|Intestinal immune barrier integrity in rats with nonalcoholic hepatic steatosis and steatohepatitis|
|Su Lin1,3,4; Wang Jian-hua; Cong Xu2; Wang Li-hong1; Liu Feng2; Xie Xing-wang2; Zhang Heng-hui2; Wang Jiang-hua2,3,4; Fei Ran2; Liu Yu-lan1|
|关键词||Non-alcoholic Fatty Liver Disease Intestinal Immune Barrier Endotoxins Cd4/cd8 Immunoglobulin a Secretory|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||FATTY LIVER-DISEASE ; SINUSOIDAL-ENDOTHELIAL-CELLS ; OBESE MICE ; GUT ; PERMEABILITY ; INFLAMMATION ; ENDOTOXIN ; DIET ; PATHOGENESIS ; TOLERANCE|
Background Nonalcoholic fatty liver disease (NAFLD) has emerged as the major cause of chronic liver injury. Intestinal barrier plays an important role in the pathogenis of NAFLD. The aim of this article was to assess intestinal immune barrier function during the development of NAFLD.
Methods Totally 60 male Sprague-Dawley (SD) rats were divided into 2 groups: normal diet (ND) group and high-fat diet (HFD) group. NAFLD rat model was established in the HFD rat group. Portal blood endotoxin level was assessed by limulus test. The percentage of CD4+ cells and CD8+ cells in peripheral blood mononuclear cells (PBMC) and lymphocytes in Peyer′s patches (PP) were analysed by flow cytometry. Intestinal secretory immunoglobulin A (SIgA) level was evaluated by enzyme-linked immunosorbent assay. Paired Student′s t test was used for the statistic analysis.
Results HFD rats presented with simple steatosis at the 4th and 8th week and progressed to nonalcoholic steatohepatitis at the 12th week. Elevated lipopolysaccharides (LPS) level in HFD rats was observed at the 8th week ((1.54+/-0.30) times of ND group, P <0.01). CD4/CD8 ratios in PBMC and PP of HFD rats were increased at the 4th week ((1.50+/-0.47) and (1.63+/-0.34) times of ND group, P <0.05) and decreased at the 8th week ((0.50+/-0.16) and (0.61+/-0.26) times of ND group, P <0.05). At the 12th week, CD4/CD8 ratio ((1.47+/-0.46) times, P <0.05) in PP increased to levels observed in the 4th week. Intestinal SIgA expression of HFD rats was remarkably up-regulated at 12th week ((2.70+/-1.65) times, P <0.05).
Conclusion Liver-gut axis in rats with NAFLD may mediate and improve intestinal immune function by increased CD4/CD8 ratio in PP and increased production of SIgA. Chin Med J 2012;125(2):306-311
|作者单位||1.Peking Univ, Peoples Hosp, Dept Gastroenterol, Beijing 100044, Peoples R China|
2.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
3.Univ Sydney, Westmead Millenium Inst, Storr Liver Unit, Sydney, NSW 2145, Australia
4.Westmead Hosp, Sydney, NSW 2145, Australia
|Su Lin,Wang Jian-hua,Cong Xu,et al. Intestinal immune barrier integrity in rats with nonalcoholic hepatic steatosis and steatohepatitis[J]. CHINESE MEDICAL JOURNAL,2012,125(2):306-311.|
|APA||Su Lin.,Wang Jian-hua.,Cong Xu.,Wang Li-hong.,Liu Feng.,...&Liu Yu-lan.(2012).Intestinal immune barrier integrity in rats with nonalcoholic hepatic steatosis and steatohepatitis.CHINESE MEDICAL JOURNAL,125(2),306-311.|
|MLA||Su Lin,et al."Intestinal immune barrier integrity in rats with nonalcoholic hepatic steatosis and steatohepatitis".CHINESE MEDICAL JOURNAL 125.2(2012):306-311.|