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Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats
Liu, Xiaomin1; Hu, Rui2; Lian, Hongwei1,3; Liu, Yang4; Liu, Jing1; Liu, Jianwei1; Lin, Guimiao5; Liu, Liwei6; Duan, Xiaojian1; Yong, Ken-Tye2; Ye, Ling1
关键词Diabetic Nephropathy Tlr4 Immunofluorescence Immunohistochemistry
刊名INTERNATIONAL JOURNAL OF NANOMEDICINE
2015
DOI10.2147/IJN.S81395
10页:3651-3662
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
资助者National Natural Science Foundation of China ; Singapore Ministry of Education ; A*STAR Science and Engineering Research Council ; Nanyang Institute of Technology in Health and Medicine ; National Natural Science Foundation of China ; Singapore Ministry of Education ; A*STAR Science and Engineering Research Council ; Nanyang Institute of Technology in Health and Medicine
研究领域[WOS]Science & Technology - Other Topics ; Pharmacology & Pharmacy
关键词[WOS]OXIDATIVE STRESS ; SORBITOL PATHWAY ; CELLULAR TARGETS ; LIVE CELLS ; EXPRESSION ; COMPLICATIONS ; NEPHROPATHY ; RECOGNITION ; METABOLISM ; CANCER
英文摘要

Diabetes is one of the major chronic diseases diagnosed worldwide with a common complication of diabetic nephropathy (DN). There are multiple possible mechanisms associated with DN. Aldose reductase (AR) and Toll-like receptor 4 (TLR4) may be involved in the occurrence and development of DN. Here, we describe the distribution of AR and TLR4 in cells and renal tissues of diabetic rats through a quantum dot (QD)-based immunofluorescence technique and conventional immunohistochemistry. As a new type of nanosized fluorophore, QDs have been recognized in imaging applications and have broad prospects in biomedical research. The results of the reported study demonstrate that both the AR and the TLR4 proteins were upregulated in the renal tissues of diabetic rats. Further, to explore the relationship between AR and TLR4 in the pathogenesis of DN, a dual-color immunofluorescent labeling technique based on QDs was applied, where the expressions of AR and TLR4 in the renal tissues of diabetic rats were simultaneously observed -for the first time, as far as we are aware. The optimized QD-based immunofluorescence technique has not only shown a satisfying sensitivity and specificity for the detection of biomarkers in cells and tissues, but also is a valuable supplement of immunohistochemistry. The QD-based multiplexed imaging technology provides a new insight into the mechanistic study of the correlation among biological factors as well as having potential applications in the diagnosis and treatment of diseases.

语种英语
所属项目编号51371197 ; 21071150 ; 61107017 ; MOE2010-T2-2-010 (4020020.040 ARC2/11) ; M4070176.040.703142 ; M4061202.040
资助者National Natural Science Foundation of China ; Singapore Ministry of Education ; A*STAR Science and Engineering Research Council ; Nanyang Institute of Technology in Health and Medicine ; National Natural Science Foundation of China ; Singapore Ministry of Education ; A*STAR Science and Engineering Research Council ; Nanyang Institute of Technology in Health and Medicine
WOS记录号WOS:000354785500001
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55186
专题北京大学第三临床医学院_急诊科
作者单位1.Chinese Peoples Liberat Army Gen Hosp, Inst Gerontol & Geriatr, Beijing Key Lab Aging & Geriatr, Beijing 100853, Peoples R China
2.Nanyang Technol Univ, Sch Elect & Elect Engn, Singapore 639798, Singapore
3.Peking Univ, Hosp 3, Dept Emergency Med, Beijing 100871, Peoples R China
4.Chinese Peoples Liberat Army Gen Hosp, Dept Geriatr Nephrol, Beijing 100853, Peoples R China
5.Shenzhen Univ, Sch Med Sci, Key Lab Biomed Engn, Shenzhen, Peoples R China
6.Changchun Univ Sci & Technol, Sch Sci, Changchun, Peoples R China
推荐引用方式
GB/T 7714
Liu, Xiaomin,Hu, Rui,Lian, Hongwei,et al. Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2015,10:3651-3662.
APA Liu, Xiaomin.,Hu, Rui.,Lian, Hongwei.,Liu, Yang.,Liu, Jing.,...&Ye, Ling.(2015).Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats.INTERNATIONAL JOURNAL OF NANOMEDICINE,10,3651-3662.
MLA Liu, Xiaomin,et al."Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats".INTERNATIONAL JOURNAL OF NANOMEDICINE 10(2015):3651-3662.
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