lijun.,Liu, Dan.,Yang, Bin.,Lou, Yaxin.,...&Yin, Yuxin.(2011).Novel multifunctional chitosan-GMA-IDA-Cu(II) nanospheres for high dynamic range characterization of the human plasma proteome.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,400(3),747-756.
MLA Zou, Xiajuan,et al."Novel multifunctional chitosan-GMA-IDA-Cu(II) nanospheres for high dynamic range characterization of the human plasma proteome".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 400.3(2011):747-756.
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