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学科主题: 口腔医学
题名:
Synovial TRPV1 is upregulated by 17-beta-estradiol and involved in allodynia of inflamed temporomandibular joints in female rats
作者: Wu, Yu-Wei1,2; Hao, Ting1,2; Kou, Xiao-Xing1,2; Gan, Ye-Hua1,2; Ma, Xu-Chen2
关键词: Estradiol ; Synoviocytes ; TRPV1 ; NGF ; COX-2
刊名: ARCHIVES OF ORAL BIOLOGY
发表日期: 2015-09-01
DOI: 10.1016/j.archoralbio.2015.05.011
卷: 60, 期:9, 页:1310-1318
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: NERVE GROWTH-FACTOR ; RHEUMATOID-ARTHRITIS ; CAPSAICIN RECEPTOR ; NEUROTROPHIN RECEPTOR ; INTERNAL DERANGEMENT ; OVARIECTOMIZED RATS ; CATS KNEE ; PAIN ; EXPRESSION ; OSTEOARTHRITIS
英文摘要:

Women with reproductive capability are more likely to suffer from temporomandibular disorders (TMD), with orofacial pain as the most common complaint. In the past, we focused on the role of estradiol in TMD pain through the nervous system. In this study, we explored estradiol′s influence on synoviocyte gene expressions involved in the allodynia of the inflamed TMJ. The influence of 17-beta-estradiol on NGF and TRPV1 expression in TMJ synovium was determined in vivo and in vitro and analyzed by Western blot and real-time PCR. Complete Freund′s adjuvant (CFA) injection into the TMJ was used to induce TMJ arthritis. Capsazepine served as a TRPV1 antagonist. Head withdrawal threshold was examined using a von Frey Anesthesiometer. We observed that estradiol upregulated the expressions of TRPV1 and NGF in a dose-dependent manner. In the primary cultured synoviocytes, TRPV1 was upregulated by lipopolysaccharide (LPS), estradiol, and NGF, while NGF antibodies fully blocked LPS and estradiol-induced upregulation of TRPV1. Activation of TRPV1 in the primary synoviocytes with capsaicin, a TRPV1 agonist, dose-dependently enhanced COX-2 transcription. Moreover, intra-TMJ injection of TRPV1 antagonist, capsazepine, significantly attenuated allodynia of the inflamed TMJ induced by intra-TMJ injection of CFA in female rats. This article presents a possible local mechanism for estradiol that may be involved in TMJ inflammation or pain in the synovial membrane through the pain-related gene TRPV1. This finding could potentially help clinicians understand the sexual dimorphism of TMD pain. (C) 2015 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 30740046 ; 81070849
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000360511700015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55358
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Mol Biol Lab, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Ctr TMD & Orofacial Pain, Sch & Hosp Stomatol, Beijing 100081, Peoples R China

Recommended Citation:
Wu, Yu-Wei,Hao, Ting,Kou, Xiao-Xing,et al. Synovial TRPV1 is upregulated by 17-beta-estradiol and involved in allodynia of inflamed temporomandibular joints in female rats[J]. ARCHIVES OF ORAL BIOLOGY,2015,60(9):1310-1318.
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