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学科主题: 临床医学
题名:
Three step derivation of cartilage like tissue from human embryonic stem cells by 2D-3D sequential culture in vitro and further implantation in vivo on alginate/PLGA scaffolds
作者: Bai, Hai Yan1,2; Chen, Gui An1,2; Mao, Gen Hong1,2; Song, Tian Ran1,2; Wang, Yan Xia1,2
关键词: human embryonic stem cell ; differentiation ; chondrocyte ; cartilage like tissue ; 2D-3D sequential cultivation ; alginate/PLGA compounds
刊名: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
发表日期: 2010-08-01
DOI: 10.1002/jbm.a.32732
卷: 94A, 期:2, 页:539-546
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: CHONDROGENIC DIFFERENTIATION ; PROGENITOR CELLS
英文摘要:

In this study a three step culture system, 2D-3D sequential culture in vitro and further implantation in vivo was developed to induce human embryonic stem cells (hESCs) into cartilage like tissues. Five-day-old embryoid bodies were plated for chondrogenic induction for 27 days (step1), then the cells were suspended in alginate and seeded onto polylactic-co-glycolic acid (PLGA) scaffolds for 3D cultivation for 7 days (step 2) and the cells/alginate/PLGA complexes were further transplanted into nude mice for 8 weeks (step 3). At same time, some of complexes were cultured in vitro up to 8 weeks. At the end of step 1, cells exhibited fibroblast-like morphology and expressed chondrocyte-specific markers, Sox 9 and collagen II. During the following 8 weeks of 3D cultivation in vitro, cells displayed spherical morphology, decreased immunoreactivity to Sox-9 and increased one to collagen II, demonstrated further differentiation to mature chondrocyte. In implanted grafts, not only cells appeared typical chondrocytes shape and markers but also cartilage like tissues were formed. These results indicate that 2D-3D sequential culture in vitro is an efficient protocol to induce hESCs differentiates into chondrocytes, while the three step culture system may be an appropriate procedure to derive cartilage like tissues from hESCs. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 94A: 539-546, 2010

语种: 英语
WOS记录号: WOS:000279482600024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55486
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Reprod Med Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Stem Cell Res Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Bai, Hai Yan,Chen, Gui An,Mao, Gen Hong,et al. Three step derivation of cartilage like tissue from human embryonic stem cells by 2D-3D sequential culture in vitro and further implantation in vivo on alginate/PLGA scaffolds[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2010,94A(2):539-546.
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