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IR@PKUHSC  > 北京大学第二临床医学院  > 肝胆外科  > 期刊论文
学科主题: 临床医学
题名:
Expression of cancer-testis antigens in hepatocellular carcinoma
作者: Zhao, Li1; Mou, Dong-Cheng2; Leng, Xi-Sheng1; Peng, Ji-Run1; Wang, Wan-Xiang1; Huang, Lei1; Li, Shu1; Zhu, Ji-Ye1
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2004-07-15
卷: 10, 期:14, 页:2034-2038
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
英文摘要:

AIM: To investigate the expression of cancer-testis (CT) antigens MAGE-1, SSX-1, CTp11 and HCA587 genes in hepatocellular carcinoma (HCC) and the possibility of applying these antigens as targets for specific immunotherapy for HCC.

METHODS: Expression levels of MAGE-1, SSX-1, CTp11 and HCA587 mRNA were detected with reverse transcription polymerase chain reaction (RT-PCR) in HCC tissues and corresponding adjacent non-cancerous tissues from 105 HCC patients, 40 samples of cirrhosis and normal liver tissues. Genes of five samples with positive PCR results were sequenced.

RESULTS: Of 105 HCC tissues, MAGE1, SSX-1, CTp11 and HCA587 mRNA expressions were detectable in 75.2%(79/105), 72.4%(76/105), 62.9%(66/105) and 56.2%(59/105) of HCC samples, respectively. About 93.3%(98/105), 72.4%(76/105), 48.6%(51/105) and 37.1%(39/105) of HCC tissues positively expressed at least one, two, three, and four members of CT antigens, respectively. Conversely, only SSX-1 could be detectable in 2.9%(3/105) of the corresponding adjacent non-HCC tissues in which no metastatic lesion was found. Of the latter 3 patients, biopsy samples far from tumor were obtained in 2 patients and RT-PCR indicated no expression of SSX-1 mRNA in these two samples. In addition, none of 40 samples of cirrhotic and normal liver tissues expressed CT antigen gene mRNA. DNA sequences confirmed that the RT-PCR products were true target cDNA. No relationship was found between expression of CT antigens and clinico pathological indicators such as age, gender, tumor size, degree of tumor differentiation, serum alpha-fetoprotein level and infection of hepatitis B virus or hepatitis C virus (P>0.05).

CONCLUSION: CT antigens genes (MAGE-1, SSX-1, CTp11 and HCA587) are expressed with high percentage and specificity in HCC and their products are promising targets for antigen-specific immunotherapy of HCC. High frequent co-expression of multiple members of CT antigens in HCC provides possibility of polyvalent vaccinations for HCC.

语种: 英语
所属项目编号: 30200271 ; 2001BA703B04
项目资助者: National Natural Science Foundation of China ; National Key Technologies Research and Development Program of China ; Ministry of Health of China ; Center for Human Disease Genomics of Peking University
WOS记录号: WOS:000208097700007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55300
Appears in Collections:北京大学第二临床医学院_肝胆外科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Ctr Hepatobiliary Surg, Beijing 100044, Peoples R China
2.Tsinghua Univ, Affiliated Hosp 1, Dept Gen Surg, Beijing 100016, Peoples R China

Recommended Citation:
Zhao, Li,Mou, Dong-Cheng,Leng, Xi-Sheng,et al. Expression of cancer-testis antigens in hepatocellular carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(14):2034-2038.
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