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学科主题: 临床医学
题名:
Combined Porin Loss and Extended Spectrum beta-Lactamase Production is Associated with an Increasing Imipenem Minimal Inhibitory Concentration in Clinical Klebsiella pneumoniae Strains
作者: Yang, Duo1,2; Guo, Yu1,2; Zhang, Zheng1,2
刊名: CURRENT MICROBIOLOGY
发表日期: 2009-04-01
DOI: 10.1007/s00284-009-9364-4
卷: 58, 期:4, 页:366-370
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: ESCHERICHIA-COLI ; CARBAPENEM RESISTANCE ; ANTIBIOTIC-RESISTANCE ; SUSCEPTIBILITY ; COMBINATION ; PREVALENCE ; EXPRESSION ; HOSPITALS ; AGENTS ; SHV-2
英文摘要:

For this study, 150 clinical isolates of Klebsiella pneumoniae were collected from one hospital in Beijing, China, and assayed for minimal inhibitory concentration (MIC) of imipenem. To elucidate the mechanisms responsible for imipenem MIC variation among extended-spectrum beta-lactamase (ESBL)-positive and -negative K. pneumoniae strains, a variety of beta-lactamase genes (bla (TEM), bla (CTX-M), bla (SHV), and bla (OXA)) were screened by polymerase chain reaction (PCR). The outer membrane profile and expression of related genes (ompK35 and ompK36) then were analyzed and evaluated, respectively. None of the tested isolates were clinically resistant to imipenem, but the range of MICs among ESBL-positive and -negative strains was significantly different. Deficiency in the expression of outer membrane proteins (OmpK35,36) was observed in some of both ESBL-positive(17.6%) and -negative strains (10.9%), but only the ESBL-positive strains depressed by the expression of ompK35/36 had an increased MIC of imipenem (a parts per thousand yen0.5 mg/l). These results confirmed that the combination of SHV-1, CTX-M-3, CTX-M-14, TEM-1, or OXA-11 production and reduced expression of ompK35/36 may not result in clinical resistance to imipenem but does correlate with increasing imipenem MIC.

语种: 英语
项目资助者: Peking University
WOS记录号: WOS:000263869200015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55226
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ Peoples Hosp, Dept Clin Lab, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Clin Lab, Beijing 100044, Peoples R China

Recommended Citation:
Yang, Duo,Guo, Yu,Zhang, Zheng. Combined Porin Loss and Extended Spectrum beta-Lactamase Production is Associated with an Increasing Imipenem Minimal Inhibitory Concentration in Clinical Klebsiella pneumoniae Strains[J]. CURRENT MICROBIOLOGY,2009,58(4):366-370.
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