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学科主题: 临床医学
题名:
Osthole ameliorates renal ischemia-reperfusion injury in rats
作者: Zheng, Yi1; Lu, Min2; Ma, Lulin1; Zhang, Shudong1; Qiu, Min1; Wang, Yunpeng1
关键词: Kidney ; Ischemia-reperfusion injury ; Osthole ; Apoptosis ; Oxidative stress
刊名: JOURNAL OF SURGICAL RESEARCH
发表日期: 2013-07-01
DOI: 10.1016/j.jss.2013.01.008
卷: 183, 期:1, 页:347-354
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Surgery
研究领域[WOS]: Surgery
关键词[WOS]: INDUCED FATTY LIVER ; ISCHEMIA/REPERFUSION INJURY ; OXIDATIVE STRESS ; CYTOCHROME-C ; FAILURE ; APOPTOSIS ; MICE ; KIDNEY ; CYTOTOXICITY ; INHIBITION
英文摘要:

Background: Renal ischemia-reperfusion (I/R) injury is a major cause of acute kidney injury. The pathogenetic mechanisms underlying I/R injury involve oxidative stress and apoptosis. Osthole, a natural coumarin derivative, has been reported to possess antioxidant and antiapoptotic activities. This study aimed to investigate the potential effects of osthole on renal I/R injury in an in vivo rat model.

Materials and methods: We induced renal I/R injury by clamping the left renal artery for 45 min followed by reperfusion, along with a contralateral nephrectomy. We randomly assigned 54 rats to three groups (18 rats/group): sham-operated, vehicle-treated I/R, and osthole-treated I/R. We treated rats intraperitoneally with osthole (40 mg/kg) or vehicle (40 mg/kg) 30 min before renal ischemia. We harvested serum and kidneys at 1, 6, and 24 h after reperfusion. Renal function and histological changes were assessed. We also determined markers of oxidative stress and cell apoptosis in kidneys.

Results: Osthole treatment significantly attenuated renal dysfunction and histologic damage induced by I/R injury. The I/R-induced elevation in kidney malondialdehyde level decreased, whereas reduced kidney superoxide dismutase and catalase activities were markedly increased. Moreover, osthole-treated rats had a dramatic decrease in apoptotic tubular cells, along with a decrease in caspase-3 and an increase in the Bcl-2/Bax ratio.

Conclusions: Osthole treatment protects murine kidney from renal I/R injury by suppressing oxidative stress and cell apoptosis. Thus, osthole may represent a novel practical strategy to prevent renal I/R injury. (C) 2013 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30950018 ; 7113174
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000320599600051
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55303
Appears in Collections:北京大学第三临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Urol, Beijing 100191, Peoples R China
2.Peking Univ, Dept Pathol, Sch Basic Med Sci, Beijing 100191, Peoples R China

Recommended Citation:
Zheng, Yi,Lu, Min,Ma, Lulin,et al. Osthole ameliorates renal ischemia-reperfusion injury in rats[J]. JOURNAL OF SURGICAL RESEARCH,2013,183(1):347-354.
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