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Three step derivation of cartilage like tissue from human embryonic stem cells by 2D-3D sequential culture in vitro and further implantation in vivo on alginate/PLGA scaffolds
Bai, Hai Yan1,2; Chen, Gui An1,2; Mao, Gen Hong1,2; Song, Tian Ran1,2; Wang, Yan Xia1,2
关键词Human Embryonic Stem Cell Differentiation Chondrocyte Cartilage Like Tissue 2d-3d Sequential Cultivation Alginate/plga Compounds
刊名JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
2010-08-01
DOI10.1002/jbm.a.32732
94A期:2页:539-546
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]CHONDROGENIC DIFFERENTIATION ; PROGENITOR CELLS
英文摘要

In this study a three step culture system, 2D-3D sequential culture in vitro and further implantation in vivo was developed to induce human embryonic stem cells (hESCs) into cartilage like tissues. Five-day-old embryoid bodies were plated for chondrogenic induction for 27 days (step1), then the cells were suspended in alginate and seeded onto polylactic-co-glycolic acid (PLGA) scaffolds for 3D cultivation for 7 days (step 2) and the cells/alginate/PLGA complexes were further transplanted into nude mice for 8 weeks (step 3). At same time, some of complexes were cultured in vitro up to 8 weeks. At the end of step 1, cells exhibited fibroblast-like morphology and expressed chondrocyte-specific markers, Sox 9 and collagen II. During the following 8 weeks of 3D cultivation in vitro, cells displayed spherical morphology, decreased immunoreactivity to Sox-9 and increased one to collagen II, demonstrated further differentiation to mature chondrocyte. In implanted grafts, not only cells appeared typical chondrocytes shape and markers but also cartilage like tissues were formed. These results indicate that 2D-3D sequential culture in vitro is an efficient protocol to induce hESCs differentiates into chondrocytes, while the three step culture system may be an appropriate procedure to derive cartilage like tissues from hESCs. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 94A: 539-546, 2010

语种英语
WOS记录号WOS:000279482600024
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55486
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Peking Univ, Hosp 3, Reprod Med Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Stem Cell Res Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Bai, Hai Yan,Chen, Gui An,Mao, Gen Hong,et al. Three step derivation of cartilage like tissue from human embryonic stem cells by 2D-3D sequential culture in vitro and further implantation in vivo on alginate/PLGA scaffolds[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2010,94A(2):539-546.
APA Bai, Hai Yan,Chen, Gui An,Mao, Gen Hong,Song, Tian Ran,&Wang, Yan Xia.(2010).Three step derivation of cartilage like tissue from human embryonic stem cells by 2D-3D sequential culture in vitro and further implantation in vivo on alginate/PLGA scaffolds.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,94A(2),539-546.
MLA Bai, Hai Yan,et al."Three step derivation of cartilage like tissue from human embryonic stem cells by 2D-3D sequential culture in vitro and further implantation in vivo on alginate/PLGA scaffolds".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 94A.2(2010):539-546.
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