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学科主题: 临床医学
题名:
Methylation of CpG islands of p16 associated with progression of primary gastric carcinomas
作者: Luo, DY; Zhang, BZ; Lv, LB; Xiang, SY; Liu, YH; Ji, JF; Deng, DJ
关键词: CpG Islands ; DHPLC ; gastric carcinoma ; methylation ; p16 ; quantification
刊名: LABORATORY INVESTIGATION
发表日期: 2006-06-01
DOI: 10.1038/labinvest.3700415
卷: 86, 期:6, 页:591-598
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pathology
研究领域[WOS]: Research & Experimental Medicine ; Pathology
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; TUMOR-SUPPRESSOR GENE ; PROMOTER HYPERMETHYLATION ; DNA METHYLATION ; HUMAN CANCERS ; LUNG-CANCER ; INACTIVATION ; DISEASE
英文摘要:

Inactivation of p16 by methylation of CpG islands is a frequent early event in gastric carcinogenesis. The positive relationship between p16 methylation and the clinical characteristics of gastric carcinomas (GC) has not been reported to date. In the present study, a DHPLC assay to quantify p16 methylation was established (detection limit by fluorescence detector: 1:255 (Methlyated vs Unmethylated)). The proportion of methylated p16 in the representative samples was confirmed and standardized by clone sequencing. Then, the DHPLC and two regular methylation-specific PCR (MSP) assays were used to detect p16 methylation in 82 paired, resected GCs and their adjacent normal tissues. Results showed that the average proportion of methylated p16 in GCs was significantly higher than that in their adjacent samples (12.90 vs 0.63%; t-test P = 0.005). A much higher proportion of methylated p16 was detected in GCs with metastases (local or distant) than without metastases (14.76 vs 2.61%; t-test P = 0.014). A proportional relationship was observed between clinical stages and positive rates of p16 methylation in GCs and/or adjacent tissues: 27.3, 37.5, and 58.8% (by DHPLC) for stage-I ,-II, - III - IV of GCs, respectively (two-sided Fisher′s exact test P = 0.016). To confirm the data obtained by DHPLC, two MSP primer sets (p16-M and p16-M2) were also used to analyze p16 methylation in the same set of samples simultaneously. Data of MSP assay using the primer set p16-M2, but not p16-M, correlated with that of DHPLC. These results imply that the primer set p16-M2 might be more suitable than p16-M to detect p16 methylation in gastric tissues. In conclusion, the present data indicates that p16 methylation correlates with progression of GCs significantly.

语种: 英语
WOS记录号: WOS:000237703500006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55360
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Beijing Inst Canc Res, Beijing 100036, Peoples R China
2.Peking Univ, Sch Oncol, Beijing Canc Hosp, Beijing 100036, Peoples R China

Recommended Citation:
Luo, DY,Zhang, BZ,Lv, LB,et al. Methylation of CpG islands of p16 associated with progression of primary gastric carcinomas[J]. LABORATORY INVESTIGATION,2006,86(6):591-598.
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