IR@PKUHSC  > 北京大学深圳医院  > 皮肤性病科
学科主题临床医学
High-resolution melting analysis of ADAMTS18 methylation levels in gastric, colorectal and pancreatic cancers
Li, Zhi2,3; Zhang, Wei4; Shao, Yong1,5; Zhang, Chao1; Wu, Qi1; Yang, Hong5; Wan, Xiangbin3; Zhang, Jie5; Guan, Ming6; Wan, Jun1,7; Yu, Bo1,5
关键词Adamts18 Methylation Hrm Gastric Cancer Colorectal Cancer Pancreatic Cancer
刊名MEDICAL ONCOLOGY
2010-09-01
DOI10.1007/s12032-009-9323-8
27期:3页:998-1004
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]DNA METHYLATION ; EMERGING ROLES ; CELL-LINES ; PCR BIAS ; FAMILY ; GENE ; METALLOPROTEINASES ; NASOPHARYNGEAL ; IDENTIFICATION ; AGGRECANASE-1
英文摘要

A disintegrin and metalloprotease with trombo-spondin motifs (ADAMTS) is a family of proteins characterized by the presence of a metalloproteinase domain linked to a variety of specialized ancillary domains. ADAMTS18 is a putative tumor suppressive gene related to nasopharyngeal carcinoma. We used high-resolution melting (HRM) analysis to detect the methylation levels of ADAMTS18 gene in 100 gastric cancers, 100 colorectal cancers, 70 pancreatic cancers, and equal number of adjacent normal tissues. The frequency of ADAMTS18 methylation in all three types of cancers was significantly higher than that in normal tissues. Expression levels of ADAMTS18 were inversely correlated with methylation levels. No significant association was found between ADAMTS18 methylation status and TNM staging in the cancers. In summary, epigenetic regulation of ADAMTS18 was associated with carcinogenesis. The application of HRM analysis is a fast and high-throughput way to investigate the epigenetic status of ADAMTS18.

语种英语
WOS记录号WOS:000281522400068
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55420
Collection北京大学深圳医院_皮肤性病科
作者单位1.Henan Prov Tumor Hosp, Dept Gen Surg, Zhengzhou, Henan, Peoples R China
2.Shenzhen PKU HKUST Med Ctr, Biomed Res Inst, Shenzhen, Guangdong, Peoples R China
3.Zhengzhou Univ, Basic Med Coll, Dept Histol & Embryol, Zhengzhou, Henan, Peoples R China
4.Ji Nan Univ, HKUST JNU Joint Lab, Guangzhou, Guangdong, Peoples R China
5.Peking Univ, Shenzhen Hosp, Dept Dermatol, Shen Zhen, Guangdong, Peoples R China
6.Fudan Univ, Huashan Hosp, Cent Lab, Dept Lab Med, Shanghai 200433, Peoples R China
7.Hong Kong Univ Sci & Technol, Dept Biochem, Kowloon, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Li, Zhi,Zhang, Wei,Shao, Yong,et al. High-resolution melting analysis of ADAMTS18 methylation levels in gastric, colorectal and pancreatic cancers[J]. MEDICAL ONCOLOGY,2010,27(3):998-1004.
APA Li, Zhi.,Zhang, Wei.,Shao, Yong.,Zhang, Chao.,Wu, Qi.,...&Yu, Bo.(2010).High-resolution melting analysis of ADAMTS18 methylation levels in gastric, colorectal and pancreatic cancers.MEDICAL ONCOLOGY,27(3),998-1004.
MLA Li, Zhi,et al."High-resolution melting analysis of ADAMTS18 methylation levels in gastric, colorectal and pancreatic cancers".MEDICAL ONCOLOGY 27.3(2010):998-1004.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Li, Zhi]'s Articles
[Zhang, Wei]'s Articles
[Shao, Yong]'s Articles
百度学术
百度学术Similar articles in
[Li, Zhi]'s Articles
[Zhang, Wei]'s Articles
[Shao, Yong]'s Articles
必应学术
必应学术Similar articles in
[Li, Zhi]'s Articles
[Zhang, Wei]'s Articles
[Shao, Yong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.