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学科主题: 临床医学
题名:
High Prevalence and Molecular Analysis of Macrolide-Nonsusceptible Moraxella catarrhalis Isolated from Nasopharynx of Healthy Children in China
作者: Liu, Yali1; Zhao, Chunjiang1,2; Zhang, Feifei2; Chen, Hongbin1; Chen, Minjun1; Wang, Hui1,2
刊名: MICROBIAL DRUG RESISTANCE
发表日期: 2012-08-01
DOI: 10.1089/mdr.2011.0175
卷: 18, 期:4, 页:417-426
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
研究领域[WOS]: Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
关键词[WOS]: RESPIRATORY-TRACT INFECTIONS ; SUSCEPTIBLE STREPTOCOCCUS-PNEUMONIAE ; IN-VITRO ACTIVITIES ; 23S RIBOSOMAL-RNA ; HAEMOPHILUS-INFLUENZAE ; ANTIMICROBIAL SUSCEPTIBILITY ; ANTIBIOTIC-RESISTANCE ; GENERAL-PRACTICE ; UNITED-STATES ; CLARITHROMYCIN
英文摘要:

Three hundred eighty-three isolates of Moraxella catarrhalis were collected from healthy children aged less than 2 years in China and assessed for antimicrobial resistance. We found that 92.2% (n = 353) produced a beta-lactamase. Nonsusceptibility rates to erythromycin and azithromycin, determined using Clinical Laboratory Standards Institute (CLSI) breakpoints, were 40.3% and 22.5%, respectively; nonsusceptibility rates determined using pharmacokinetics/pharmacodynamics breakpoints, however, were 59% and 60.1%. The minimal inhibitory concentration (MIC)(90) values were > 256 mu g/ml. Nonsusceptibility rates varied by region from 9.7% in Dongguan to 75.9% in Jinan. Further, concomitant resistance to beta-lactam antibiotics was also observed. Pulsed-field gel electrophoresis analysis of 27/37 high-level macrolide-resistant M. catarrhalis isolates showed that closely related pulsotypes dominated, with a total of 11 different pulsotypes being observed. The closely related pulsotypes were observed in isolates originating from all six Chinese cities investigated, possibly as a consequence of the mobility of the Chinese population. Sixteen patterns of 23S rRNA mutations were found among 97 selected isolates using polymerase chain reaction and sequencing, but no known ermA, ermB, mefA, or mefE genes could be detected. Mutations Lambda 2982T and Lambda 2796T in 23S rRNA were related to high-level macrolide resistance (MICs ranging from 24 to > 256 mu g/ml), while an Lambda 2983T mutation was associated with low-level macrolide resistance (MICs ranging from 0.19 to 16 mu g/ml).

语种: 英语
WOS记录号: WOS:000306742200011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55279
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Peking Union Med Coll, Dept Clin Lab, Beijing 100730, Peoples R China
2.Peking Univ, Peking Univ Peoples Hosp, Dept Clin Lab, Beijing 100871, Peoples R China

Recommended Citation:
Liu, Yali,Zhao, Chunjiang,Zhang, Feifei,et al. High Prevalence and Molecular Analysis of Macrolide-Nonsusceptible Moraxella catarrhalis Isolated from Nasopharynx of Healthy Children in China[J]. MICROBIAL DRUG RESISTANCE,2012,18(4):417-426.
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