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Adeno-associated virus-mediated bone morphogenetic protein-7 gene transfer induces C2C12 cell differentiation into osteoblast lineage cells
Yang, M; Ma, QJ; Dang, GT; Ma, KT; Chen, P; Zhou, CY
关键词C2c12 Adeno-associated Virus Bone Morphogenetic Protein-7 Gene Therapy
刊名ACTA PHARMACOLOGICA SINICA
2005-08-01
DOI10.1111/j.1745-7254.2005.00159.x
26期:8页:963-968
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]MARROW STROMAL CELLS ; FORMATION IN-VITRO ; PERIOSTEAL CELLS ; EXPRESSION ; THERAPY ; VIVO ; VECTORS ; PROMOTER ; ALPHA
英文摘要

Aim: To investigate the effects of bone morphogenetic protein-7 (BMP7)-expressing recombinant adeno-associated virus (AAV) vector on the differentiation of C2C12 cells. Methods: AAV-BMP7 was packaged by infecting the stable cell clone BHK-21 (integrated with recombinant AAV vector plasmid pSNAV-BMP7) with recombinant herpes simplex virus type 1, which expresses AAV-2 Rep and Cap and possesses AAV packaging functions. Following infection with AAV-BMP7 at multiplicities of infection of 1 x 10(5) vector genomes per cell and subsequent culture, C2C12 cells were assessed qualitatively for BMP7 production, alkaline phosphatase activity, osteocalcin production and Cbfal and MyoD expression. Results: C2C12 cells transduced with AAV-BMP7 could produce BMP7 protein until d 28. Alkaline phosphatase in the cultured C2C12 cell lysate was elevated. Secreted osteocalcin in the culture medium was detectable at d 12 and Cbfal mRNA expression level was upregulated, coinciding with downregulation of MyoD in a temporal manner. Conclusion: The present in vitro study demonstrated that AAV-BMP7 could infect and efficiently convert C2C12 cells from myoblasts into osteoblast lineage cells.

语种英语
WOS记录号WOS:000231255700009
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55408
专题基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Dept Orthoped, Beijing 100083, Peoples R China
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Yang, M,Ma, QJ,Dang, GT,et al. Adeno-associated virus-mediated bone morphogenetic protein-7 gene transfer induces C2C12 cell differentiation into osteoblast lineage cells[J]. ACTA PHARMACOLOGICA SINICA,2005,26(8):963-968.
APA Yang, M,Ma, QJ,Dang, GT,Ma, KT,Chen, P,&Zhou, CY.(2005).Adeno-associated virus-mediated bone morphogenetic protein-7 gene transfer induces C2C12 cell differentiation into osteoblast lineage cells.ACTA PHARMACOLOGICA SINICA,26(8),963-968.
MLA Yang, M,et al."Adeno-associated virus-mediated bone morphogenetic protein-7 gene transfer induces C2C12 cell differentiation into osteoblast lineage cells".ACTA PHARMACOLOGICA SINICA 26.8(2005):963-968.
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