学科主题基础医学
Programmed cell death protein 5 (PDCD5) is phosphorylated by CK2 in vitro and in 293T cells
Salvi, Mauro1; Xu, Dong2; Chen, Yingyu2; Cabrelle, Anna3; Sarno, Stefania1,3; Pinna, Lorenzo A.1,3
关键词PDCD5 Protein kinase CK2 Phosphorylation
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2009-09-25
DOI10.1016/j.bbrc.2009.07.067
387期:3页:606-610
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]KINASE CK2 ; APOPTOSIS ; TFAR19
英文摘要

CK2 is a multifunctional kinase, involved in cell growth, apoptosis, DNA integrity preservation, viral infection, and many other biological processes. Based on an analysis of phosphopeptides database derived from phosphoproteomic Studies we previously identified a list of potential new CK2 substrates, including, among others, Programmed Cell Death 5 (PDCD5), a protein involved in cell death and down-regulated in different forms Of human tumors. Here we provide experimental evidence that PDCD5 is indeed a bona fide Substrate of CK2. PDCD5 is phosphorylated in vitro by both CK2 alpha. Subunit and by the CK2 holoenzyme at a residue, S 118, which is found phosphorylated in vivo. We also show that PDCD5 is phosphorylated by CK2 in 293T cells. Transfection of the non-phosphorylatable mutant (S118A) impairs the PDCD5 acceleration of either doxorubimicin or UV-induced apoptosis in U2OS cells. Our results Suggest a functional link between the CK2 phosphorylation and the apoptotic potential of PDCD5. (C) 2009 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000269137800036
项目编号30871263 ; 503467 ; FFC 4/2007
资助机构University of Padova, Progetto di Ateneo ; National Natural Science Foundation of China ; European Commission ; Italian Cystic Fibrosis Research Foundation ; Banca Popolare di Verona e Novara ; Fondazione Giorgio Zanotto
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55389
专题北京大学基础医学院_北京大学人类疾病基因研究中心
作者单位1.Univ Padua, Dept Biol Chem, I-35131 Padua, Italy
2.Venetian Inst Mol Med, I-35129 Padua, Italy
3.Peking Univ, Hlth Sci Ctr, Ctr Human Dis Genom, Beijing 100191, Peoples R China
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GB/T 7714
Salvi, Mauro,Xu, Dong,Chen, Yingyu,et al. Programmed cell death protein 5 (PDCD5) is phosphorylated by CK2 in vitro and in 293T cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2009,387(3):606-610.
APA Salvi, Mauro,Xu, Dong,Chen, Yingyu,Cabrelle, Anna,Sarno, Stefania,&Pinna, Lorenzo A..(2009).Programmed cell death protein 5 (PDCD5) is phosphorylated by CK2 in vitro and in 293T cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,387(3),606-610.
MLA Salvi, Mauro,et al."Programmed cell death protein 5 (PDCD5) is phosphorylated by CK2 in vitro and in 293T cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 387.3(2009):606-610.
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