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学科主题: 基础医学
题名:
Novel multifunctional chitosan-GMA-IDA-Cu(II) nanospheres for high dynamic range characterization of the human plasma proteome
作者: Zou, Xiajuan1; Zhong, Lijun1; Liu, Dan1; Yang, Bin1; Lou, Yaxin1; Peng, Jiarou1; Rainer, Matthias3; Feuerstein, Isabel3; Muhammad, Najam-ul-Haq3; Huck, Christian W.3; Bonn, Guenther K.3; Yin, Yuxin1,2
关键词: Plasma ; Nanosphere ; Biomarkers ; Chitosan-GMA-IDA-Cu(II) ; MALDI-TOF-MS
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2011-05-01
DOI: 10.1007/s00216-011-4812-y
卷: 400, 期:3, 页:747-756
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: HUMAN SERUM PROTEOME ; MASS-SPECTROMETRY ; 2-DIMENSIONAL ELECTROPHORESIS ; CANCER BIOMARKERS ; OVARIAN-CANCER ; TOF MS ; PROTEINS ; IDENTIFICATION ; PEPTIDES ; OPPORTUNITIES
英文摘要:

In this study, we describe characterization of the human plasma proteome based on analysis with multifunctional chitosan-GMA-IDA-Cu(II) nanospheres. Chitosan-GMA-IDA-Cu(II) nanospheres with diameters of 20 to 100 nm have unique properties due to multifunctional chemical moieties, high surface area, high capacity, good dispersibility in buffer solution as well as good biocompatibility and chemical stability which improves their specific interaction with peptides and proteins of the human plasma using different binding buffers. Combining these chitosan-GMA-IDA-Cu(II) nanospheres with MS spectrometry results in a novel strategy which should make it possible to characterize the plasma proteome in a single test. Peptides and proteins adsorbed on the nanosphere can be directly detected by MALDI-TOF-MS. The eluted lower molecular weight peptides and proteins are identified by nano-LC-ESI-MS/MS. A total of 842 unique LMW peptides and 1,682 human unredundant proteins IDs were identified in two different binding buffers, which included relatively low-level proteins (e.g., pg/mL of IL3 Interleukin-3) co-distributed with high-abundance proteins (e.g., 35-55 mg/mL level serum albumin). As such, this nanosphere technique selectively enabled the identification of proteins over a dynamic range of greater than 8 orders of magnitude. Considering this capacity for selective enrichment of peptides and proteins in human plasma, and the large number of LMW peptides and proteins which can be identified, this method promises to accelerate discovery of biomarkers for clinical application.

语种: 英语
项目资助者: "985 project" of China ; BMWF Ministry for Science and Research, Vienna, Austria ; Eurasia Pacific Uninet
WOS记录号: WOS:000289297000018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55166
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Prote Lab, Med & Healthy Analyt Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Leopold Franzens Univ, Inst Analyt Chem & Radiochem, A-6020 Innsbruck, Austria

Recommended Citation:
Zou, Xiajuan,Zhong, Lijun,Liu, Dan,et al. Novel multifunctional chitosan-GMA-IDA-Cu(II) nanospheres for high dynamic range characterization of the human plasma proteome[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,400(3):747-756.
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