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Interferon regulatory factor-1 mediates PPAR gamma-induced apoptosis in vascular smooth muscle cells
Lin, YM; Zhu, XJ; Mclntee, FL; Xiao, HL; Zhang, JF; Fu, MG; Chen, YQE
关键词Peroxisome Proliferator-activated Receptor Gamma Interferon Regulatory Factor-1 Vascular Smooth Muscle Cells Gene Expression Apoptosis Atherosclerosis
刊名ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
2004-02-01
DOI10.1161/01.ATV.0000109170.43400.2f
24期:2页:257-263
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Hematology ; Peripheral Vascular Disease
研究领域[WOS]Hematology ; Cardiovascular System & Cardiology
关键词[WOS]ACTIVATED-RECEPTOR-GAMMA ; DEPENDENT KINASE INHIBITORS ; TRANSCRIPTION FACTOR IRF-1 ; LOW-DENSITY-LIPOPROTEIN ; GENE-EXPRESSION ; 15-DEOXY-DELTA(12,14)-PROSTAGLANDIN J(2) ; INDEPENDENT PATHWAY ; INTIMAL HYPERPLASIA ; PROTEIN-KINASE ; DEFICIENT MICE
英文摘要

Objective-Peroxisome proliferator-activated receptor gamma (PPARgamma) possesses general beneficial effects on the cardiovascular system, such as inhibition of vascular lesion formation and atherosclerosis. However, molecular mechanisms for these effects are yet to be fully defined. The aim of this study is to elucidate whether interferon regulatory factor-1 (IRF-1), a transcriptional factor with anti-proliferative and pro-apoptotic properties, mediates PPARgamma-induced apoptosis in vascular smooth muscle cells (VSMCs).

Methods and Results-Using Northern and Western blot analyses, we documented that PPARgamma ligands, including ciglitazone, troglitazone, and GW7845, significantly increased IRF-1 expression in VSMCs; however, the PPARalpha ligand (Wy14643) and PPARdelta ligand (GW0742) did not affect its expression. PPARgamma-induced IRF-1 expression was abrogated by pretreatment with the PPARgamma antagonist GW9662. In contrast, adenoviral expression of PPARgamma in VSMCs dramatically increased IRF-1 level. Furthermore, PPARgamma activation increased IRF-1 promoter activity but did not affect IRF-1 mRNA stability. Finally, reducing IRF-1 expression by antisense technology attenuated PPARgamma-induced VSMC apoptosis through decreasing cyclin-dependent kinase inhibitor p21(cip1) and caspase-3 activity.

Conclusion-Our data demonstrate that IRF-1 is a novel PPARgamma target gene and mediates PPARgamma-induced VSMC apoptosis.

语种英语
WOS记录号WOS:000188777400007
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55228
专题北京大学基础医学院_心血管所
作者单位1.Morehouse Sch Med, Cardiovasc Res Inst, Atlanta, GA 30310 USA
2.Peking Univ, Hlth Sci Ctr, Cardiovasc Res Inst, Beijing 100083, Peoples R China
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Lin, YM,Zhu, XJ,Mclntee, FL,et al. Interferon regulatory factor-1 mediates PPAR gamma-induced apoptosis in vascular smooth muscle cells[J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY,2004,24(2):257-263.
APA Lin, YM.,Zhu, XJ.,Mclntee, FL.,Xiao, HL.,Zhang, JF.,...&Chen, YQE.(2004).Interferon regulatory factor-1 mediates PPAR gamma-induced apoptosis in vascular smooth muscle cells.ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY,24(2),257-263.
MLA Lin, YM,et al."Interferon regulatory factor-1 mediates PPAR gamma-induced apoptosis in vascular smooth muscle cells".ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 24.2(2004):257-263.
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