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IR@PKUHSC  > 北京大学第二临床医学院  > 消化内科  > 期刊论文
学科主题: 临床医学
题名:
Intestinal immune barrier integrity in rats with nonalcoholic hepatic steatosis and steatohepatitis
作者: Su Lin1,3,4; Wang Jian-hua; Cong Xu2; Wang Li-hong1; Liu Feng2; Xie Xing-wang2; Zhang Heng-hui2; Wang Jiang-hua2,3,4; Fei Ran2; Liu Yu-lan1
关键词: non-alcoholic fatty liver disease ; intestinal immune barrier ; endotoxins ; CD4/CD8 ; immunoglobulin A, secretory
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2012-01-20
DOI: 10.3760/cma.j.issn.0366-6999.2012.02.026
卷: 125, 期:2, 页:306-311
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: FATTY LIVER-DISEASE ; SINUSOIDAL-ENDOTHELIAL-CELLS ; OBESE MICE ; GUT ; PERMEABILITY ; INFLAMMATION ; ENDOTOXIN ; DIET ; PATHOGENESIS ; TOLERANCE
英文摘要:

Background Nonalcoholic fatty liver disease (NAFLD) has emerged as the major cause of chronic liver injury. Intestinal barrier plays an important role in the pathogenis of NAFLD. The aim of this article was to assess intestinal immune barrier function during the development of NAFLD.

Methods Totally 60 male Sprague-Dawley (SD) rats were divided into 2 groups: normal diet (ND) group and high-fat diet (HFD) group. NAFLD rat model was established in the HFD rat group. Portal blood endotoxin level was assessed by limulus test. The percentage of CD4+ cells and CD8+ cells in peripheral blood mononuclear cells (PBMC) and lymphocytes in Peyer′s patches (PP) were analysed by flow cytometry. Intestinal secretory immunoglobulin A (SIgA) level was evaluated by enzyme-linked immunosorbent assay. Paired Student′s t test was used for the statistic analysis.

Results HFD rats presented with simple steatosis at the 4th and 8th week and progressed to nonalcoholic steatohepatitis at the 12th week. Elevated lipopolysaccharides (LPS) level in HFD rats was observed at the 8th week ((1.54+/-0.30) times of ND group, P <0.01). CD4/CD8 ratios in PBMC and PP of HFD rats were increased at the 4th week ((1.50+/-0.47) and (1.63+/-0.34) times of ND group, P <0.05) and decreased at the 8th week ((0.50+/-0.16) and (0.61+/-0.26) times of ND group, P <0.05). At the 12th week, CD4/CD8 ratio ((1.47+/-0.46) times, P <0.05) in PP increased to levels observed in the 4th week. Intestinal SIgA expression of HFD rats was remarkably up-regulated at 12th week ((2.70+/-1.65) times, P <0.05).

Conclusion Liver-gut axis in rats with NAFLD may mediate and improve intestinal immune function by increased CD4/CD8 ratio in PP and increased production of SIgA. Chin Med J 2012;125(2):306-311

语种: 英语
WOS记录号: WOS:000300749500027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55401
Appears in Collections:北京大学第二临床医学院_消化内科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Gastroenterol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
3.Univ Sydney, Westmead Millenium Inst, Storr Liver Unit, Sydney, NSW 2145, Australia
4.Westmead Hosp, Sydney, NSW 2145, Australia

Recommended Citation:
Su Lin,Wang Jian-hua,Cong Xu,et al. Intestinal immune barrier integrity in rats with nonalcoholic hepatic steatosis and steatohepatitis[J]. CHINESE MEDICAL JOURNAL,2012,125(2):306-311.
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