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学科主题基础医学
Long-term Cultured Human Neural Stem Cells Undergo Spontaneous Transformation to Tumor-Initiating Cells
Wu, Weihua1,2; He, Qihua2,3; Li, Xiaoxia2,4; Zhang, Xiaoyan1,2; Lu, Aili1,2; Ge, Ruimin1,2; Zhen, HongYing1,2; Chang, Alfred E.; Li, Qiao; Shen, Li1,2
关键词Tumorigenesis Malignant Transformation Epidermal Growth Factor Receptor (Egfr) Xenograft
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
2011
DOI10.7150/ijbs.7.892
7期:6页:892-901
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
英文摘要

In this report, we describe the spontaneous malignant transformation of long-term cultured human fetal striatum neural stem cells (hsNSCs, passage 17). After subcutaneous transplantation of long-term cultured hsNSCs into immunodeficient nude mice, 2 out of 15 mice formed xenografts which expressed neuroendocrine tumor markers CgA and NSE. T1 cells, a cell line that we derived from one of the two subcutaneous xenografts, have undergone continuous expansion in vitro. These T1 cells showed stem cell-like features and expressed neural stem cell markers nestin and CD133. The T1 cells were involved in abnormal karyotype, genomic instability and fast proliferation. Importantly, after long-term in vitro culture, the T1 cells did not result in subcutaneous xenografts, but induced intracranial tumor formation, indicating that they adjusted themselves to the intracranial microenvironment. We further found that the T1 cells exhibited an overexpressed level of EGFR, and the CD133 positive T1 cells showed a truncation mutation in the exons 2-7 of the EGFR (EGFRvIII) gene. These results suggest that continuous expansion of neural stem cells in culture may lead to malignant spontaneous transformation. This phenomenon may be functionally related to EGFR by EGFRvIII gene mutation.

语种英语
WOS记录号WOS:000208687900006
项目编号30471638 ; 7062041 ; 2011CB510200 ; 2011CB707700
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Foundation of National Science and Technology Major Project Focusing on Drug Innovation from The Ministry of Science and Technology of the People&prime ; s Republic of China ; National Basic Research Program of China 973 Project ; Gillson Longenbaugh Foundation
引用统计
被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55386
专题北京大学基础医学院_细胞生物学系
北京大学医学部管理机构_医学部
北京大学基础医学院
作者单位1.Peking Univ, Stem Cell Res Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100191, Peoples R China
3.Peking Univ, Med & Hlth Anal Ctr, Beijing 100191, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100191, Peoples R China
5.Univ Michigan, Med Ctr, Dept Surg, Ann Arbor, MI 48109 USA
推荐引用方式
GB/T 7714
Wu, Weihua,He, Qihua,Li, Xiaoxia,et al. Long-term Cultured Human Neural Stem Cells Undergo Spontaneous Transformation to Tumor-Initiating Cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2011,7(6):892-901.
APA Wu, Weihua.,He, Qihua.,Li, Xiaoxia.,Zhang, Xiaoyan.,Lu, Aili.,...&Shen, Li.(2011).Long-term Cultured Human Neural Stem Cells Undergo Spontaneous Transformation to Tumor-Initiating Cells.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,7(6),892-901.
MLA Wu, Weihua,et al."Long-term Cultured Human Neural Stem Cells Undergo Spontaneous Transformation to Tumor-Initiating Cells".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 7.6(2011):892-901.
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