北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 病理学系  > 期刊论文
学科主题: 基础医学
题名:
Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells
作者: Guo, S; Sato, T; Shirane, K; Furukawa, K
关键词: acceptor specificity ; in vivo beta-1,4-galactosylation ; K-m value ; N-glycan
刊名: GLYCOBIOLOGY
发表日期: 2001-10-01
卷: 11, 期:10, 页:813-820
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: BETA-4-GALACTOSYLTRANSFERASE GENE FAMILY ; BOVINE-MILK GALACTOSYLTRANSFERASE ; UDP-GALACTOSE ; INSECT CELLS ; ACETYLLACTOSAMINE SYNTHESIS ; MEMBRANE-GLYCOPROTEINS ; POLYSIALIC ACID ; CDNA CLONING ; GLYCANS ; PURIFICATION
英文摘要:

Several studies showed that Sf-9 cells can synthesize the galactosylated N-linked oligosaccharides if beta -1,4-galactosyltransferase (beta -1,4-GalT) is supplied. The full-length human beta -1,4-GalT I, II, III, IV, V, and VI cDNAs were independently transfected into Sf-9 cells, and the galactosylation of endogenous membrane glycoproteins was examined by lectin blot analysis using Ricinus communis agglutinin-I (RCA-I), which preferentially interacts with oligosaccharides terminated with Gal beta1 --> 4GlcNAc group. Several RCA-I-reactive bands appeared in all of the gene-transfected cells, and disappeared on treatment of blots with beta -1,4-galactosidase or N-glycanase prior to incubation with lectin. Introduction of the antisense beta -1,4-GalT II and V cDNAs separately into human colorectal adenocarcinoma SW480 cells, in which beta -1,4-GalT I, II, and V genes were expressed, resulted in the reduction of RCA-I binding toward N-linked oligosaccharides of the membrane glycoproteins. Differences were found in their K-m. values toward UDP-Gal and GlcNAc beta -S-pNP and in their acceptor specificities toward oligosaccharides with the GlcNAc beta1 -->4(GlcNAc beta1 -->2)Man branch and with the GlcNAc beta1 -->6(GlcNAc beta1 -->2)Man branch. These results indicate that beta -1,4-GalTs II, III, IV, V, and VI are involved in the N-linked oligosaccharide biosynthesis cooperatively but not in a redundanat manner with beta -1,4-GalT I within cells.

语种: 英语
WOS记录号: WOS:000171457800004
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55203
Appears in Collections:基础医学院_病理学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells.pdf(392KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Beijing Med Univ, Dept Pathol, Beijing 100083, Peoples R China
2.Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, Itabashi Ku, Tokyo 1730015, Japan
3.Nisshin Flour Milling Co Ltd, Saitama 3568511, Japan

Recommended Citation:
Guo, S,Sato, T,Shirane, K,et al. Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells[J]. GLYCOBIOLOGY,2001,11(10):813-820.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Guo, S]'s Articles
[Sato, T]'s Articles
[Shirane, K]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Guo, S]‘s Articles
[Sato, T]‘s Articles
[Shirane, K]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace