北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 北京大学人类疾病基因研究中心  > 期刊论文
学科主题: 基础医学
题名:
Programmed cell death protein 5 (PDCD5) is phosphorylated by CK2 in vitro and in 293T cells
作者: Salvi, Mauro1; Xu, Dong2; Chen, Yingyu2; Cabrelle, Anna3; Sarno, Stefania1,3; Pinna, Lorenzo A.1,3
关键词: PDCD5 ; Protein kinase CK2 ; Phosphorylation
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2009-09-25
DOI: 10.1016/j.bbrc.2009.07.067
卷: 387, 期:3, 页:606-610
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: KINASE CK2 ; APOPTOSIS ; TFAR19
英文摘要:

CK2 is a multifunctional kinase, involved in cell growth, apoptosis, DNA integrity preservation, viral infection, and many other biological processes. Based on an analysis of phosphopeptides database derived from phosphoproteomic Studies we previously identified a list of potential new CK2 substrates, including, among others, Programmed Cell Death 5 (PDCD5), a protein involved in cell death and down-regulated in different forms Of human tumors. Here we provide experimental evidence that PDCD5 is indeed a bona fide Substrate of CK2. PDCD5 is phosphorylated in vitro by both CK2 alpha. Subunit and by the CK2 holoenzyme at a residue, S 118, which is found phosphorylated in vivo. We also show that PDCD5 is phosphorylated by CK2 in 293T cells. Transfection of the non-phosphorylatable mutant (S118A) impairs the PDCD5 acceleration of either doxorubimicin or UV-induced apoptosis in U2OS cells. Our results Suggest a functional link between the CK2 phosphorylation and the apoptotic potential of PDCD5. (C) 2009 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30871263 ; 503467 ; FFC 4/2007
项目资助者: University of Padova, Progetto di Ateneo ; National Natural Science Foundation of China ; European Commission ; Italian Cystic Fibrosis Research Foundation ; Banca Popolare di Verona e Novara ; Fondazione Giorgio Zanotto
WOS记录号: WOS:000269137800036
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55389
Appears in Collections:基础医学院_北京大学人类疾病基因研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Programmed cell death protein 5 (PDCD5) is phosphorylated by CK2 in vitro and in 293T cells.pdf(571KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Univ Padua, Dept Biol Chem, I-35131 Padua, Italy
2.Venetian Inst Mol Med, I-35129 Padua, Italy
3.Peking Univ, Hlth Sci Ctr, Ctr Human Dis Genom, Beijing 100191, Peoples R China

Recommended Citation:
Salvi, Mauro,Xu, Dong,Chen, Yingyu,et al. Programmed cell death protein 5 (PDCD5) is phosphorylated by CK2 in vitro and in 293T cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2009,387(3):606-610.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Salvi, Mauro]'s Articles
[Xu, Dong]'s Articles
[Chen, Yingyu]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Salvi, Mauro]‘s Articles
[Xu, Dong]‘s Articles
[Chen, Yingyu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace