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学科主题: 临床医学
题名:
Changes in amount of ADM mRNA and RAMP2 mRNA in calcified vascular smooth muscle cells
作者: Qi, YF; Wang, SH; Zhang, BH; Bu, DF; Shu, TC; Du, JB
关键词: vascular smooth muscle cells (VSMCs) ; adrenomedullin (ADM) ; receptor activity modifying protein 2 (RAMP2) ; mRNA
刊名: PEPTIDES
发表日期: 2003-02-01
DOI: 10.1016/S0196-9781(03)00036-6
卷: 24, 期:2, 页:287-294
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: ACTIVITY-MODIFYING PROTEINS ; ARTERIAL CALCIFICATION ; ADRENOMEDULLIN GENE ; COMPETITIVE PCR ; IN-VITRO ; PEPTIDE ; RECEPTOR ; RATS ; ASSOCIATION ; OSTEOPONTIN
英文摘要:

This work was aimed to explore the changes and significance of adrenomedullin (ADM) mRNA and receptor activity modifying protein 2 (RAMP2) mRNA in calcified vascular smooth muscle cells (VSMCs). Calcification of cultured rat VSMCs was produced by incubation with beta-glycerophosphate. Content of ADM released by VSMCs was measured by radioimmunoassay (RIA). The amount of ADM mRNA and RAMP2 mRNA was determined by competitive quantitative RT-PCR. The intracellular calcium content, alkaline phosphatases activity and cellular Ca-45(2+)-uptake were determined. The results showed that the content of calcium, Ca-45(2+)-uptake and alkaline phosphatases activity in calcified VSMCs were increased by 118%, 174% and seven-fold (all P < 0.01), respectively, compared with control VSMCs. Content of ADM in medium was increased by 99% (P < 0.01). Furthermore, it was found that the amount of ADM mRNA and RAMP2 mRNA in calcified cells was elevated by 78 and 56% (all P < 0.05), respectively, compared with control. The elevated levels of RAMP2 mRNA were in positive correlation with ADM mRNA (r = 0.76, P < 0.05) in calcified VSMCs. In conclusion, calcified VSMCs generated an increased amount of ADM, and up-regulated gene expressions of ADM and RAMP2. (C) 2003 Elsevier Science Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000182338000015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55378
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Inst Cardiovasc Dis, Beijing 100034, Peoples R China
2.Peking Univ, Ctr Hlth Sci, Dept Physiol, Beijing 100083, Peoples R China
3.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China

Recommended Citation:
Qi, YF,Wang, SH,Zhang, BH,et al. Changes in amount of ADM mRNA and RAMP2 mRNA in calcified vascular smooth muscle cells[J]. PEPTIDES,2003,24(2):287-294.
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