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学科主题: 基础医学
题名:
Expression, purification and serological analysis of hepatocellular carcinoma associated antigen HCA587 in insect cells
作者: Li, B; Wu, HY; Qian, XP; Li, Y; Chen, WF
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2003-04-01
卷: 9, 期:4, 页:678-682
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: CYTOLYTIC T-LYMPHOCYTES ; HUMAN TUMOR-ANTIGENS ; CANCER/TESTIS ANTIGENS ; CANCER-IMMUNOTHERAPY ; METASTATIC MELANOMA ; IMMUNE-RESPONSES ; IDENTIFICATION ; FAMILY ; GENE ; PROTEIN
英文摘要:

AIM: In order to assess hepatocellular carcinoma associated antigen HCA587 as a potential target for immunotherapy, the Bac-to-Bac expression system was used to express recombinant protein HCA587 in insect cells.

METHODS: The cDNA encoding HCA587 gene was cloned into donor vector pFasBacHtb and recombinant pFasBac Htb-587 was transformed into competent cells DH10Bac. Recombinant Bacmid-587 was transfected into Sf9 insect cells using CELLFECTIN, Recombinant HCA587 protein was produced in Sf9 insect cells after infection with recombinant baculovirus, and was purified using Ni-NTA resin. Sera from HCC patients were also screened using recombinant protein HCA587.

RESULTS: The molecular weight of the recombinant protein HCA587 expressed in insect cells was approximately 43kd. Western blot results proved the recombinant protein HCA587 had the similar antigenicity with its native counterpart. Serological analysis told that the rate of seroreactivity to HCA587 was not high in HCC patients.

CONCLUSION: The recombinant protein HCA587 was successfully expressed and purified using Bac-to-Bac expression system. It paved the way for generation of specific antibody and investigation of immunohistochemical analysis and immune responses of HCC in the future.

语种: 英语
WOS记录号: WOS:000182469000010
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55227
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Li, B,Wu, HY,Qian, XP,et al. Expression, purification and serological analysis of hepatocellular carcinoma associated antigen HCA587 in insect cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2003,9(4):678-682.
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