IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
Hydrogen Peroxide Enhances Vasodilatation by Increasing Dimerization of cGMP-Dependent Protein Kinase Type I alpha
Dou, Dou1,2; Zheng, Xiaoxu1; Liu, Juan1; Xu, Xiaojian1; Ye, Liping1; Gao, Yuansheng1,2
关键词Coronary Circulation Reactive Oxygen Species Vasodilation
刊名CIRCULATION JOURNAL
2012-07-01
DOI10.1253/circj.CJ-11-1368
76期:7页:1792-1798
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems
资助者National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education for New Teachers ; Peking University Health Science Center Foundation for New Teachers ; National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education for New Teachers ; Peking University Health Science Center Foundation for New Teachers
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]PORCINE CORONARY-ARTERIES ; SMOOTH-MUSCLE ; HYPERPOLARIZING FACTOR ; OXIDATIVE STRESS ; NITRIC-OXIDE ; ENDOTHELIUM ; ACTIVATION ; CATALASE ; DYSFUNCTION ; RELAXATION
英文摘要

Background: cGMP-dependent protein kinase type I (PKG I) plays a key role in vasodilatation caused by cGMP-elevating agents. It is a homodimer in mammalian cells, existing as 2 isoforms, I alpha and I beta. The aim of the present study was both to determine whether PKG I dimerization and activity are modulated by hydrogen peroxide (H2O2) and its influence on vasodilatation.

Methods and Results: The dimers and monomers of total PKG I and PKG I beta were analyzed by Western blotting. PKG I activity was assayed by measuring the incorporation of P-32 into BPDEtide. Changes in vessels tension were determined by organ chamber technique. In isolated porcine coronary arteries, H2O2 increased the dimers of total PKG I in a concentration-dependent manner, but had no effect on dimerization of PKG 1 beta. The dimerization of PKG I caused by H2O2 was prevented by catalase but not by deferoxamine and tiron. H2O2 promoted the translocation of PKG I from cytoplasm to membrane. H2O2 enhanced the activity of PKG I and relaxations of porcine coronary arteries to the nitric oxide donor and 8-Br-cGMP. Inhibition of catalase under in vivo conditions significantly decreased rat mean arterial pressure, which was associated with increased dimerization of PKG I.

Conclusions: The present study suggests that H2O2 may enhance the activity of PKG I alpha- and PKG I-dependent vasodilatation via increased dimerization of the enzyme. (Circ J 2012; 76: 1792-1798)

语种英语
所属项目编号30870938 ; 81001433 ; 20100001120037 ; BMU20090510
资助者National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education for New Teachers ; Peking University Health Science Center Foundation for New Teachers ; National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education for New Teachers ; Peking University Health Science Center Foundation for New Teachers
WOS记录号WOS:000305869300038
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55195
Collection北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
2.Peking Univ, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Dou, Dou,Zheng, Xiaoxu,Liu, Juan,et al. Hydrogen Peroxide Enhances Vasodilatation by Increasing Dimerization of cGMP-Dependent Protein Kinase Type I alpha[J]. CIRCULATION JOURNAL,2012,76(7):1792-1798.
APA Dou, Dou,Zheng, Xiaoxu,Liu, Juan,Xu, Xiaojian,Ye, Liping,&Gao, Yuansheng.(2012).Hydrogen Peroxide Enhances Vasodilatation by Increasing Dimerization of cGMP-Dependent Protein Kinase Type I alpha.CIRCULATION JOURNAL,76(7),1792-1798.
MLA Dou, Dou,et al."Hydrogen Peroxide Enhances Vasodilatation by Increasing Dimerization of cGMP-Dependent Protein Kinase Type I alpha".CIRCULATION JOURNAL 76.7(2012):1792-1798.
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