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学科主题: 临床医学
题名:
Rolling circle amplification and multiplex allele-specific PCR for rapid detection of katG and inhA gene mutations in Mycobacterium tuberculosis
作者: Cai, Lin2; Kong, Fanrong1; Jelfs, Peter1; Gilbert, Gwendolyn L.1,3; Sintchenko, Vitali1,3
关键词: Mycobacterium tuberculosis ; Drug resistance ; Resistance mutations
刊名: INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
发表日期: 2009-12-01
DOI: 10.1016/j.ijmm.2009.05.006
卷: 299, 期:8, 页:574-581
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology ; Virology
研究领域[WOS]: Microbiology ; Virology
关键词[WOS]: ISONIAZID RESISTANCE ; MOLECULAR CHARACTERIZATION ; RIFAMPIN RESISTANCE ; RUSSIAN-FEDERATION ; DRUG-RESISTANCE ; ASSAY ; IDENTIFICATION ; TRANSMISSION ; STRAINS ; REGION
英文摘要:

The aim of the study was to compare a novel, rolling circle amplification (RCA) assay for detection of common isoniazid (INH) resistance mutations in Mycobacterium tuberculosis with a multiplex allele-specific PCR (MAS-PCR) and sequencing of katG and the fabG1-inhA promoter region. One or more mutations were identified by RCA, MAS-PCR, and sequencing in 21 (68%), 22 (71%), and 23 (74%), respectively, of 31 epidemiologically unrelated INH-resistant isolates, and in none of 8 INH-susceptible isolates. The RCA assay is a rapid, inexpensive, and practical screening method for INH resistance in M. tuberculosis in countries with high prevalence of INH resistance. (C) 2009 Elsevier GmbH. All rights reserved.

语种: 英语
WOS记录号: WOS:000272186100005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55431
Appears in Collections:北京大学第二临床医学院_皮科_期刊论文

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作者单位: 1.Westmead Hosp, Sydney W Area Hlth Serv, ICPMR, CIDM PH, Sydney, NSW 2145, Australia
2.Beijing Univ, Peoples Hosp, Dept Dermatol, Beijing 100044, Peoples R China
3.Univ Sydney, Western Clin Sch, Sydney, NSW 2006, Australia

Recommended Citation:
Cai, Lin,Kong, Fanrong,Jelfs, Peter,et al. Rolling circle amplification and multiplex allele-specific PCR for rapid detection of katG and inhA gene mutations in Mycobacterium tuberculosis[J]. INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY,2009,299(8):574-581.
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