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学科主题: 口腔医学
题名:
Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.1475c from caries pathogen Streptoeoeeus mutans
作者: Zhou, YF; Mi, W; Li, LF; Zhang, XY; Liang, YH; Su, XD; Wei, SC
关键词: streptococcus mutans ; dental caries ; Smu.1475c ; protein crystallography
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
发表日期: 2006-02-01
DOI: 10.1016/j.bbapap.2005.11.022
卷: 1764, 期:2, 页:324-326
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: CARIOGENIC DENTAL PATHOGEN ; STREPTOCOCCUS-MUTANS ; SEQUENCE ; DNA
英文摘要:

The gene smu.1475c encodes a putative protein of 211 residues in Streptococcus mutans, a primary pathogen for human dental caries. In this work, smu. 1475c was cloned into pET28a and expressed in good amount from the E. coli strain BL21 (DE3). Smu. 1475c protein was purified to homogeneity in a two-step procedure of Ni2+ chelating and size exclusion chromatography. Crystals were obtained by hanging-drop vapor-diffusion method and diffracted to 2.7 angstrom resolution. The crystal belongs to orthorhombic space group P2(1)2(1)2(1) with cell dimension of a= 68.3 angstrom, b= 105.9 angstrom, c= 136.2 angstrom. The asymmetric unit is expected to contain four molecules with solvent content of 49.4%. (c) 2005 Elsevier B.V All rights reserved.

语种: 英语
WOS记录号: WOS:000236101500020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55392
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China
2.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China

Recommended Citation:
Zhou, YF,Mi, W,Li, LF,et al. Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.1475c from caries pathogen Streptoeoeeus mutans[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2006,1764(2):324-326.
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