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学科主题: 药物依赖
题名:
Enhanced antidepressant-like effects of the macromolecule trefoil factor 3 by loading into negatively charged liposomes
作者: Qin, Jing1; Yang, Xu1,2,3; Mi, Jia4; Wang, Jianxin1; Hou, Jia1,2; Shen, Teng1; Li, Yongji2; Wang, Bin4; Li, Xuri; Zhu, Weili5
关键词: TFF3 ; drug delivery system ; monocytes ; brain-targeting drug delivery ; antidepressant ; blood-brain barrier
刊名: INTERNATIONAL JOURNAL OF NANOMEDICINE
发表日期: 2014
DOI: 10.2147/OPTH.S69335
卷: 9, 页:5247-5257
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
研究领域[WOS]: Science & Technology - Other Topics ; Pharmacology & Pharmacy
关键词[WOS]: BLOOD-BRAIN-BARRIER ; MONONUCLEAR-CELLS ; ENDOTHELIAL-CELLS ; THP-1 CELLS ; DEPRESSION ; MODEL ; DRUGS ; MACROPHAGES ; EXPRESSION ; MONOCYTES
英文摘要:

Immunocytes, mainly neutrophils and monocytes, exhibit an intrinsic homing property, enabling them to migrate to sites of injury and inflammation. They can thus act as Trojan horses carrying concealed drug cargoes while migrating across impermeable barriers to sites of disease, especially the blood-brain barrier (BBB). In this study, to target circulating phagocytic cells, we formulated negatively charged nanosize liposomes and loaded trefoil factor 3 (TFF3) into liposomes by the pH-gradient method. According to the optimized formulation (5:1.5 of lipid to cholesterol, 10: 1 of lipid to drug, 10 mg/mL of lipid concentration, and 10 mmol/L of phosphate-buffered saline), 44.47% entrapment efficiency was obtained for TFF3 liposomes with 129.6 nm particle size and -36.6 mV zeta potential. Compared with neutrally charged liposomes, the negatively charged liposomes showed a strong binding capacity with monocytes and were effectively carried by monocytes to cross the BBB in vitro. Furthermore, enhanced antidepressant-like effects were found in the tail-suspension and forced-swim tests in mice, as measured by decreased immobility time, as well as increased swimming time and reduced immobility in rats. These results suggested that negatively charged liposomes could improve the behavioral responses of TFF3, and our study opens up a new way for the development of effective therapies for brain disease by increasing the permeability of the BBB.

语种: 英语
所属项目编号: 81102386 ; 81371489 ; YETP0068 ; 2012ZX09103-301-049 ; 20110071120042 ; 2013CB932502
项目资助者: National Natural Science Foundation of China ; Beijing Higher Education Young Elite Teacher Project ; National Science and Technology Major Project ; Specialized Research Fund for the Doctoral Program of Higher Education ; School of Pharmacy, Fudan University ; Open Project Program of Key Lab of Smart Drug Delivery (Fudan University), Ministry of Education, PRC ; Program for Scientific Research Innovation team in Colleges and Universities of Shandong Province ; Taishan Scholars Construction Engineering ; National Key Basic Research Program of China (program 973)
WOS记录号: WOS:000344808500001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55406
Appears in Collections:中国药物依赖性研究所_期刊论文

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作者单位: 1.Fudan Univ, Sch Pharm, Dept Pharmaceut, Key Lab Smart Drug Delivery,Minist Educ, Shanghai 200433, Peoples R China
2.Heilongjiang Univ Chinese Med, Sch Pharm, Dept Pharmaceut, Harbin, Peoples R China
3.Fudan Univ, Peoples Hosp 5, Dept Pharm, Shanghai 200433, Peoples R China
4.Binzhou Med Univ, Yantai, Peoples R China
5.Peking Univ, Natl Inst Drug Dependence, Beijing 100191, Peoples R China

Recommended Citation:
Qin, Jing,Yang, Xu,Mi, Jia,et al. Enhanced antidepressant-like effects of the macromolecule trefoil factor 3 by loading into negatively charged liposomes[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2014,9:5247-5257.
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