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学科主题基础医学
Effects of Hypoxic Preconditioning on Synaptic Ultrastructure in Mice
Liu, Yi1,2,3; Sun, Zhishan1,4; Sun, Shufeng5; Duan, Yunxia1; Shi, Jingfei1; Qi, Zhifeng1; Meng, Ran1; Sun, Yongxin1; Zeng, Xianwei4; Chui, Dehua6,7; Ji, Xunming1
关键词hypoxic preconditioning synaptic curvature presynaptic mitochondria
刊名SYNAPSE
2015
DOI10.1002/syn.21777
69期:1页:7-14
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]LONG-TERM POTENTIATION ; CEREBRAL-ISCHEMIA ; DENTATE GYRUS ; PLASTICITY ; MORPHOLOGY ; SYNAPSES ; INJURY
英文摘要

Hypoxic preconditioning (HPC) elicits resistance to more drastic subsequent insults, which potentially provide neuroprotective therapeutic strategy, but the underlying mechanisms remain to be fully elucidated. Here, we examined the effects of HPC on synaptic ultrastructure in olfactory bulb of mice. Mice underwent up to five cycles of repeated HPC treatments, and hypoxic tolerance was assessed with a standard gasp reflex assay. As expected, HPC induced an increase in tolerance time. To assess synaptic responses, Western blots were used to quantify protein levels of representative markers for glia, neuron, and synapse, and transmission electron microscopy was used to examine synaptic ultrastructure and mitochondrial density. HPC did not significantly alter the protein levels of astroglial marker (GFAP), neuron-specific markers (GAP43, Tuj-1, and OMP), synaptic number markers (synaptophysin and SNAP25) or the percentage of excitatory synapses versus inhibitory synapses. However, HPC significantly affected synaptic curvature and the percentage of synapses with presynaptic mitochondria, which showed concomitant change pattern. These findings demonstrate that HPC is associated with changes in synaptic ultrastructure. (C) 2014 Wiley Periodicals, Inc.

语种英语
WOS记录号WOS:000345271100002
项目编号81200915
资助机构National Natural Science Foundation of China
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55297
专题北京大学基础医学院_神经生物学系
作者单位1.Capital Med Univ, Xuanwu Hosp, CAJIN, Beijing 100053, Peoples R China
2.Harvard Univ, Sch Med, Dept Radiol, Neuroprotect Res Lab,Massachusetts Gen Hosp, Boston, MA 02115 USA
3.Harvard Univ, Sch Med, Dept Neurol, Neuroprotect Res Lab,Massachusetts Gen Hosp, Boston, MA 02115 USA
4.Weifang Med Coll, Affiliated Hosp, Dept Neurosurg, Weifang, Peoples R China
5.Chinese Acad Sci, Inst Biophys, Ctr Biol Electron Microscopy, Beijing 100101, Peoples R China
6.Peking Univ, Hlth Sci Ctr, Neurosci Res Inst, Beijing 100191, Peoples R China
7.Peking Univ, Hlth Sci Ctr, Minist Publ Hlth, Dept Neurobiol,Key Lab Neurosci,Minist Educ, Beijing 100191, Peoples R China
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GB/T 7714
Liu, Yi,Sun, Zhishan,Sun, Shufeng,et al. Effects of Hypoxic Preconditioning on Synaptic Ultrastructure in Mice[J]. SYNAPSE,2015,69(1):7-14.
APA Liu, Yi.,Sun, Zhishan.,Sun, Shufeng.,Duan, Yunxia.,Shi, Jingfei.,...&Ji, Xunming.(2015).Effects of Hypoxic Preconditioning on Synaptic Ultrastructure in Mice.SYNAPSE,69(1),7-14.
MLA Liu, Yi,et al."Effects of Hypoxic Preconditioning on Synaptic Ultrastructure in Mice".SYNAPSE 69.1(2015):7-14.
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