IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
Expression, purification and serological analysis of hepatocellular carcinoma associated antigen HCA587 in insect cells
Li, B; Wu, HY; Qian, XP; Li, Y; Chen, WF
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2003-04-01
9期:4页:678-682
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]CYTOLYTIC T-LYMPHOCYTES ; HUMAN TUMOR-ANTIGENS ; CANCER/TESTIS ANTIGENS ; CANCER-IMMUNOTHERAPY ; METASTATIC MELANOMA ; IMMUNE-RESPONSES ; IDENTIFICATION ; FAMILY ; GENE ; PROTEIN
英文摘要

AIM: In order to assess hepatocellular carcinoma associated antigen HCA587 as a potential target for immunotherapy, the Bac-to-Bac expression system was used to express recombinant protein HCA587 in insect cells.

METHODS: The cDNA encoding HCA587 gene was cloned into donor vector pFasBacHtb and recombinant pFasBac Htb-587 was transformed into competent cells DH10Bac. Recombinant Bacmid-587 was transfected into Sf9 insect cells using CELLFECTIN, Recombinant HCA587 protein was produced in Sf9 insect cells after infection with recombinant baculovirus, and was purified using Ni-NTA resin. Sera from HCC patients were also screened using recombinant protein HCA587.

RESULTS: The molecular weight of the recombinant protein HCA587 expressed in insect cells was approximately 43kd. Western blot results proved the recombinant protein HCA587 had the similar antigenicity with its native counterpart. Serological analysis told that the rate of seroreactivity to HCA587 was not high in HCC patients.

CONCLUSION: The recombinant protein HCA587 was successfully expressed and purified using Bac-to-Bac expression system. It paved the way for generation of specific antibody and investigation of immunohistochemical analysis and immune responses of HCC in the future.

语种英语
WOS记录号WOS:000182469000010
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55227
专题北京大学基础医学院_免疫学系
作者单位Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Li, B,Wu, HY,Qian, XP,et al. Expression, purification and serological analysis of hepatocellular carcinoma associated antigen HCA587 in insect cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2003,9(4):678-682.
APA Li, B,Wu, HY,Qian, XP,Li, Y,&Chen, WF.(2003).Expression, purification and serological analysis of hepatocellular carcinoma associated antigen HCA587 in insect cells.WORLD JOURNAL OF GASTROENTEROLOGY,9(4),678-682.
MLA Li, B,et al."Expression, purification and serological analysis of hepatocellular carcinoma associated antigen HCA587 in insect cells".WORLD JOURNAL OF GASTROENTEROLOGY 9.4(2003):678-682.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
WJG-9-678.pdf(143KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, B]的文章
[Wu, HY]的文章
[Qian, XP]的文章
百度学术
百度学术中相似的文章
[Li, B]的文章
[Wu, HY]的文章
[Qian, XP]的文章
必应学术
必应学术中相似的文章
[Li, B]的文章
[Wu, HY]的文章
[Qian, XP]的文章
相关权益政策
暂无数据
收藏/分享
文件名: WJG-9-678.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。