北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 心血管所  > 期刊论文
学科主题: 基础医学
题名:
Interferon regulatory factor-1 mediates PPAR gamma-induced apoptosis in vascular smooth muscle cells
作者: Lin, YM; Zhu, XJ; Mclntee, FL; Xiao, HL; Zhang, JF; Fu, MG; Chen, YQE
关键词: peroxisome proliferator-activated receptor gamma interferon regulatory factor-1 ; vascular smooth muscle cells ; gene expression ; apoptosis ; atherosclerosis
刊名: ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
发表日期: 2004-02-01
DOI: 10.1161/01.ATV.0000109170.43400.2f
卷: 24, 期:2, 页:257-263
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology ; Peripheral Vascular Disease
研究领域[WOS]: Hematology ; Cardiovascular System & Cardiology
关键词[WOS]: ACTIVATED-RECEPTOR-GAMMA ; DEPENDENT KINASE INHIBITORS ; TRANSCRIPTION FACTOR IRF-1 ; LOW-DENSITY-LIPOPROTEIN ; GENE-EXPRESSION ; 15-DEOXY-DELTA(12,14)-PROSTAGLANDIN J(2) ; INDEPENDENT PATHWAY ; INTIMAL HYPERPLASIA ; PROTEIN-KINASE ; DEFICIENT MICE
英文摘要:

Objective-Peroxisome proliferator-activated receptor gamma (PPARgamma) possesses general beneficial effects on the cardiovascular system, such as inhibition of vascular lesion formation and atherosclerosis. However, molecular mechanisms for these effects are yet to be fully defined. The aim of this study is to elucidate whether interferon regulatory factor-1 (IRF-1), a transcriptional factor with anti-proliferative and pro-apoptotic properties, mediates PPARgamma-induced apoptosis in vascular smooth muscle cells (VSMCs).

Methods and Results-Using Northern and Western blot analyses, we documented that PPARgamma ligands, including ciglitazone, troglitazone, and GW7845, significantly increased IRF-1 expression in VSMCs; however, the PPARalpha ligand (Wy14643) and PPARdelta ligand (GW0742) did not affect its expression. PPARgamma-induced IRF-1 expression was abrogated by pretreatment with the PPARgamma antagonist GW9662. In contrast, adenoviral expression of PPARgamma in VSMCs dramatically increased IRF-1 level. Furthermore, PPARgamma activation increased IRF-1 promoter activity but did not affect IRF-1 mRNA stability. Finally, reducing IRF-1 expression by antisense technology attenuated PPARgamma-induced VSMC apoptosis through decreasing cyclin-dependent kinase inhibitor p21(cip1) and caspase-3 activity.

Conclusion-Our data demonstrate that IRF-1 is a novel PPARgamma target gene and mediates PPARgamma-induced VSMC apoptosis.

语种: 英语
WOS记录号: WOS:000188777400007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55228
Appears in Collections:基础医学院_心血管所_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
257.full.pdf(603KB)期刊论文作者接受稿限制开放 联系获取全文

作者单位: 1.Morehouse Sch Med, Cardiovasc Res Inst, Atlanta, GA 30310 USA
2.Peking Univ, Hlth Sci Ctr, Cardiovasc Res Inst, Beijing 100083, Peoples R China

Recommended Citation:
Lin, YM,Zhu, XJ,Mclntee, FL,et al. Interferon regulatory factor-1 mediates PPAR gamma-induced apoptosis in vascular smooth muscle cells[J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY,2004,24(2):257-263.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Lin, YM]'s Articles
[Zhu, XJ]'s Articles
[Mclntee, FL]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Lin, YM]‘s Articles
[Zhu, XJ]‘s Articles
[Mclntee, FL]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace