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学科主题: 基础医学
题名:
Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells
作者: Guo, S; Sato, T; Shirane, K; Furukawa, K
关键词: acceptor specificity ; in vivo beta-1,4-galactosylation ; K-m value ; N-glycan
刊名: GLYCOBIOLOGY
发表日期: 2001-10-01
卷: 11, 期:10, 页:813-820
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: BETA-4-GALACTOSYLTRANSFERASE GENE FAMILY ; BOVINE-MILK GALACTOSYLTRANSFERASE ; UDP-GALACTOSE ; INSECT CELLS ; ACETYLLACTOSAMINE SYNTHESIS ; MEMBRANE-GLYCOPROTEINS ; POLYSIALIC ACID ; CDNA CLONING ; GLYCANS ; PURIFICATION
英文摘要:

Several studies showed that Sf-9 cells can synthesize the galactosylated N-linked oligosaccharides if beta -1,4-galactosyltransferase (beta -1,4-GalT) is supplied. The full-length human beta -1,4-GalT I, II, III, IV, V, and VI cDNAs were independently transfected into Sf-9 cells, and the galactosylation of endogenous membrane glycoproteins was examined by lectin blot analysis using Ricinus communis agglutinin-I (RCA-I), which preferentially interacts with oligosaccharides terminated with Gal beta1 --> 4GlcNAc group. Several RCA-I-reactive bands appeared in all of the gene-transfected cells, and disappeared on treatment of blots with beta -1,4-galactosidase or N-glycanase prior to incubation with lectin. Introduction of the antisense beta -1,4-GalT II and V cDNAs separately into human colorectal adenocarcinoma SW480 cells, in which beta -1,4-GalT I, II, and V genes were expressed, resulted in the reduction of RCA-I binding toward N-linked oligosaccharides of the membrane glycoproteins. Differences were found in their K-m. values toward UDP-Gal and GlcNAc beta -S-pNP and in their acceptor specificities toward oligosaccharides with the GlcNAc beta1 -->4(GlcNAc beta1 -->2)Man branch and with the GlcNAc beta1 -->6(GlcNAc beta1 -->2)Man branch. These results indicate that beta -1,4-GalTs II, III, IV, V, and VI are involved in the N-linked oligosaccharide biosynthesis cooperatively but not in a redundanat manner with beta -1,4-GalT I within cells.

语种: 英语
WOS记录号: WOS:000171457800004
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55203
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Beijing Med Univ, Dept Pathol, Beijing 100083, Peoples R China
2.Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, Itabashi Ku, Tokyo 1730015, Japan
3.Nisshin Flour Milling Co Ltd, Saitama 3568511, Japan

Recommended Citation:
Guo, S,Sato, T,Shirane, K,et al. Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells[J]. GLYCOBIOLOGY,2001,11(10):813-820.
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