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学科主题: 药学
题名:
Separation and detection of minor constituents in herbal medicines using a combination of heart-cutting and comprehensive two-dimensional liquid chromatography
作者: Qiao, Xue1; Song, Wei1; Ji, Shuai1; Li, Yan-jiao1; Wang, Yuan1; Li, Ru1; An, Rong2; Guo, De-an1; Ye, Min1
关键词: HC-2DLC ; Chinese herbal medicine ; Pueraria lobata ; Pueraria thomsonii ; Synchronized gradient elution
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2014-10-03
DOI: 10.1016/j.chroma.2014.08.038
卷: 1362, 页:157-167
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: TRADITIONAL CHINESE MEDICINE ; TANDEM MASS-SPECTROMETRY ; PUERARIA-LOBATA ; ELECTROSPRAY-IONIZATION ; ISOFLAVONE GLYCOSIDES ; GLYCYRRHIZA-URALENSIS ; PEAK-CAPACITY ; PANAX-GINSENG ; IDENTIFICATION ; COLUMN
英文摘要:

Herbal medicines contain a large number of minor constituents, which could contribute to their therapeutic effects and provide valuable lead compounds for drug discovery. However, to explore minor constituents from complicated herbal extracts is usually laborious and time-consuming. In order to discover minor novel herbal constituents efficiently, we combined heart-cutting and comprehensive two-dimensional liquid chromatography (HC-2DLC) to remove major components from herbal extracts, and then characterized the minor ones by mass spectrometry. This strategy was employed to analyze Pueraria lobata and Pueraria thomsonii, the roots of which are used as the Chinese herbal medicine Ge-Gen. Five major compounds in Ge-Gen extract were removed by on-line heart-cutting, and the minor compounds were separated on an RP x RP 2DLC system (1D, Acquity CSH C-18, 2.1 x 100 mm, 1.7 mu m; 2D, Poroshell Phenyl-Hexyl, 3.0 x 50 mm, 2.7 mu m). A synchronized gradient elution program was used to improve chromatographic resolution of the second dimension. By using this 2DLC system, a total of 271 and 254 peaks were separated in P. lobata and P. thomsonii within 35 min, respectively. The practical and effective peak capacity was 1593 and 677, respectively, and the orthogonality was around 70%. Structures of 12 selected compounds were tentatively characterized by mass spectrometry, and 9 of them were discovered from Ge-Gen for the first time. Contents of these minor compounds in Ge-Gen were preliminarily determined to be 0.01-0.1% (w/w). The HC-2DLC/MS system is a powerful and convenient tool to explore minor novel chemical constituents from complex herbal extracts. (C) 2014 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 81222054 ; 81470172 ; NCET-11-0019
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Chinese Ministry of Education
WOS记录号: WOS:000342530600016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55306
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Agilent Technol, Beijing 100102, Peoples R China

Recommended Citation:
Qiao, Xue,Song, Wei,Ji, Shuai,et al. Separation and detection of minor constituents in herbal medicines using a combination of heart-cutting and comprehensive two-dimensional liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1362:157-167.
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