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Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.1475c from caries pathogen Streptoeoeeus mutans
Zhou, YF; Mi, W; Li, LF; Zhang, XY; Liang, YH; Su, XD; Wei, SC
关键词Streptococcus Mutans Dental Caries Smu.1475c Protein Crystallography
刊名BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
2006-02-01
DOI10.1016/j.bbapap.2005.11.022
1764期:2页:324-326
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]CARIOGENIC DENTAL PATHOGEN ; STREPTOCOCCUS-MUTANS ; SEQUENCE ; DNA
英文摘要

The gene smu.1475c encodes a putative protein of 211 residues in Streptococcus mutans, a primary pathogen for human dental caries. In this work, smu. 1475c was cloned into pET28a and expressed in good amount from the E. coli strain BL21 (DE3). Smu. 1475c protein was purified to homogeneity in a two-step procedure of Ni2+ chelating and size exclusion chromatography. Crystals were obtained by hanging-drop vapor-diffusion method and diffracted to 2.7 angstrom resolution. The crystal belongs to orthorhombic space group P2(1)2(1)2(1) with cell dimension of a= 68.3 angstrom, b= 105.9 angstrom, c= 136.2 angstrom. The asymmetric unit is expected to contain four molecules with solvent content of 49.4%. (c) 2005 Elsevier B.V All rights reserved.

语种英语
WOS记录号WOS:000236101500020
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55392
专题北京大学口腔医学院
作者单位1.Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China
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GB/T 7714
Zhou, YF,Mi, W,Li, LF,et al. Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.1475c from caries pathogen Streptoeoeeus mutans[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2006,1764(2):324-326.
APA Zhou, YF.,Mi, W.,Li, LF.,Zhang, XY.,Liang, YH.,...&Wei, SC.(2006).Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.1475c from caries pathogen Streptoeoeeus mutans.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1764(2),324-326.
MLA Zhou, YF,et al."Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.1475c from caries pathogen Streptoeoeeus mutans".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1764.2(2006):324-326.
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