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Synovial TRPV1 is upregulated by 17-beta-estradiol and involved in allodynia of inflamed temporomandibular joints in female rats
Wu, Yu-Wei1,2; Hao, Ting1,2; Kou, Xiao-Xing1,2; Gan, Ye-Hua1,2; Ma, Xu-Chen2
关键词Estradiol Synoviocytes Trpv1 Ngf Cox-2
刊名ARCHIVES OF ORAL BIOLOGY
2015-09-01
DOI10.1016/j.archoralbio.2015.05.011
60期:9页:1310-1318
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]NERVE GROWTH-FACTOR ; RHEUMATOID-ARTHRITIS ; CAPSAICIN RECEPTOR ; NEUROTROPHIN RECEPTOR ; INTERNAL DERANGEMENT ; OVARIECTOMIZED RATS ; CATS KNEE ; PAIN ; EXPRESSION ; OSTEOARTHRITIS
英文摘要

Women with reproductive capability are more likely to suffer from temporomandibular disorders (TMD), with orofacial pain as the most common complaint. In the past, we focused on the role of estradiol in TMD pain through the nervous system. In this study, we explored estradiol′s influence on synoviocyte gene expressions involved in the allodynia of the inflamed TMJ. The influence of 17-beta-estradiol on NGF and TRPV1 expression in TMJ synovium was determined in vivo and in vitro and analyzed by Western blot and real-time PCR. Complete Freund′s adjuvant (CFA) injection into the TMJ was used to induce TMJ arthritis. Capsazepine served as a TRPV1 antagonist. Head withdrawal threshold was examined using a von Frey Anesthesiometer. We observed that estradiol upregulated the expressions of TRPV1 and NGF in a dose-dependent manner. In the primary cultured synoviocytes, TRPV1 was upregulated by lipopolysaccharide (LPS), estradiol, and NGF, while NGF antibodies fully blocked LPS and estradiol-induced upregulation of TRPV1. Activation of TRPV1 in the primary synoviocytes with capsaicin, a TRPV1 agonist, dose-dependently enhanced COX-2 transcription. Moreover, intra-TMJ injection of TRPV1 antagonist, capsazepine, significantly attenuated allodynia of the inflamed TMJ induced by intra-TMJ injection of CFA in female rats. This article presents a possible local mechanism for estradiol that may be involved in TMJ inflammation or pain in the synovial membrane through the pain-related gene TRPV1. This finding could potentially help clinicians understand the sexual dimorphism of TMD pain. (C) 2015 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000360511700015
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55358
专题北京大学口腔医学院
北京大学口腔医学院_口腔正畸科
北京大学口腔医学院_第二门诊部
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Mol Biol Lab, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Ctr TMD & Orofacial Pain, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
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Wu, Yu-Wei,Hao, Ting,Kou, Xiao-Xing,et al. Synovial TRPV1 is upregulated by 17-beta-estradiol and involved in allodynia of inflamed temporomandibular joints in female rats[J]. ARCHIVES OF ORAL BIOLOGY,2015,60(9):1310-1318.
APA Wu, Yu-Wei,Hao, Ting,Kou, Xiao-Xing,Gan, Ye-Hua,&Ma, Xu-Chen.(2015).Synovial TRPV1 is upregulated by 17-beta-estradiol and involved in allodynia of inflamed temporomandibular joints in female rats.ARCHIVES OF ORAL BIOLOGY,60(9),1310-1318.
MLA Wu, Yu-Wei,et al."Synovial TRPV1 is upregulated by 17-beta-estradiol and involved in allodynia of inflamed temporomandibular joints in female rats".ARCHIVES OF ORAL BIOLOGY 60.9(2015):1310-1318.
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