IR@PKUHSC  > 北京大学药学院  > 化学生物学系
学科主题药学
Copper ions inactivate S-adenosylhomocysteine hydrolase
Chen, JJ; Liu, QY; Yang, XD; Wang, K
关键词Copper S-adenosyl-l-homocysteine Hydrolase Biological Transmethlation
刊名CHINESE SCIENCE BULLETIN
2002-07-01
47期:14页:1176-1179
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]MECHANISM ; BINDING ; LIVER ; HOMOCYSTEINE ; ADENOSINE ; KINETICS
英文摘要

S-adenosylhomocysteine (AdoHcy) hydrolase is an enzyme that regulates biomethylation and some other physiological processes. Recombinant AdoHcy hydrolase was overexpressed in E. coli JM109 and. purified with ion exchange and gel filtration chromatographies. The effects of copper ions (Cu2+) on the activity of AdoHey hydrolase were investigated and the results showed that Cu2+ inhibited the enzyme′s activity by a concentration and time-dependent process. The inhibition constant (K-i) and the apparent rate constant (k(app)) were calculated to be (14 +/- 4) nmol (.) L-1 and (1.08 +/- 0.15) min(-1), respectively. The existence of the natural substrate Ado could to some extent prevent Cu2+ from inactivating the enzyme, suggesting that copper ions possibly could compete with the natural substrate on enzyme′s substrate binding site. Further studies on the mechanism of inhibition are being carried out.

语种英语
WOS记录号WOS:000176841000008
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55266
专题北京大学药学院_化学生物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, Natl Res Labs Nat & Biomimet Drugs, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Chen, JJ,Liu, QY,Yang, XD,et al. Copper ions inactivate S-adenosylhomocysteine hydrolase[J]. CHINESE SCIENCE BULLETIN,2002,47(14):1176-1179.
APA Chen, JJ,Liu, QY,Yang, XD,&Wang, K.(2002).Copper ions inactivate S-adenosylhomocysteine hydrolase.CHINESE SCIENCE BULLETIN,47(14),1176-1179.
MLA Chen, JJ,et al."Copper ions inactivate S-adenosylhomocysteine hydrolase".CHINESE SCIENCE BULLETIN 47.14(2002):1176-1179.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Chen, JJ]的文章
[Liu, QY]的文章
[Yang, XD]的文章
百度学术
百度学术中相似的文章
[Chen, JJ]的文章
[Liu, QY]的文章
[Yang, XD]的文章
必应学术
必应学术中相似的文章
[Chen, JJ]的文章
[Liu, QY]的文章
[Yang, XD]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。