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学科主题: 基础医学
题名:
Autophagic effect of programmed cell death 5 (PDCD5) after focal cerebral ischemic reperfusion injury in rats
作者: Jiang, Zhao1; Chen, Chun-Hua1; Chen, Ying-Yu2; Han, Jing-Yan3; Riley, John4; Zhou, Chang-Man1
关键词: MCAO ; PDCD5 ; Autophagy ; MRI ; siRNA
刊名: NEUROSCIENCE LETTERS
发表日期: 2014-04-30
DOI: 10.1016/j.neulet.2014.02.066
卷: 566, 期:0, 页:298-303
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: DOUBLE-EDGED-SWORD ; ARTERY OCCLUSION ; HYPERBARIC-OXYGEN ; INDUCED APOPTOSIS ; MODEL ; INHIBITION ; PROTEINS ; BECLIN-1
英文摘要:

Former studies indicated that programmed cell death 5 (PDCD5) protein could accelerate the process of apoptosis in response to some stimuli in various kinds of cells via the intrinsic or extrinsic pathway. In this study, we aimed to demonstrate for the first time that protein level of PDCD5 are related to autophagic activity after focal ischemic brain injury in rats. One hundred and twenty-five Sprague-Dawley rats (male) were randomly divided into the following groups: Sham operated, Middle Cerebral Artery Occlusion/Reperfusion (MCAO), MCAO + Control siRNA and MCAO + PDCD5 siRNA. Outcome measurements include neurobehavioral outcomes, brain infarct volume, brain water content, BBB disruption, MRI and double fluorescence labeling. Western blot and histopathophysiological techniques were used to measure the expression of PDCD5 and some pro-autophagic proteins such as Beclin 1 and the LC3-II/LC3-I ratio. The study found that decreased PDCD5 expression via intracerebroventricular injection of PDCD5 siRNA significantly improved the neurobehavioral outcome, reduced the infarct ratio, cerebral edema and BBB disruption. These results were associated with decreased expression of Beclin 1 and the LC3-II/LC3-I ratio in the penumbra area. Rapamycin, an inducer of autophagy, partially weakened the effect of PDCD5 siRNA. In conclusion, this study suggested that PDCD5 was a key regulator of autophagy that might play an important role following MCAO injury. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 81000523 ; 31271280
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000335613800058
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55185
Appears in Collections:基础医学院_北京大学人类疾病基因研究中心_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Anatomyand Embryol, Beijing 100191, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100871, Peoples R China
3.State Adm Tradit Chinese Med, Key Lab Stasis & Phlegm, Beijing, Peoples R China
4.Hosp Univ Penn, Dept Anesthesiol & Crit Care, Philadelphia, PA 19104 USA

Recommended Citation:
Jiang, Zhao,Chen, Chun-Hua,Chen, Ying-Yu,et al. Autophagic effect of programmed cell death 5 (PDCD5) after focal cerebral ischemic reperfusion injury in rats[J]. NEUROSCIENCE LETTERS,2014,566(0):298-303.
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