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Rapid chemical analysis of bear bile: 5 minute separation and quantitation of bile acids using UHPLC-qTOF-MS
Qiao, Xue1; Song, Wei1; Lin, Xiong-hao1; Wang, Qi1; Bo, Tao3; Guo, De-an1; Liu, Junxiu2; Ye, Min1
刊名ANALYTICAL METHODS
2014
DOI10.1039/c3ay41605d
6期:2页:596-601
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
研究领域[WOS]Chemistry ; Food Science & Technology ; Spectroscopy
关键词[WOS]URSODEOXYCHOLIC ACID ; METABOLISM ; PROTEINS
英文摘要

Bear bile is a precious traditional Chinese medicine containing abundant bile acids. The content of these bile acids varies significantly. In this study, a rapid and reliable method was established to simultaneously monitor major and minor bile acids, using ultra-high performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry. The samples were separated on a 1.8 mu m Zorbax Eclipse-Plus C-18 column with acetonitrile and water (containing 4 mM of ammonium acetate) as the mobile phase. A 5 min analysis allowed the characterization of 21 steroids and the quantitation of two major constituents, taurochenodeoxycholic acid (TCDCA) and tauroursodeoxycholic acid (TUDCA). The contents of TUDCA and TCDCA in 8 commercial batches of bear bile were 39.04 +/- 7.83% and 36.10 +/- 9.42% (w/w; mean +/- standard deviation), respectively, and the ratio of TUDCA/TCDCA ranged from 0.82 to 1.76. The simultaneous qualitative and quantitative analyses of bear bile powder was achieved, and minor steroid constituents were identified.

语种英语
WOS记录号WOS:000329072200037
项目编号81222054 ; NCET-11-0019
资助机构National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from the Chinese Ministry of Education
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55174
专题北京大学药学院
作者单位1.Agilent Technol, Beijing 100102, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Dept Otorhinolaryngol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Qiao, Xue,Song, Wei,Lin, Xiong-hao,et al. Rapid chemical analysis of bear bile: 5 minute separation and quantitation of bile acids using UHPLC-qTOF-MS[J]. ANALYTICAL METHODS,2014,6(2):596-601.
APA Qiao, Xue.,Song, Wei.,Lin, Xiong-hao.,Wang, Qi.,Bo, Tao.,...&Ye, Min.(2014).Rapid chemical analysis of bear bile: 5 minute separation and quantitation of bile acids using UHPLC-qTOF-MS.ANALYTICAL METHODS,6(2),596-601.
MLA Qiao, Xue,et al."Rapid chemical analysis of bear bile: 5 minute separation and quantitation of bile acids using UHPLC-qTOF-MS".ANALYTICAL METHODS 6.2(2014):596-601.
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