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学科主题: 基础医学
题名:
Enhanced Survival of Melanopsin-expressing Retinal Ganglion Cells After Injury is Associated with the PI3 K/Akt Pathway
作者: Li, Suk-Yee1,2,3; Yau, Suk-Yu1,2; Chen, Bai-Yu1,2,4; Tay, David K.1,2; Lee, Vincent W. H.1,5; Pu, Ming-Liang4; Chan, Henry H. L.6; So, Kwok-Fai1,2,7
关键词: Melanopsin ; Retinal ganglion cell ; Optic nerve injury ; Glaucoma
刊名: CELLULAR AND MOLECULAR NEUROBIOLOGY
发表日期: 2008-12-01
DOI: 10.1007/s10571-008-9286-x
卷: 28, 期:8, 页:1095-1107
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Neurosciences
研究领域[WOS]: Cell Biology ; Neurosciences & Neurology
关键词[WOS]: ELEVATED INTRAOCULAR-PRESSURE ; OCULAR HYPERTENSION ; OPTIC-NERVE ; KINASE ACTIVATION ; ADULT-RATS ; DEATH ; AXOTOMY ; REGENERATION ; APOPTOSIS ; MODEL
英文摘要:

In the present study, we studied the factors that contribute to the injury-resistant property of melanopsin-expressing retinal ganglion cells (mRGCs). Since phosphatidylinositol-3 kinase (PI3 K)/Akt signaling pathway is one of the well-known pathways for neuronal cell survival, we investigated the survival of mRGCs by applying the PI3 K/Akt specific inhibitors after injury. Two injury models, unilateral optic nerve transection and ocular hypertension, were adopted using Sprague-Dawley rats. Inhibitors of PI3 K/Akt were injected intravitreally following injuries to inhibit the PI3 K/Akt signaling pathway. Retinas were dissected after designated survival time, immunohistochemistry was carried out to visualize the mRGCs using melanopsin antibody and the number of mRGCs was counted. Co-expression of melanopsin and phospho-Akt (pAkt) was also examined. Compared to the survival of non-melanopsin-expressing RGCs, mRGCs showed a marked resistance to injury and co-expressed pAkt. Application of PI3 K/Akt inhibitors decreased the survival of mRGCs after injury. Our previous study has shown that mRGC are less susceptible to injury following the induction of ocular hypertension. In this study, we report that mRGCs were injury-resistant to a more severe type of injury, the optic nerve transection. More importantly, the PI3 K/Akt pathway was found to play a role in maintaining the survival of mRGCs after injury.

语种: 英语
所属项目编号: BQ-867
项目资助者: Hong Kong Charitable Foundation ; Hong Kong Polytechnic University
WOS记录号: WOS:000261329500007
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55335
Appears in Collections:基础医学院_解剖学与组织胚胎学系_期刊论文

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作者单位: 1.Univ Hong Kong, Li Ka Shing Fac Med, Dept Anat, Pokfulam, Hong Kong, Peoples R China
2.Univ Hong Kong, State Key Lab Brain & Cognit Sci, Pokfulam, Hong Kong, Peoples R China
3.Univ Hong Kong, Li Ka Shing Fac Med, Inst Eye, Pokfulam, Hong Kong, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Anat, Beijing 100871, Peoples R China
5.Hong Kong Adventist Hosp, Mr & Mrs Tung Kay Fung Ophthalm Laser Ctr, Hong Kong, Peoples R China
6.Hong Kong Polytech Univ, Fac Hlth & Social Sci, Sch Optometry, Hum Hom, Hong Kong, Peoples R China
7.Univ Hong Kong, Res Ctr Heart Brain Hormone & Healthy Aging, Pokfulam, Hong Kong, Peoples R China

Recommended Citation:
Li, Suk-Yee,Yau, Suk-Yu,Chen, Bai-Yu,et al. Enhanced Survival of Melanopsin-expressing Retinal Ganglion Cells After Injury is Associated with the PI3 K/Akt Pathway[J]. CELLULAR AND MOLECULAR NEUROBIOLOGY,2008,28(8):1095-1107.
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