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学科主题: 临床医学
题名:
Vaccination with embryonic stem cells generates effective antitumor immunity against ovarian cancer
作者: Zhang, Zujuan; Chen, Xinhua; Chang, Xiaohong; Ye, Xue; Li, Yi; Cui, Heng
关键词: ovarian cancer ; embryonic stem cell ; antitumor immunity ; immunotherapy ; cancer vaccine
刊名: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
发表日期: 2013
DOI: 10.3892/ijmm.2012.1195
卷: 31, 期:1, 页:147-153
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: BREAST-CANCER ; MOUSE MODEL ; CHEMOTHERAPY ; EXPRESSION ; VACCINES ; GROWTH
英文摘要:

To date, only a few studies have suggested that human embryonic stem cells (hESCs) might effectively immunize against colon and lung cancer. The purpose of this study was to investigate the therapeutic potential of hESCs as a vaccine to induce widespread antitumor effects in different animal models and various types of cancer. C57BL/6 mice with 1138 ovarian cancer cell and Fischer 344 rats with NuTu-19 ovarian cancer cell models were used. Fifty-four mice were divided into six groups with nine mice in each group. Each mouse was immunized with pre-inactivated hESCs (H9) or mouse embryonic stem cells (mESCs; IVP-ESI) or ID8 or phosphate-buffered saline (PBS). Twenty-four rats were divided into four groups with six rats in each group, each rat immunized with pre-inactivated hESCs (H9) or NuTu-19 or PBS. After the vaccination, each mouse was challenged with live ID8 cells subcutaneously, and each rat was challenged with live NuTu-19 cells intraperitoneally. We discovered that vaccination of mice with the hESC line H9 and the m ESC line IVP-ESI generated consistent cellular and humoral immune responses against 1138 ovarian cancer. H9 and IVP-ESI vaccinated mice obtained antitumor immune protection, and H9 vaccinated rats had the longest survival time and least distant metastases. No evidence of side-effects was observed. We also compared the immunogenicity against ovarian cancer between the hESC line, H9, and the mESC line, IVP-ESI, that derived from the inner cell mass in different species. We found that there were no significant differences between them. Furthermore, immunohistochemical staining revealed that several oncogenes and tumor suppressor genes, such as HER-2, C-myc, p53, and nm23, were expressed in H9, many of which were also shared by ovarian cancer. hESC vaccines can induce antitumor effects in two animal models and in ovarian cancer, indicating that the activity of the vaccine is universal, and, more importantly, it is safe.

语种: 英语
所属项目编号: 81072141 ; RDB2010-09
项目资助者: National Natural Science Foundation of China ; Research and Development foundation in Peking University People&prime ; s Hospital
WOS记录号: WOS:000312567000019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55506
Appears in Collections:北京大学第二临床医学院_妇科肿瘤中心_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Zhang, Zujuan,Chen, Xinhua,Chang, Xiaohong,et al. Vaccination with embryonic stem cells generates effective antitumor immunity against ovarian cancer[J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,2013,31(1):147-153.
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