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学科主题: 临床医学
题名:
Isothermally Sensitive Detection of Serum Circulating miRNAs for Lung Cancer Diagnosis
作者: Li, Ying1; Liang, Li2; Zhang, Chun-yang1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2013-12-03
DOI: 10.1021/ac403462f
卷: 85, 期:23, 页:11174-11179
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: ROLLING-CIRCLE AMPLIFICATION ; MICRORNAS ; BIOMARKERS ; ASSAY
英文摘要:

Tumor-derived miRNAs in serum are emerging as the new noninvasive biomarkers for the diagnosis of human cancers, especially at their early stage. An ideal method with high sensitivity, excellent selectivity, a simple procedure, and small amounts of starting materials is imperative for the detection of clinic circulating miRNAs. Here, we develop a new method for isothermally sensitive detection of serum miRNAs using hairpin probe-based rolling circle amplification (HP-RCA). This method exhibits ultrahigh sensitivity toward lung cancer-related miR-486-5p with a detection limit of as low as 10 fM and a large dynamic range of 6 orders of magnitude, and it can even discriminate miR-486-5p from both miRNAs with high sequence homology and its precursors (pre-miRNAs). More importantly, this method can directly and accurately distinguish the expression of serum miR-486-5p among six nonsmall-cell lung carcinoma (NSCLC) patients and six healthy persons, holding a great potential for further applications in the clinical diagnosis of lung cancers.

语种: 英语
所属项目编号: 2011CB933600 ; 2010CB732600 ; 21325523 ; 21075129 ; 201002009 ; 2013-BYSY-CAS-007 ; JCYJ20130401170306879 ; (2012) 433 ; (2012) 433
项目资助者: National Basic Research Program 973 ; National Natural Science Foundation of China ; Chinese Academy of Science ; State Ministry of Health of China ; Peking University Third Hospital of China ; Guangdong Innovation Research Team Fund for Low-cost Healthcare Technologies ; Natural Science Foundation of Shenzhen City ; Fund for Guangdong Key Laboratory of Nanomedicine ; Fund for Shenzhen Engineering Laboratory of Single-molecule Detection and Instrument Development
WOS记录号: WOS:000327999800003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55451
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Chinese Acad Sci, Shenzhen Inst Adv Technol, Single Mol Detect & Imaging Lab, Shenzhen, Guangdong 518055, Peoples R China
2.Peking Univ, Hosp 3, Dept Tumor Chemotherapy & Radiat Sickness, Beijing 100191, Peoples R China

Recommended Citation:
Li, Ying,Liang, Li,Zhang, Chun-yang. Isothermally Sensitive Detection of Serum Circulating miRNAs for Lung Cancer Diagnosis[J]. ANALYTICAL CHEMISTRY,2013,85(23):11174-11179.
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