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学科主题: 药学
题名:
Determination of dehydrodiisoeugenol in rat tissues using HPLC method
作者: Li, Fei; Yang, Xiu-wei1
关键词: Myristica fragrans Houtt. ; dehydrodiisoeugenol ; tissue distribution ; HPLC
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2008-11-01
DOI: 10.1002/bmc.1046
卷: 22, 期:11, 页:1206-1212
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: MYRISTICA-FRAGRANS SEEDS
英文摘要:

Dehydrodiisoeugenol (DDIE) is a bioactive neolignan from the seeds of Myristica fragrans Houtt., which exhibits good anti-inflammatory activity. A rapid and simple high-performance liquid chromatographic (HPLC) method has been developed for the determination of DDIE in rat tissues after intravenous administration. The tissue samples were processed by liquid-liquid extraction. The analyses were successfully carried out on a Diamonsil (TM) ODS C(18) column (250 x 4.6 mm i.d., 5 mu m) equipped with a C(18) guard column (8 x 4.6 mm i.d, 5 mu m). The mobile phase was the system of methanol-water (4:1, v/v). The UV detection was set at 270 nm. The calibration curves were linear from 0.4 to 200.0 mu g/g with the correlation coefficients ) greater than 0.998. The intra- and inter-day precisions in quality control samples were less than 10% and the accuracies were in the range 85.4-110.3%. The average recoveries from all the tissues were between 84.4 and 106.0%. This assay method has been successfully used to study the tissues distribution of DDIE in rats after intravenous administration. The result suggests that DDIE is distributed to rat tissues rapidly with possibly greater initial concentrations in liver and brain than in other tissues. Copyright (c) 2008 John Wiley & Sons, Ltd.

语种: 英语
所属项目编号: 2002AA2Z343C ; 2004AA2Z3783 ; 2006BA106A01-02 ; Z0004105040311
项目资助者: National High Technology Research and Development Program of China ; Beijing Municipal Special-purpose Science Foundation of China
WOS记录号: WOS:000261262300005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55232
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Peking Univ, Dept Nat Med, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Li, Fei,Yang, Xiu-wei. Determination of dehydrodiisoeugenol in rat tissues using HPLC method[J]. BIOMEDICAL CHROMATOGRAPHY,2008,22(11):1206-1212.
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