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Protective effect of paeoniflorin against oxidative stress in human retinal pigment epithelium in vitro
Xie Wankun; Yu Wenzhen; Zhao Min; Zhou Weiyan; Chen Huan; Du Wei; Huang Lvzhen; Xu, Yongsheng; Li Xiaoxin
刊名MOLECULAR VISION
2011-12-29
17期:373-78页:3512-3522
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Ophthalmology
研究领域[WOS]Biochemistry & Molecular Biology ; Ophthalmology
关键词[WOS]PROTEIN-INDUCING COMPOUND ; KINASE-DEPENDENT PATHWAYS ; HUMAN RPE CELLS ; MACULAR DEGENERATION ; INDUCED APOPTOSIS ; GENE-EXPRESSION ; ARPE-19 CELLS ; VISUAL IMPAIRMENT ; ACTIVATING MAPK ; H2O2
英文摘要

Purpose: This study was conducted to determine whether paeoniflorin (PF) could prevent H(2)O(2)-induced oxidative stress in ARPE-19 cells and to elucidate the molecular pathways involved in this protection.

Methods: Cultured ARPE-19 cells were subjected to oxidative stress with H(2)O(2) in the presence and absence of PF. The preventive effective of PF on reactive oxygen species (ROS) production and retinal pigment epithelium (RPE) cell death induced by H(2)O(2) was determined by 2′, 7′-dichlorodihydrofluorescein diacetate (H(2)DCFDA) fluorescence and 3-(4, 5dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide (MTT) assay. The ability of PF to protect RPE cells against ROS-mediated apoptosis was assessed by caspase-3 activity and 4′, 6-diamidino-2-phenylindole (DAPI) staining. Furthermore, the protective effect of PF via the mitogen-activated protein kinase (MAPK) pathway was determined by western blot analysis.

Results: PF protected ARPE-19 cells from H(2)O(2)-induced cell death with low toxicity. H(2)O(2)-induced oxidative stress increased ROS production and caspase-3 activity, which was significantly inhibited by PF in a dose-dependent manner. Pretreatment with PF attenuated H(2)O(2)-induced p38MAPK and extracellular signal regulated kinase (ERK) phosphorylation in human RPE cells, which contributed to cell viability in ARPE-19 cells.

Conclusions: This is the first report to show that PF can protect ARPE-19 cells from the cellular apoptosis induced by oxidative stress. The results of this study open new avenues for the use of PF in treatment of ocular diseases, such as age-related macular degeneration (AMD), where oxidative stress plays a major role in disease pathogenesis.

语种英语
WOS记录号WOS:000298739600005
项目编号RDB2010-25 ; 2011CB510200
资助机构Peking University People&prime ; s Hospital Research and Development Funds ; National Basic Research Program of China (973 Program)
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55497
专题北京大学第二临床医学院
北京大学第二临床医学院_眼科
作者单位Peking Univ, Peoples Hosp, Peoples Eye Inst,Minist Educ, Dept Ophthalmol,Key Lab Vis Loss & Restorat, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Xie Wankun,Yu Wenzhen,Zhao Min,et al. Protective effect of paeoniflorin against oxidative stress in human retinal pigment epithelium in vitro[J]. MOLECULAR VISION,2011,17(373-78):3512-3522.
APA Xie Wankun.,Yu Wenzhen.,Zhao Min.,Zhou Weiyan.,Chen Huan.,...&Li Xiaoxin.(2011).Protective effect of paeoniflorin against oxidative stress in human retinal pigment epithelium in vitro.MOLECULAR VISION,17(373-78),3512-3522.
MLA Xie Wankun,et al."Protective effect of paeoniflorin against oxidative stress in human retinal pigment epithelium in vitro".MOLECULAR VISION 17.373-78(2011):3512-3522.
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