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学科主题: 基础医学
题名:
Effects of Hypoxic Preconditioning on Synaptic Ultrastructure in Mice
作者: Liu, Yi1,2,3; Sun, Zhishan1,4; Sun, Shufeng5; Duan, Yunxia1; Shi, Jingfei1; Qi, Zhifeng1; Meng, Ran1; Sun, Yongxin1; Zeng, Xianwei4; Chui, Dehua6,7; Ji, Xunming1
关键词: hypoxic preconditioning ; synaptic curvature ; presynaptic mitochondria
刊名: SYNAPSE
发表日期: 2015
DOI: 10.1002/syn.21777
卷: 69, 期:1, 页:7-14
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: LONG-TERM POTENTIATION ; CEREBRAL-ISCHEMIA ; DENTATE GYRUS ; PLASTICITY ; MORPHOLOGY ; SYNAPSES ; INJURY
英文摘要:

Hypoxic preconditioning (HPC) elicits resistance to more drastic subsequent insults, which potentially provide neuroprotective therapeutic strategy, but the underlying mechanisms remain to be fully elucidated. Here, we examined the effects of HPC on synaptic ultrastructure in olfactory bulb of mice. Mice underwent up to five cycles of repeated HPC treatments, and hypoxic tolerance was assessed with a standard gasp reflex assay. As expected, HPC induced an increase in tolerance time. To assess synaptic responses, Western blots were used to quantify protein levels of representative markers for glia, neuron, and synapse, and transmission electron microscopy was used to examine synaptic ultrastructure and mitochondrial density. HPC did not significantly alter the protein levels of astroglial marker (GFAP), neuron-specific markers (GAP43, Tuj-1, and OMP), synaptic number markers (synaptophysin and SNAP25) or the percentage of excitatory synapses versus inhibitory synapses. However, HPC significantly affected synaptic curvature and the percentage of synapses with presynaptic mitochondria, which showed concomitant change pattern. These findings demonstrate that HPC is associated with changes in synaptic ultrastructure. (C) 2014 Wiley Periodicals, Inc.

语种: 英语
所属项目编号: 81200915
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000345271100002
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55297
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Capital Med Univ, Xuanwu Hosp, CAJIN, Beijing 100053, Peoples R China
2.Harvard Univ, Sch Med, Dept Radiol, Neuroprotect Res Lab,Massachusetts Gen Hosp, Boston, MA 02115 USA
3.Harvard Univ, Sch Med, Dept Neurol, Neuroprotect Res Lab,Massachusetts Gen Hosp, Boston, MA 02115 USA
4.Weifang Med Coll, Affiliated Hosp, Dept Neurosurg, Weifang, Peoples R China
5.Chinese Acad Sci, Inst Biophys, Ctr Biol Electron Microscopy, Beijing 100101, Peoples R China
6.Peking Univ, Hlth Sci Ctr, Neurosci Res Inst, Beijing 100191, Peoples R China
7.Peking Univ, Hlth Sci Ctr, Minist Publ Hlth, Dept Neurobiol,Key Lab Neurosci,Minist Educ, Beijing 100191, Peoples R China

Recommended Citation:
Liu, Yi,Sun, Zhishan,Sun, Shufeng,et al. Effects of Hypoxic Preconditioning on Synaptic Ultrastructure in Mice[J]. SYNAPSE,2015,69(1):7-14.
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