北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 细胞生物学系  > 期刊论文
学科主题: 基础医学
题名:
Long-term Cultured Human Neural Stem Cells Undergo Spontaneous Transformation to Tumor-Initiating Cells
作者: Wu, Weihua1,2; He, Qihua2,3; Li, Xiaoxia2,4; Zhang, Xiaoyan1,2; Lu, Aili1,2; Ge, Ruimin1,2; Zhen, HongYing1,2; Chang, Alfred E.; Li, Qiao; Shen, Li1,2
关键词: tumorigenesis ; malignant transformation ; epidermal growth factor receptor (EGFR) ; xenograft
刊名: INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
发表日期: 2011
DOI: 10.7150/ijbs.7.892
卷: 7, 期:6, 页:892-901
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
英文摘要:

In this report, we describe the spontaneous malignant transformation of long-term cultured human fetal striatum neural stem cells (hsNSCs, passage 17). After subcutaneous transplantation of long-term cultured hsNSCs into immunodeficient nude mice, 2 out of 15 mice formed xenografts which expressed neuroendocrine tumor markers CgA and NSE. T1 cells, a cell line that we derived from one of the two subcutaneous xenografts, have undergone continuous expansion in vitro. These T1 cells showed stem cell-like features and expressed neural stem cell markers nestin and CD133. The T1 cells were involved in abnormal karyotype, genomic instability and fast proliferation. Importantly, after long-term in vitro culture, the T1 cells did not result in subcutaneous xenografts, but induced intracranial tumor formation, indicating that they adjusted themselves to the intracranial microenvironment. We further found that the T1 cells exhibited an overexpressed level of EGFR, and the CD133 positive T1 cells showed a truncation mutation in the exons 2-7 of the EGFR (EGFRvIII) gene. These results suggest that continuous expansion of neural stem cells in culture may lead to malignant spontaneous transformation. This phenomenon may be functionally related to EGFR by EGFRvIII gene mutation.

语种: 英语
所属项目编号: 30471638 ; 7062041 ; 2011CB510200 ; 2011CB707700
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Foundation of National Science and Technology Major Project Focusing on Drug Innovation from The Ministry of Science and Technology of the People&prime ; s Republic of China ; National Basic Research Program of China 973 Project ; Gillson Longenbaugh Foundation
WOS记录号: WOS:000208687900006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55386
Appears in Collections:基础医学院_细胞生物学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Stem Cell Res Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100191, Peoples R China
3.Peking Univ, Med & Hlth Anal Ctr, Beijing 100191, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100191, Peoples R China
5.Univ Michigan, Med Ctr, Dept Surg, Ann Arbor, MI 48109 USA

Recommended Citation:
Wu, Weihua,He, Qihua,Li, Xiaoxia,et al. Long-term Cultured Human Neural Stem Cells Undergo Spontaneous Transformation to Tumor-Initiating Cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2011,7(6):892-901.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wu, Weihua]'s Articles
[He, Qihua]'s Articles
[Li, Xiaoxia]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wu, Weihua]‘s Articles
[He, Qihua]‘s Articles
[Li, Xiaoxia]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace