IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Electrical stimulation does not enhance nerve regeneration if delayed after sciatic nerve injury: the role of fibrosis
Han, Na; Xu, Chun-gui; Wang, Tian-bing; Kou, Yu-hui; Yin, Xiao-feng; Zhang, Pei-xun; Xue, Feng
关键词Nerve Regeneration Peripheral Nerve Injury Electrical Stimulation Bioabsorbable Conduit Delayed Repair Fibroblast Collagen Fibers Transforming Growth Factor Beta Masson Staining Neuroprotection Immunohistochemistry Nsfc Grants Neural Regeneration
刊名NEURAL REGENERATION RESEARCH
2015
DOI10.4103/1673-5374.150714
10期:1页:90-94
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Neurosciences
资助者National Program on Key Basic Research Project of China (973 Program) ; Program for Innovative Research Team in University of Ministry of Education of China ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing of China ; Program for New Century Excellent Talents in University of Ministry of Education of China ; National Program on Key Basic Research Project of China (973 Program) ; Program for Innovative Research Team in University of Ministry of Education of China ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing of China ; Program for New Century Excellent Talents in University of Ministry of Education of China
研究领域[WOS]Cell Biology ; Neurosciences & Neurology
关键词[WOS]RAT FEMORAL MOTONEURONS ; PERIPHERAL-NERVE ; NEUROTROPHIC FACTOR ; GENE-EXPRESSION ; MOTOR-NEURONS ; IN-VIVO ; PROMOTES ; SPECIFICITY ; SPEED ; BDNF
英文摘要

Electrical stimulation has been shown to accelerate and enhance nerve regeneration in sensory and motor neurons after injury, but there is little evidence that focuses on the varying degrees of fibrosis in the delayed repair of peripheral nerve tissue. In this study, a rat model of sciatic nerve transection injury was repaired with a biodegradable conduit at 1 day, 1 week, 1 month and 2 months after injury, when the rats were divided into two subgroups. In the experimental group, rats were treated with electrical stimuli of frequency of 20 Hz, pulse width 100 ms and direct current voltage of 3 V; while rats in the control group received no electrical stimulation after the conduit operation. Histological results showed that stained collagen fibers comprised less than 20% of the total operated area in the two groups after delayed repair at both 1 day and 1 week but after longer delays, the collagen fiber area increased with the time after injury. Immunohistochemical staining revealed that the expression level of transforming growth factor beta (an indicator of tissue fibrosis) decreased at both 1 day and 1 week after delayed repair but increased at both 1 and 2 months after delayed repair. These findings indicate that if the biodegradable conduit repair combined with electrical stimulation is delayed, it results in a poor outcome following sciatic nerve injury. One month after injury, tissue degeneration and distal fibrosis are apparent and are probably the main reason why electrical stimulation fails to promote nerve regeneration after delayed repair.

语种英语
所属项目编号2014CB542206 ; IRT1201 ; 31271284 ; 31171150 ; 81171146 ; 30971526 ; 31100860 ; 31040043 ; 31440055 ; 7142164 ; BMU20110270
资助者National Program on Key Basic Research Project of China (973 Program) ; Program for Innovative Research Team in University of Ministry of Education of China ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing of China ; Program for New Century Excellent Talents in University of Ministry of Education of China ; National Program on Key Basic Research Project of China (973 Program) ; Program for Innovative Research Team in University of Ministry of Education of China ; National Natural Science Foundation of China ; Natural Science Foundation of Beijing of China ; Program for New Century Excellent Talents in University of Ministry of Education of China
WOS记录号WOS:000350098100023
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55267
专题北京大学第二临床医学院
作者单位Peking Univ, Peoples Hosp, Dept Trauma & Orthoped, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Han, Na,Xu, Chun-gui,Wang, Tian-bing,et al. Electrical stimulation does not enhance nerve regeneration if delayed after sciatic nerve injury: the role of fibrosis[J]. NEURAL REGENERATION RESEARCH,2015,10(1):90-94.
APA Han, Na.,Xu, Chun-gui.,Wang, Tian-bing.,Kou, Yu-hui.,Yin, Xiao-feng.,...&Xue, Feng.(2015).Electrical stimulation does not enhance nerve regeneration if delayed after sciatic nerve injury: the role of fibrosis.NEURAL REGENERATION RESEARCH,10(1),90-94.
MLA Han, Na,et al."Electrical stimulation does not enhance nerve regeneration if delayed after sciatic nerve injury: the role of fibrosis".NEURAL REGENERATION RESEARCH 10.1(2015):90-94.
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