IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Ischemic preconditioning attenuates mitochondrial localization of PTEN induced by ischemia-reperfusion
Zu, Lingyun1,2; Zheng, Xiaoxu1; Wang, Bing3; Parajuli, Nirmal1; Steenbergen, Charles4; Becker, Lewis C.1; Cai, Zheqing P.1
关键词Bax Reperfusion Injury Apoptosis Phosphatase And Tensin Homologs Deleted On Chromosome 10
刊名AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
2011-06-01
DOI10.1152/ajpheart.01138.2010
300期:6页:H2177-H2186
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems ; Physiology ; Peripheral Vascular Disease
研究领域[WOS]Cardiovascular System & Cardiology ; Physiology
关键词[WOS]SIGNAL-REGULATED KINASE ; PERMEABILITY TRANSITION ; INTACT HEARTS ; NITRIC-OXIDE ; ISCHEMIA/REPERFUSION INJURY ; INDUCED CARDIOPROTECTION ; DIFFERENTIAL ACTIVATION ; PROSURVIVAL KINASES ; OXIDATIVE STRESS ; PROTEIN-KINASES
英文摘要

Zu L, Zheng X, Wang B, Parajuli N, Steenbergen C, Becker LC, Cai ZP. Ischemic preconditioning attenuates mitochondrial localization of PTEN induced by ischemia-reperfusion. Am J Physiol Heart Circ Physiol 300: H2177-H2186, 2011. First published March 18, 2011; doi: 10.1152/ajpheart.01138.2010.-Although the induction of myocyte apoptosis by ischemia-reperfusion (I/R) is attenuated by ischemic preconditioning (IPC), the underlying mechanism is not fully understood. Phosphatase and tensin homologs deleted on chromosome 10 (PTEN) promotes apoptosis through Akt-dependent and -independent mechanisms. We tested the hypothesis that IPC attenuates the mitochondrial localization of PTEN in the myocardium induced by I/R. Isolated hearts from wild-type mice were exposed to IPC or normal perfusion followed by 30 min of ischemia and reperfusion. IPC attenuated myocardial infarct size and apoptosis after I/R. Heart fractionation showed that mitochondrial PTEN and Bax protein levels and the physical association between them were increased by 30 min of I/R and that IPC attenuated all of these effects of I/R. Muscle-specific PTEN knockout decreased mitochondrial Bax protein levels in the reperfused myocardium and increased cell survival. To determine whether PTEN relocalization to mitochondria was influenced by I/R-induced production of ROS, hearts were perfused with N-acetylcysteine (NAC) to scavenge ROS or H(2)O(2) to mimic I/R-induced ROS. Mitochondrial PTEN protein levels were decreased by NAC and increased by H(2)O(2). PTEN protein overexpression was generated in mouse hearts by adenoviral gene transfer. PTEN overexpression increased mitochondrial PTEN and Bax protein levels and ROS production, whereas muscle-specific PTEN knockout produced the opposite effects. In conclusion, myocardial I/R causes PTEN localization to the mitochondria, related to the generation of ROS; IPC attenuates the mitochondrial localization of PTEN after I/R, potentially inhibiting the translocation of Bax to the mitochondria and resulting in improved cell viability.

语种英语
WOS记录号WOS:000291209300024
项目编号HL-65608 ; HL-88071
资助机构National Heart, Lung, and Blood Institute
引用统计
被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55367
专题北京大学第三临床医学院_心血管内科
作者单位1.Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD USA
2.Johns Hopkins Univ, Sch Med, Dept Med, Div Cardiol, Baltimore, MD 21205 USA
3.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100871, Peoples R China
4.Johns Hopkins Univ, Sch Med, Dept Anesthesiol, Baltimore, MD USA
推荐引用方式
GB/T 7714
Zu, Lingyun,Zheng, Xiaoxu,Wang, Bing,et al. Ischemic preconditioning attenuates mitochondrial localization of PTEN induced by ischemia-reperfusion[J]. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY,2011,300(6):H2177-H2186.
APA Zu, Lingyun.,Zheng, Xiaoxu.,Wang, Bing.,Parajuli, Nirmal.,Steenbergen, Charles.,...&Cai, Zheqing P..(2011).Ischemic preconditioning attenuates mitochondrial localization of PTEN induced by ischemia-reperfusion.AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY,300(6),H2177-H2186.
MLA Zu, Lingyun,et al."Ischemic preconditioning attenuates mitochondrial localization of PTEN induced by ischemia-reperfusion".AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY 300.6(2011):H2177-H2186.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zu, Lingyun]的文章
[Zheng, Xiaoxu]的文章
[Wang, Bing]的文章
百度学术
百度学术中相似的文章
[Zu, Lingyun]的文章
[Zheng, Xiaoxu]的文章
[Wang, Bing]的文章
必应学术
必应学术中相似的文章
[Zu, Lingyun]的文章
[Zheng, Xiaoxu]的文章
[Wang, Bing]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。