IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Allitridi induces apoptosis by affecting Bcl-2 expression and caspase-3 activity in human gastric cancer cells
Lan, H; Lu, YY
关键词Human Gastric Cancer Cell Line Apoptosis Diallyl Trisulfide Bcl-2 Cpp32 Protein
刊名ACTA PHARMACOLOGICA SINICA
2004-02-01
25期:2页:219-225
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]ALLYL SULFUR-COMPOUNDS ; HIGH-EPIDEMIC AREA ; STOMACH-CANCER ; DIALLYL-DISULFIDE ; ANTITUMOR-ACTIVITY ; ALLIUM VEGETABLES ; JIANGSU PROVINCE ; GARLIC POWDER ; HL-60 CELLS ; DNA-ADDUCTS
英文摘要

AIM: To investigate the mechanism of allitridi-induced apoptosis in human gastric cancer cell line BGC823. METHODS: Growth inhibition by allitridi was analyzed using cell growth curve and MTT assay. Apoptotic cells were detected using staining with Hoechst 33342, and confirmed by flow cytometric analysis and DNA fragmentation analysis. The protein expression affected by allitridi was determined using Western blot. The activity of caspase-3 was measured using a fluorescence assay. RESULTS: Allitridi induced apoptosis, and then inhibited cells proliferation in human gastric cancer cell line BGC823. The protein level of Bcl-2 was decreased dramatically, while Bax and p53 were not significantly affected by allitridi. The expression and activity of caspase-3 started to increase after allitridi treatment for 72 h. CONCLUSION: Allitridi induced apoptosis through down-regulation of Bcl-2, and increased caspase-3 expression and its activity.

语种中文
WOS记录号WOS:000188877300016
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55466
专题北京大学临床肿瘤学院
作者单位Peking Univ, Beijing Inst Canc Res, Sch Oncol, Beijing 100034, Peoples R China
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GB/T 7714
Lan, H,Lu, YY. Allitridi induces apoptosis by affecting Bcl-2 expression and caspase-3 activity in human gastric cancer cells[J]. ACTA PHARMACOLOGICA SINICA,2004,25(2):219-225.
APA Lan, H,&Lu, YY.(2004).Allitridi induces apoptosis by affecting Bcl-2 expression and caspase-3 activity in human gastric cancer cells.ACTA PHARMACOLOGICA SINICA,25(2),219-225.
MLA Lan, H,et al."Allitridi induces apoptosis by affecting Bcl-2 expression and caspase-3 activity in human gastric cancer cells".ACTA PHARMACOLOGICA SINICA 25.2(2004):219-225.
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