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学科主题: 临床医学
题名:
Electrical stimulation does not enhance nerve regeneration if delayed after sciatic nerve injury: the role of fibrosis
作者: Han, Na; Xu, Chun-gui; Wang, Tian-bing; Kou, Yu-hui; Yin, Xiao-feng; Zhang, Pei-xun; Xue, Feng
关键词: nerve regeneration ; peripheral nerve injury ; electrical stimulation ; bioabsorbable conduit ; delayed repair ; fibroblast ; collagen fibers ; transforming growth factor beta ; Masson staining ; neuroprotection ; immunohistochemistry ; NSFC grants ; neural regeneration
刊名: NEURAL REGENERATION RESEARCH
发表日期: 2015
DOI: 10.4103/1673-5374.150714
卷: 10, 期:1, 页:90-94
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Neurosciences
研究领域[WOS]: Cell Biology ; Neurosciences & Neurology
关键词[WOS]: RAT FEMORAL MOTONEURONS ; PERIPHERAL-NERVE ; NEUROTROPHIC FACTOR ; GENE-EXPRESSION ; MOTOR-NEURONS ; IN-VIVO ; PROMOTES ; SPECIFICITY ; SPEED ; BDNF
英文摘要:

Electrical stimulation has been shown to accelerate and enhance nerve regeneration in sensory and motor neurons after injury, but there is little evidence that focuses on the varying degrees of fibrosis in the delayed repair of peripheral nerve tissue. In this study, a rat model of sciatic nerve transection injury was repaired with a biodegradable conduit at 1 day, 1 week, 1 month and 2 months after injury, when the rats were divided into two subgroups. In the experimental group, rats were treated with electrical stimuli of frequency of 20 Hz, pulse width 100 ms and direct current voltage of 3 V; while rats in the control group received no electrical stimulation after the conduit operation. Histological results showed that stained collagen fibers comprised less than 20% of the total operated area in the two groups after delayed repair at both 1 day and 1 week but after longer delays, the collagen fiber area increased with the time after injury. Immunohistochemical staining revealed that the expression level of transforming growth factor beta (an indicator of tissue fibrosis) decreased at both 1 day and 1 week after delayed repair but increased at both 1 and 2 months after delayed repair. These findings indicate that if the biodegradable conduit repair combined with electrical stimulation is delayed, it results in a poor outcome following sciatic nerve injury. One month after injury, tissue degeneration and distal fibrosis are apparent and are probably the main reason why electrical stimulation fails to promote nerve regeneration after delayed repair.

语种: 英语
所属项目编号: 2014CB542206 ; IRT1201 ; 31271284 ; 31171150 ; 81171146 ; 30971526 ; 31100860 ; 31040043 ; 31440055 ; 7142164 ; BMU20110270
项目资助者: National Program on Key Basic Research Project of China (973 Program) ; Program for Innovative Research Team in University of Ministry of Education of China ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing of China ; Program for New Century Excellent Talents in University of Ministry of Education of China
WOS记录号: WOS:000350098100023
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55267
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Dept Trauma & Orthoped, Beijing 100871, Peoples R China

Recommended Citation:
Han, Na,Xu, Chun-gui,Wang, Tian-bing,et al. Electrical stimulation does not enhance nerve regeneration if delayed after sciatic nerve injury: the role of fibrosis[J]. NEURAL REGENERATION RESEARCH,2015,10(1):90-94.
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