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Separation and detection of minor constituents in herbal medicines using a combination of heart-cutting and comprehensive two-dimensional liquid chromatography
Qiao, Xue1; Song, Wei1; Ji, Shuai1; Li, Yan-jiao1; Wang, Yuan1; Li, Ru1; An, Rong2; Guo, De-an1; Ye, Min1
关键词Hc-2dlc Chinese Herbal Medicine Pueraria Lobata Pueraria Thomsonii Synchronized Gradient Elution
刊名JOURNAL OF CHROMATOGRAPHY A
2014-10-03
DOI10.1016/j.chroma.2014.08.038
1362页:157-167
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TRADITIONAL CHINESE MEDICINE ; TANDEM MASS-SPECTROMETRY ; PUERARIA-LOBATA ; ELECTROSPRAY-IONIZATION ; ISOFLAVONE GLYCOSIDES ; GLYCYRRHIZA-URALENSIS ; PEAK-CAPACITY ; PANAX-GINSENG ; IDENTIFICATION ; COLUMN
英文摘要

Herbal medicines contain a large number of minor constituents, which could contribute to their therapeutic effects and provide valuable lead compounds for drug discovery. However, to explore minor constituents from complicated herbal extracts is usually laborious and time-consuming. In order to discover minor novel herbal constituents efficiently, we combined heart-cutting and comprehensive two-dimensional liquid chromatography (HC-2DLC) to remove major components from herbal extracts, and then characterized the minor ones by mass spectrometry. This strategy was employed to analyze Pueraria lobata and Pueraria thomsonii, the roots of which are used as the Chinese herbal medicine Ge-Gen. Five major compounds in Ge-Gen extract were removed by on-line heart-cutting, and the minor compounds were separated on an RP x RP 2DLC system (1D, Acquity CSH C-18, 2.1 x 100 mm, 1.7 mu m; 2D, Poroshell Phenyl-Hexyl, 3.0 x 50 mm, 2.7 mu m). A synchronized gradient elution program was used to improve chromatographic resolution of the second dimension. By using this 2DLC system, a total of 271 and 254 peaks were separated in P. lobata and P. thomsonii within 35 min, respectively. The practical and effective peak capacity was 1593 and 677, respectively, and the orthogonality was around 70%. Structures of 12 selected compounds were tentatively characterized by mass spectrometry, and 9 of them were discovered from Ge-Gen for the first time. Contents of these minor compounds in Ge-Gen were preliminarily determined to be 0.01-0.1% (w/w). The HC-2DLC/MS system is a powerful and convenient tool to explore minor novel chemical constituents from complex herbal extracts. (C) 2014 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000342530600016
项目编号81222054 ; 81470172 ; NCET-11-0019
资助机构National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Chinese Ministry of Education
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55306
专题北京大学药学院
北京大学药学院_天然药物学系
北京大学第三临床医学院_护理部
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Agilent Technol, Beijing 100102, Peoples R China
推荐引用方式
GB/T 7714
Qiao, Xue,Song, Wei,Ji, Shuai,et al. Separation and detection of minor constituents in herbal medicines using a combination of heart-cutting and comprehensive two-dimensional liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1362:157-167.
APA Qiao, Xue.,Song, Wei.,Ji, Shuai.,Li, Yan-jiao.,Wang, Yuan.,...&Ye, Min.(2014).Separation and detection of minor constituents in herbal medicines using a combination of heart-cutting and comprehensive two-dimensional liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1362,157-167.
MLA Qiao, Xue,et al."Separation and detection of minor constituents in herbal medicines using a combination of heart-cutting and comprehensive two-dimensional liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1362(2014):157-167.
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