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学科主题: 口腔医学
题名:
Decreased Osteogenesis in Stromal Cells from Radiolucent Zone of Human TMJ Ankylosis
作者: Xiao, E.1; Li, J. -M.1; Yan, Y. -B.4; An, J. -G.1; Duan, D. -H.2; Gan, Y. -H.3; Zhang, Y.1
关键词: bone non-union ; pathogenesis ; condyle fracture ; mesenchymal stem cells ; bone formation ; bone proliferation
刊名: JOURNAL OF DENTAL RESEARCH
发表日期: 2013-05-01
DOI: 10.1177/0022034513483471
卷: 92, 期:5, 页:450-455
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: MESENCHYMAL PROGENITOR CELLS ; FRACTURE NON-UNIONS ; TEMPOROMANDIBULAR-JOINT ; STEM-CELLS ; BONY ANKYLOSIS ; OSTEOBLAST DIFFERENTIATION ; GENE-EXPRESSION ; IN-VITRO ; TISSUE ; INDUCTION
英文摘要:

We previously hypothesized that the development of traumatic temporomandibular joint (TMJ) ankylosis was similar to that of hypertrophic non-union. Besides similarities in etiology, hypertrophic bone stumps, and long-term development, the radiolucent zone, frequently located in the ankylosed bone, is another common feature. In this study, we demonstrated that the radiolucent zone also contained multilineage potential cells (RZs, radiolucent-zone-related cells) as the non-union tissues. RZs were characterized and compared with mandibular bone marrow stem cells (BMSCs) by analysis of MSC-related markers, colony-forming-unit assays, multipotential differentiation assays, alkaline phosphatase (ALP) activity assays, and cell transplantation in vivo. Both cell types were positive for CD105, CD166, and Stro-1 expression, negative for CD34 and CD45 expression, and exhibited osteogenic, adipogenic, and chondrogenic differentiation potentials. However, compared with mandibular BMSCs, RZs showed lower colony-forming-unit abilities and proliferation rates. The mineralization and bone-forming ability of RZs was weaker than that of mandibular BMSCs, with Runx2 and ALP mRNA expression and ALP activity significantly lower in RZs. All these results suggest that RZs possess the properties of MSCs but lower proliferation and osteogenic differentiation capacity similar to that of stromal cells in hypertrophic non-union tissues.

语种: 英语
所属项目编号: 81170936
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000317715300010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55428
Appears in Collections:北京大学口腔医学院_综合治疗科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Gen Dent, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
4.Tianjin Stomatol Hosp, Dept Oral & Maxillofacial Surg, Tianjin, Peoples R China

Recommended Citation:
Xiao, E.,Li, J. -M.,Yan, Y. -B.,et al. Decreased Osteogenesis in Stromal Cells from Radiolucent Zone of Human TMJ Ankylosis[J]. JOURNAL OF DENTAL RESEARCH,2013,92(5):450-455.
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