学科主题基础医学
Hepatoblast-Like Progenitor Cells Derived From Embryonic Stem Cells Can Repopulate Livers of Mice
Li, Fuming1,2; Liu, Pingyu1,2; Liu, Changcheng1,3; Xiang, Dao3; Deng, Li4; Li, Wenlin3; Wangensteen, Kirk5,6; Song, Jianguo1; Ma, Yue7; Hui, Lijian1; Wei, Lixin8; Li, Lingsong9,10; Ding, Xiaoyan1; Hu, Yiping3; He, Zhiying3; Wang, Xin1,11,12
关键词Hepatic Differentiation Bipotency Cell Transplantation Liver Injury
刊名GASTROENTEROLOGY
2010-12-01
DOI10.1053/j.gastro.2010.08.042
139期:6页:2158-U495
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]MOUSE-LIVER ; IN-VIVO ; EFFICIENT DIFFERENTIATION ; DEFINITIVE ENDODERM ; HEPATIC ENDODERM ; HEPATOCYTES ; GENERATION ; ENRICHMENT ; TRANSPLANTATION ; REGENERATION
英文摘要

BACKGROUND & AIMS: Hepatocyte-like cells can be derived from pluripotent stem cells such as embryonic stem (ES) cells, but ES cell-derived hepatic cells with extensive capacity to repopulate liver have not been identified. We aimed to identify and purify ES cell-derived hepatoblast-like progenitor cells and to explore their capacity for liver repopulation in mice after in vitro expansion. METHODS: Unmanipulated mouse ES cells were cultured under defined conditions and allowed to undergo stepwise hepatic differentiation. The derived hepatic cells were examined by morphologic, fluorescence-activated cell sorting, gene expression, and clonal expansion analyses. The capacities of ES cell-derived hepatic progenitor cells to repopulate liver were investigated in mice that were deficient in fumarylacetoacetate hydrolase (Fah) (a model of liver injury). RESULTS: Mouse ES cells were induced to differentiate into a population that contained hepatic progenitor cells; this population included cells that expressed epithelial cell adhesion molecule (EpCAM) but did not express c-Kit. Clonal hepatic progenitors that arose from single c-Kit(-)EpCAM(+) cells could undergo long-term expansion and maintain hepatoblast-like characteristics. Enriched c-Kit(-)EpCAM(+) cells and clonally expanded hepatic progenitor cells repopulated the livers of Fah-deficient mice without inducing tumorigenesis. CONCLUSIONS: ES cell-derived c-Kit(-)EpCAM(+) cells contain a population of hepatoblast-like progenitor cells that can repopulate livers of mice.

语种英语
WOS记录号WOS:000284843600053
项目编号30671044 ; 30623003 ; 30801115 ; 30901449 ; 2006CB943901 ; 2007CB947102 ; 2007CB947903 ; 2009CB941100 ; 2010CB945602 ; 2006AA02Z474 ; KSCX2-YW-R-49 ; DK074561 ; AI065565
资助机构National Natural Science Foundation of China ; National Key Basic Research and Development Program of China ; Chinese National 863 Plan Project ; Chinese Academy of Sciences ; National Institutes of Health
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55249
专题北京大学基础医学院_北京大学干细胞研究中心
作者单位1.Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China
2.Chinese Acad Sci, Grad Sch, Shanghai 200031, Peoples R China
3.Second Mil Med Univ, Dept Cell Biol, Shanghai 200433, Peoples R China
4.Second Mil Med Univ, Sch Pharm, Dept Pharmaceut, Shanghai 200433, Peoples R China
5.Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN USA
6.Univ Vermont, Dept Med, Burlington, VT USA
7.Chinese Acad Sci, Inst Biophys, Beijing 100080, Peoples R China
8.Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Shanghai 200433, Peoples R China
9.Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Beijing 100871, Peoples R China
10.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100871, Peoples R China
11.Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA
12.Univ Minnesota, Stem Cell Inst, Minneapolis, MN USA
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GB/T 7714
Li, Fuming,Liu, Pingyu,Liu, Changcheng,et al. Hepatoblast-Like Progenitor Cells Derived From Embryonic Stem Cells Can Repopulate Livers of Mice[J]. GASTROENTEROLOGY,2010,139(6):2158-U495.
APA Li, Fuming.,Liu, Pingyu.,Liu, Changcheng.,Xiang, Dao.,Deng, Li.,...&Wang, Xin.(2010).Hepatoblast-Like Progenitor Cells Derived From Embryonic Stem Cells Can Repopulate Livers of Mice.GASTROENTEROLOGY,139(6),2158-U495.
MLA Li, Fuming,et al."Hepatoblast-Like Progenitor Cells Derived From Embryonic Stem Cells Can Repopulate Livers of Mice".GASTROENTEROLOGY 139.6(2010):2158-U495.
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