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Cooperative interaction between the basic helix-loop-helix transcription factor dHAND and myocyte enhancer factor 2C regulates myocardial gene expression
Zang, MX; Li, Y; Wang, H; Wang, JB; Jia, HT
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
2004-12-24
DOI10.1074/jbc.M408502200
279期:52页:54258-54263
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]ACTIVATION ; MEF2 ; PROTEIN ; PROMOTER ; BINDING ; ROLES ; HAND2 ; TBX5 ; MYOD
英文摘要

Cardiac-restricted transcription factors dHAND and myocyte enhancer factor 2C are expressed in the developing heart and activate several cardiac promoters. However, their regulatory mechanisms are still to be understood. To elucidate their exact regulatory functions, we have developed an RNA interference strategy to specifically inhibit dHAND and myocyte enhancer factor 2C protein production in H9c2 cells, which are derived from rat embryonic heart. Expression of endogenous cardiac genes atrial natriuretic peptide and alpha-myosin heavy chain was down-regulated in H9c2 cells lacking both dHAND and myocyte enhancer factor 2C, indicating that these factors are required for the maintenance of the cardiac genetic program. Consistent with these, expression of atrial natriuretic peptide and alpha-myosin heavy chain was up-regulated in H9c2 cells, which overexpressed dHAND and myocyte enhancer factor 2C. In addition, dHAND and myocyte enhancer factor 2C interact to synergistically activate atrial natriuretic peptide and alpha-myosin heavy chain transcription. Furthermore, chromatin immunoprecipitation analysis in H9c2 cells treated with phenylephrine showed that dHAND and myocyte enhancer factor 2C protein complex bind to the A/T sequence on atrial natriuretic peptide promoter. Taken together, these results not only suggest that the complex cis-trans interaction of dHAND, myocyte enhancer factor 2C, and the target gene may fine-tune gene expression in cardiac myocytes but also provide a molecular paradigm to elucidate the mechanisms of action of dHAND and myocyte enhancer factor 2C in the developing heart.

语种英语
WOS记录号WOS:000225793600046
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55178
专题北京大学公共卫生学院
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Publ Hlth, Dept Nutr & Food Hyg,Lab Dev Mol Biol, Beijing 100083, Peoples R China
2.Clin Res Inst Montreal, Lab Cardiac Growth & Differentiat, Montreal, PQ H2W 1R7, Canada
3.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
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GB/T 7714
Zang, MX,Li, Y,Wang, H,et al. Cooperative interaction between the basic helix-loop-helix transcription factor dHAND and myocyte enhancer factor 2C regulates myocardial gene expression[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2004,279(52):54258-54263.
APA Zang, MX,Li, Y,Wang, H,Wang, JB,&Jia, HT.(2004).Cooperative interaction between the basic helix-loop-helix transcription factor dHAND and myocyte enhancer factor 2C regulates myocardial gene expression.JOURNAL OF BIOLOGICAL CHEMISTRY,279(52),54258-54263.
MLA Zang, MX,et al."Cooperative interaction between the basic helix-loop-helix transcription factor dHAND and myocyte enhancer factor 2C regulates myocardial gene expression".JOURNAL OF BIOLOGICAL CHEMISTRY 279.52(2004):54258-54263.
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