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Hypermethylation of the CpG Island Spanning From Exon II to Intron III is Associated With Steroidogenic Factor 1 Expression in Stromal Cells of Endometriosis
Xue, Qing2; Zhou, Ying Fang2; Zhu, Sai Nan3; Bulun, Serdar E.1
关键词Sf-1 Endometriosis Dna Methylation Intron Cpg Island
刊名REPRODUCTIVE SCIENCES
2011-11-01
DOI10.1177/1933719111404614
18期:11页:1080-1084
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
资助者Natural Science Foundation of China ; Science Foundation of First Hospital of Beijing University ; NIH/NICHD ; Natural Science Foundation of China ; Science Foundation of First Hospital of Beijing University ; NIH/NICHD
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]GENOME-WIDE ANALYSIS ; TRANSCRIPTION FACTOR ; BINDING-SITES ; AROMATASE EXPRESSION ; RECEPTOR ; METHYLATION ; PROTEIN ; BREAST ; CANCER ; GENE
英文摘要

Endometriosis is an estrogen-dependent disease. Steroidogenic factor 1 (SF-1), a transcription factor, is essential for the activation of multiple steroidogenic genes for estrogen biosynthesis in endometriosis-derived stromal cells. Objective: Unravel the mechanism for differential SF-1 expression in endometrial and endometriotic stromal cells. DESIGN: We identified a novel CpG island in the SF-1 gene, which spans from exon II to intron III. We evaluated the methylation status of this CpG island. PATIENTS: Eutopic endometrium from disease-free participants (n = 8) and the walls of cystic endometriosis lesions of the ovaries (n = 8). None of the patients had received any preoperative hormonal therapy. Stromal cells were isolated from these 2 types of tissues. Results: SF-1 messenger RNA (mRNA) levels in endometriotic stromal cells were significantly higher than those in endometrial stromal cells. Bisulfite sequencing showed strikingly increased methylation in endometriotic cells compared with endometrial cells (P < .001). A strong correlation between mRNA levels and percentage methylation of the exon II/intron III are observed. Specifically, the Pearson correlation coefficient was .98 (P < .001) for this association. Conclusions: We demonstrated that methylation of a coding exon/intron sequence in the SF-1 gene positively regulated its expression in endometriosis, whereas its hypomethylation in normal endometrium was associated with drastically lower SF-1 levels.

语种英语
所属项目编号30973183 ; R37-HD38691
资助者Natural Science Foundation of China ; Science Foundation of First Hospital of Beijing University ; NIH/NICHD ; Natural Science Foundation of China ; Science Foundation of First Hospital of Beijing University ; NIH/NICHD
WOS记录号WOS:000296206300004
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55302
专题北京大学第一临床医学院
作者单位1.Northwestern Univ, Dept Obstet & Gynecol, Div Reprod Endocrinol & Infertil, Feinberg Sch Med, Chicago, IL 60611 USA
2.Beijing Univ, Dept Obstet & Gynecol, Hosp 1, Beijing 100871, Peoples R China
3.Beijing Univ, Dept Biostat, Hosp 1, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Xue, Qing,Zhou, Ying Fang,Zhu, Sai Nan,et al. Hypermethylation of the CpG Island Spanning From Exon II to Intron III is Associated With Steroidogenic Factor 1 Expression in Stromal Cells of Endometriosis[J]. REPRODUCTIVE SCIENCES,2011,18(11):1080-1084.
APA Xue, Qing,Zhou, Ying Fang,Zhu, Sai Nan,&Bulun, Serdar E..(2011).Hypermethylation of the CpG Island Spanning From Exon II to Intron III is Associated With Steroidogenic Factor 1 Expression in Stromal Cells of Endometriosis.REPRODUCTIVE SCIENCES,18(11),1080-1084.
MLA Xue, Qing,et al."Hypermethylation of the CpG Island Spanning From Exon II to Intron III is Associated With Steroidogenic Factor 1 Expression in Stromal Cells of Endometriosis".REPRODUCTIVE SCIENCES 18.11(2011):1080-1084.
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